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Mwepụta ngwaahịa ọhụrụ – BMKMANU S1000, Sub-cellular Level Spatial Transcriptomics – Zụrụ anọ nweta otu n'efu!

Inyocha usoro okwu mkpụrụ ndụ ihe nketa n'ebe izizi ihe dị n'ahụ dị mkpa maka ịghọta ụdị na ọrụ nke mkpụrụ ndụ ya.Agbanyeghị, ụzọ nyocha transcriptome nke dị ugbu a nwere oke dị ka ntinye dị ala ma ọ bụ mkpebi ezughi oke.The BMKMANU S1000 Spatial Chip, nke BMKMANU mepụtara, nwere ike ịchọpụta ozi nkwupụta mkpụrụ ndụ ihe dị n'ime ọnọdụ n'ime akụkụ anụ ahụ zuru oke na mkpebi subcellular, na-enye nkọwa dị nro nke nhazi anụ ahụ.

Jikere ka ị nweta ngwaahịa ọhụrụ anyị!Iji mee mmemme ntọhapụ ya, anyị na-enye ihe pụrụ icheZụrụ anọ nweta otu n'efunkwalite maka obere oge naanị.

Ngwa ngwaahịa

BMKMANU S1000 mgbawa oghere na ngwa reagent dabara gụnyere:

1) Ngwa njikarịcha anụ ahụ, nwere reagents maka njikarịcha anụ ahụ (nyocha oge kacha mma maka imezi anụ ahụ).

2) Ngwa okwu okwu Gene, nwere reagents maka ijide mRNA n'ime mpekere anụ ahụ maka nkwadebe na usoro ọbá akwụkwọ na-esote.

3) Ngwa mmalite: Gụnyere ihe nkwụnye ọkụ na ihe ndọta transcriptome.

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Ụkpụrụ nka nka

Ihe mgbawa ahụ dabere na micropores na microbeads, enwere ike ijide mRNA na ọnọdụ site na Oligo na microbeads.Oligo nwere akụkụ anọ: Read1, Spatial Barcode, UMI, na Poly(dT)VN.Mgbe etinyere anụ ahụ na mgbawa, a na-ahapụ mRNA site na anụ ahụ site na enzyme permeabilisation.Ebe ọ bụ na ọtụtụ ngwụcha mRNA 3' nwere ọdụ Poly-A, Oligo ji Poly(dT) VN jide ha.Mgbe nkwalite RT-PCR na cDNA gasịrị, nkwadobe na nhazi ọbá akwụkwọ, a na-enweta nchọta ọnọdụ ohere site na oghere oghere.Nke a na-enye ohere maka nyocha okwu mkpụrụ ndụ ihe nketa na ọnọdụ oghere yana ụdị anụ ahụ dị iche iche.

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Uru ngwaahịa

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Ngwa

Enwere ike itinye ya n'ọrụ n'ọtụtụ ebe nyocha nke usoro ndu na ahụike, gụnyere etuto, ọrịa, immunology, na bayoloji mmepe, na-enyere aka nweta ọganihu ọhụrụ na mpaghara ndị a na nyocha okwu gbasara ohere na mkpebi subcellular.

● Tumor na Ọrịa:
Heterogeneity oghere na microenvironment nke etuto ahụ na ọrịa
Mmalite na mmepe nke etuto ahụ na ọrịa
Nzaghachi ọgwụgwọ nke etuto ahụ na ọrịa

● Ihe omumu ihe omumu
Spatiotemporal atlas nke akụkụ ahụ
Usoro nchịkwa nke mkpụrụ ndụ n'oge mmepe

●Nzaghachi Nchegbu
Nzaghachi nrụgide biotic
Nzaghachi nrụgide Abiotic

●Immunology
Mmeghachi omume mgbochi na ntinye akụkụ ahụ
Usoro mgbochi ọrịa etuto na ọrịa
Pathogenesis nke ọrịa autoimmune

● Nyocha nyocha ọgwụ
Usoro nke iguzogide ọgwụ
Nnyocha na mmepe nke ọgwụ ọhụrụ

Okwu na Demo Data

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BMKMANU S1000 ka akwadoro ịrụ ọrụ n'ọtụtụ narị ikpe n'ofe anụ ahụ dị iche iche.

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Zitere anyị ozi gị: