Genotyping dị elu, karịsịa na ọnụ ọgụgụ buru ibu, bụ nzọụkwụ dị mkpa na ọmụmụ ihe ọmụmụ mkpụrụ ndụ ihe nketa, nke na-enye ndabere mkpụrụ ndụ maka nchọpụta mkpụrụ ndụ ọrụ, nyocha evolushọn, wdg. ) ewebata iji belata ọnụ ahịa usoro n'otu nlele, ka na-ejigide arụmọrụ ezi uche dị na nchọpụta mkpụrụ ndụ ihe nketa.A na-enwetakarị nke a site na iwepụta iberibe mmachi n'ime oke oke enyere, nke akpọrọ ọbá akwụkwọ nnọchite anya belatara(RRL).Specific-locus amplified fragment sequencing(SLAF-Seq) bụ atumatu emepụtara onwe ya maka nchọpụta de novo SNP na SNP genotyping nke nnukwu mmadụ.
Usoro ọrụ nka
SLAF vs usoro RRL dị adị
Uru nke SLAF
Nchọpụta mkpụrụ ndụ ihe nketa dị elu- Ejikọtara ya na teknụzụ usoro ihe dị elu, SLAF-Seq nwere ike nweta ọtụtụ narị puku mkpado achọpụtara n'ime genome dum iji mezuo arịrịọ nke ọrụ nyocha dị iche iche, ma ọ bụ na genome ntụaka anyị.
Nhazi nnwale ahaziri&ngbanwe- Maka ebumnuche nyocha dị iche iche ma ọ bụ ụdị, usoro mgbaze enzymatic dị iche iche dị gụnyere otu-enzyme, dual-enzyme na multi-enzyme digestion.A ga-ebu ụzọ nyochaa usoro mgbari nri na silico iji mesie ike imepụta enzyme kacha mma.
Elu arụmọrụ na enzymatic mgbaze– Mgbari mgbaze enzymatic emebere nke ọma na-enye SLAF nke ọma karịa na chromosome.Nchịkọta iberibe nke ọma nwere ike nweta ihe karịrị 95%.
Zere usoro ugboro ugboro- Pasent nke usoro ugboro ugboro na data SLAF-Seq na-ebelata ka ọ dị ala karịa 5%, ọkachasị n'ụdị nwere nnukwu ihe nrụpụta ugboro ugboro, dị ka ọka wit, ọka, wdg.
Nrụ ọrụ bioinformatic mebere onwe ya- BMK mepụtara usoro ọrụ bioinformatic agbakwunyere nke dabara na teknụzụ SLAF-Seq iji hụ na ntụkwasị obi na izi ezi nke mmepụta ikpeazụ.
Ngwa nke SLAF
Maapụ njikọ mkpụrụ ndụ ihe nketa
Mwube maapụ mkpụrụ ndụ ihe nketa dị elu yana njirimara nke loci na-achịkwa ụdị ifuru na Chrysanthemum (Chrysanthemum x morifolium Ramat.)
Akwụkwọ akụkọ: Nnyocha Horticulture E bipụtara: 2020.7
GWAS
Nchọpụta mkpụrụ ndụ ihe nketa nke metụtara ọdịnaya isofavone na mkpụrụ soybean site na iji njikọ genome-wide na nkewa njikọ.
Akwụkwọ akụkọ: akwụkwọ akụkọ ihe ọkụkụ bipụtara: 2020.08
Genetics evolushọn
Nyocha gbasara mkpụrụ ndụ mmadụ na mgbakọ de novo na-ekpughe mmalite osikapa weedy dị ka egwuregwu evolushọn
Akwụkwọ akụkọ: Osisi Molecular E bipụtara: 2019.5
Nchịkọta Segregant (BSA)
GmST1, nke na-etinye koodu sulfotransferase, na-enye iguzogide nje mosaic soybean G2 na G3.
Akwụkwọ akụkọ: Osisi, Cell&Gburugburu Ebipụtara: 2021.04
Ntụaka
Sun X, Liu D, Zhang X, et al.SLAF-Seq: usoro dị irè nke nchọpụta SNP buru ibu na nke mkpụrụ ndụ ihe nketa site na iji usoro ihe dị elu [J].Plos otu, 2013, 8 (3): e58700
Abụ X, Xu Y, Gao K, et al.Maapụ mkpụrụ ndụ ihe nketa dị elu na njirimara nke loci na-achịkwa ụdị ifuru na Chrysanthemum (Chrysanthemum × morifolium Ramat.).Hortic Res.2020;7:108.
Wu D, Li D, Zhao X, et al.Nchọpụta nke mkpụrụ ndụ nwa akwukwo nke jikọtara ọdịnaya isoflavone na mkpụrụ soybean site na iji njikọ genome-wide na nkewa njikọ.Osisi J. 2020;104 (4): 950-963.
Sun J, Ma D, Tang L, et al.Ọnụọgụ Genomic Analysis na Mgbakọ De Novo na-ekpughe mmalite nke Weedy Rice dị ka egwuregwu evolushọn.Osisi Mol.2019;12 (5): 632-647 .Osisi Mol.2018;11 (11): 1360-1376 .
Zhao X, Jing Y, Luo Z, et al.GmST1, nke na-etinye koodu sulfotransferase, na-enye iguzogide nje mosaic soybean G2 na G3.Osisi Cell Environ.2021;10.1111/pc.14066
Oge nzipu: Jan-04-2022