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ọkọlọtọ-03

Akụkọ

Usoro transcriptome dabere Nanopore zuru oke

Usoro Nanopore na-ekewa onwe ya na usoro nhazi usoro ndị ọzọ, n'ihi na a na-agụ nucleotides ozugbo na-enweghị njikọ DNA ma na-emepụta ogologo oge na iri puku kilobases.Nke a na-enye ike ịgụpụta ndegharị n'ogologo zuru oke yana imeri ihe ịma aka ndị dị na ọmụmụ transcript.

√ Ọdịiche dị n'usoro dị ala

√ Ngụpụta cDNA zuru oke maka ọmụmụ nhazi usoro mkpụrụ ndụ ihe nketa

√ Obere data achọrọ iji kpuchie otu ọnụọgụ ederede

√ Ịmata ọtụtụ isoforms kwa mkpụrụ ndụ ihe nketa

√ Nkọwapụta okwu na ọkwa isoform

ogologo - 1

Pipeline Bioinformatics a na-ahụkarị

Ogologo-ogologo-trans-bix-1024x254

Okwu na-aga nke ọma kacha ọhụrụ na Teknụzụ Biomarker

1. Ntụle ntule nke transcriptomes zuru oke na-ekpughe Gnetum luofuense stem development dynamics.

Akwụkwọ akụkọ: Frontiers na Genetics

Ebipụtara: Maachị 2021

isiokwu: MinION |Nhọrọ ọzọ splicing |APA |lncRNA |Ederede ederede dị iche iche

Gụọ Full-ederede

2. Ntugharị Unidirectional nke obere RNA site na ome gaa na mgbọrọgwụ na heterografts interspecific.

Akwụkwọ akụkọ: Osisi okike

Ebipụtara: Jenụwarị 2021

isiokwu: Nanopore |Illumina |nchọpụta mRNA mkpanaka mkpanaka ogologo zuru oke

3. Ntụle transcriptome zuru oke nke mgbọrọgwụ asparagus na-ekpughe usoro mkpụrụ ndụ nke nnabata nnu nke arbuscular mycorrhizal fungi kpatara.

Akwụkwọ akụkọ: Environmental and Experimental Botany

Ebipụtara: Jenụwarị 2021

isiokwu: MinION |Edemede egosipụtara dị iche iche |Ihe odide ederede |Nhọrọ ọzọ splicing

4. Mgbanwe njirimara anụ ahụ na ntụgharị zuru oke nke Rose (Rosa chinensis) Mgbọrọgwụ na akwụkwọ na-azaghachi maka nrụgide oké ọkọchị.

Akwụkwọ akụkọ: Physiology Plant and Cell

Ebipụtara: Ọktoba 2020

isiokwu: PromethION |Edemede egosipụtara dị iche iche |Amụma na-arụ ọrụ |Isoforms gbawara agbawa |TF na lncRNA

5. Nyocha na ntụle zuru oke nke PacBio na usoro RNA dabeere na nanopore nke transcriptome Arabidopsis.

Akwụkwọ akụkọ: Ụzọ osisi

Ebipụtara: Eprel 2020

isiokwu: GridION |PromethION |PacBio Sequel |Illumina NovaSeq |Nhazi genome |njirimara transcript |Nhọrọ ọzọ splicing |SSR |LncRNA |Isoform quantification

Akụkọ na isi ihe Ebumnuche iji Biomarker Teknụzụ kekọrịta ikpe kachasị ọhụrụ na-aga nke ọma, na-ewepụta mmezu sayensị ọhụụ yana usoro ama ama etinyere n'oge ọmụmụ ihe ahụ.


Oge nzipu: Jan-08-2022

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