Ngwaahịa

Fungal Genome

Teknụzụ Biomarker na-enye nyocha genome, ezigbo genome na genome zuru oke nke fungal dabere na ebumnuche nyocha akọwapụtara.Enwere ike nweta usoro genome, mgbakọ na nkọwa ọrụ site na ijikọta usoro nke ọgbọ na-abịa + usoro ọgbọ nke atọ iji nweta mgbakọ genome dị elu.Enwere ike iji teknụzụ Hi-C rụọ ọrụ iji kwado mgbakọ genome na ọkwa chromosome.

Platform:PacBio Sequel II

Nanopore PromethION P48

Illumina NovaSeq Platform

Kpọtụrụ anyị

Nkọwa ọrụ

Nsonaazụ ngosi

Uru ọrụ

● Ọtụtụ usoro usoro dị maka ebumnuche nyocha dị iche iche

● Ahụmahụ nke ukwuu na mgbakọ genome nke fungi nwere ihe karịrị 10,000 pene-zuru ezu genome gbakọtara.

● Ọkachamara mgbe-sale oru nkwado otu na-emezu ọzọ kpọmkwem nnyocha mkpa.

Nkọwapụta ọrụ

Ọrụ	Usoro usoro	Akwadoro mma n'anya	Atụmatụ ngbanwe oge
Fungal map mara mma	Illumina 50X+ Nanopore 100X	Na-aga n'ihu N50≥2 Mb	35 ụbọchị ọrụ
Fungal map mara mma	PacBio HiFi 30X	Na-aga n'ihu N50≥2 Mb	35 ụbọchị ọrụ
Map zuru ezu nke pene fungal	Illumina 50X+ Nanopore 100X(Pacbio HiFi 30X)+Hi-C 100X	Oke anchoring chromosome>90%	45 ụbọchị ọrụ

Nyocha bioinformatics

● Njikwa ogo data raw

● Mgbakọ genome

● Nyocha akụkụ genome

● Nkọwa ọrụ mkpụrụ ndụ ihe nketa

● Nyocha mkpụrụ ndụ ihe atụ

Ihe nlele chọrọ na nnyefe

Ihe atụ chọrọ:

MakaDNA wepụ:

Ụdị Nlereanya	Ọnụ ego	Ntinye uche	Ịdị ọcha
DNA wepụ	1.2 μg	20 ng/μl	OD260/280 = 1.6-2.5

Maka nlele anụ ahụ:

Ụdị ihe atụ

Ọgwụgwọ nlele akwadoro

Nlereanya nchekwa na mbupu

Fungus Unicellular

Lelee yist n'okpuru microscope wee chịkọta ha n'oge ngosipụta ha

Nyefee omenala (nwere ihe dị ka 3-4.5e9 mkpụrụ ndụ) n'ime 1.5 ma ọ bụ 2 ml ependorf.(Nọgide na ice)

Centrifuge tube maka 1 min na 14000 g iji kpokọta nje bacteria ma wepụ ihe dị elu nke ọma.

Mechie tube ahụ ma kpoo nje bacteria na mmiri mmiri nitrogen maka ọ dịkarịa ala 1-3 h.Chekwaa tube ahụ na friji -80 ℃.

Debe ihe nlele ahụ na nitrogen mmiri mmiri maka awa 3-4 ma chekwaa na nitrogen mmiri mmiri ma ọ bụ -80 ogo na ndoputa ogologo oge.Achọrọ mbupu ihe atụ na ice-akọrọ.

Ọrịa ero

A na-atụ aro ka anụ ahụ na-eto eto na-arụsi ọrụ ike.

Jiri mmiri na-enweghị endotoxin sachaa anụ ahụ, wee 70% ethonal.

Chekwaa ihe nlele na tubes cryo.