Illumina na BGI
Atụmatụ
●Platform:Illumina NovaSeq 6000, NovaSeq, HiSeq X iri na BGI-DNB-T7
●Ụdị usoro:PE50, PE100, PE150, PE250
●Njikwa ogo nke ọba akwụkwọ tupu usoro
●Usoro nnyefe data na QC:nnyefe nke akụkọ QC na data raw na usoro fastq mgbe demultiplexing na nzacha Q30 na-agụ.
Uru ọrụ
●Ọdịiche nke ọrụ usoro:onye ahịa nwere ike họrọ usoro site n'okporo ụzọ, cell flow ma ọ bụ ọnụọgụ data.
●Mgbanwe nke nyiwe:Enwere ike ibufe ọba akwụkwọ DNB na nyiwe Illumina
●Ahụmahụ dị ukwuu na ikpo okwu usoro usoro Illumina:na ọtụtụ puku ọrụ mechiri emechi nwere ụdị dị iche iche.
●Mbufe nke usoro QC akụkọ:ya na metrik dị mma, izi ezi data na ịrụ ọrụ n'ozuzu nke usoro nhazi.
●Usoro usoro nke tozuru oke:na obere oge mgbanwe.
●Njikwa ogo siri ike: anyị na-emejuputa iwu QC siri ike iji kwe nkwa ịnye nsonaazụ dị elu mgbe niile.
Ihe atụ chọrọ*
Usoro akụkụ akụkụ
| Ọnụ ego data (X) | Ntinye uche (qPCR/nM) | Olu |
| X ≤ 50 Gb | ≥2 nM | ≥ 20 μl |
| 50 Gb ≤ X <100 Gb | ≥3 nM | ≥ 20 μl |
| X ≥ 100 Gb | ≥4 nM | ≥ 20 μl |
Otu ụzọ (Ilumina)
| Platform | Ntinye uche (qPCR/nM) | Olu |
| HiSeq X iri | ≥2 nM | ≥ 20 μl |
| NovaSeq 6000 SP | ≥1 nM | ≥ 25 μl |
| NovaSeq 6000 S4 | ≥ 1.5 nM | ≥ 25 μl |
| NovaSeq X | ≥ 1.5 nM | ≥ 25 μl |
| BGI-DNBSEQ-T7 | ≥ 1.5 nM | ≥ 25 μl |
Na mgbakwunye na itinye uche na mkpokọta ego, a chọkwara ụkpụrụ kacha elu kwesịrị ekwesị.
Biko kpọtụrụ anyị ma ọ bụrụ na nlele gị emezughị ihe mmalite ihe achọrọ.
Usoro ọrụ ọrụ
Njikwa ogo ụlọ akwụkwọ
Usoro
Njikwa ogo data
Nnyefe oru ngo
Akụkọ ụlọ akwụkwọ QC
A na-enye akụkọ maka ogo ụlọ akwụkwọ ahụ tupu usoro nhazi, nyochaa ego ọbá akwụkwọ, na nkewa.
Usoro nke akụkọ QC
Tebụl 1. Statistics na usoro data.
| NJ nlele | BMKID | Raw na-agụ | Data raw (bp) | Ọgụgụ dị ọcha (%) | Q20(%) | Q30(%) | GC(%) |
| C_01 | BMK_01 | 22,870,120 | 6,861,036,000 | 96.48 | 99.14 | 94.85 | 36.67 |
| C_02 | BMK_02 | 14,717,867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Ọgụgụ 1. Ogo nkesa tinyere na-agụ na nke ọ bụla sample
Ọgụgụ 2. Isi nkesa ọdịnaya
Ọgụgụ 3. Nkesa nke ọdịnaya agụ na usoro data
