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ọkọlọtọ-03

Ngwaahịa

Usoro mgbochi mgbochi Chromatin (ChiP-seq)

Usoro mgbochi mgbochi Chromatin (ChiP-seq) onyonyo egosipụtara
  • Usoro mgbochi mgbochi Chromatin (ChiP-seq)
  • Usoro mgbochi mgbochi Chromatin (ChiP-seq)

ChIP-Seq na-enye nkọwapụta genome-obosara nke ebumnuche DNA maka mgbanwe histone, ihe odide ederede, yana protein ndị ọzọ metụtara DNA.Ọ na-ejikọta nhọrọ nke chromatin immuno-precipitation (ChiIP) iji nwetaghachi ogige protein-DNA kpọmkwem, yana ike nke usoro ọgbọ na-esote (NGS) maka usoro ntinye dị elu nke DNA enwetara.Na mgbakwunye, n'ihi na a na-anata ogige protein-DNA site na mkpụrụ ndụ dị ndụ, enwere ike iji saịtị njikọ n'ụdị cell na anụ ahụ dị iche iche, ma ọ bụ n'okpuru ọnọdụ dị iche iche.Ngwa sitere na ụkpụrụ ntụgharị gaa na ụzọ mmepe gaa na usoro ọrịa na karịa.

Platform: Illumina NovaSeq Platform


Nkọwa ọrụ

Bioinformatics

Nsonaazụ ngosi

Uru ọrụ

● Platform: Illumina NovaSeq Platform, PE150

● Ụdị ọba akwụkwọ: 100-500bp tinye DNA ọba akwụkwọ (dabere na nkesa kacha elu)

● Usoro nhazi usoro: Ejikọtara- Ọgwụgwụ 150 bp

● Kwado mmepụta data: 6 Gb raw data/ sample.

Ihe atụ chọrọ

Ọnụọgụ DNA: ≥ 70 ng, isi kacha elu nke 100 ruo 500 bp

Ihe atụ: ≥ 10 μl

OD260/280 = 1.8 ruo 2.0 na-enweghị mmebi ma ọ bụ mmetọ RNA.

Usoro ọrụ nnwale

图片1

Usoro ọrụ ọrụ

nnyefe sample

Nbufe ihe atụ

Nkwadebe ụlọ akwụkwọ

Ịrụ ụlọ akwụkwọ

Usoro

Usoro

Nyocha data

Nyocha data

Mgbe ire Ọrụ

Mgbe-ire ọrụ

  • Nke gara aga:
  • Osote:

    • 表观-01

    1. Nkesa nkwalite kacha elu

    官网图1

     

    2. MA ibé nke di iche iche elu

    官网图2

     

    3.KEGG nkọwa

    官网图3

     

    4. Gaa nhazi

    官网图4

    nweta ntinye okwu

    Dee ozi gị ebe a ziga anyị ya

    Ngwaahịaedemede

    Zitere anyị ozi gị:

    Pịa tinye ka ịchọọ ma ọ bụ ESC mechie