Nnukwu nyocha Segregant
Uru ọrụ
Takagi et al., Akwụkwọ akụkọ ihe ọkụkụ, 2013
● Ọnọdụ ziri ezi: Ịgwakọta nnukwu mmadụ na 30+30 ruo 200+200 mmadụ iji belata mkpọtụ ndabere;amụma mpaghara ndoro-ndoro ochichi mutatant na-abụghị synonymous.
● Ntụle zuru oke: Nkọwapụta mkpụrụ ndụ ihe omimi dị omimi, gụnyere NR, SwissProt, GO, KEGG, COG, KOG, wdg.
● Oge ntụgharị ọsọ ọsọ: Ndepụta mkpụrụ ndụ ihe nketa ngwa ngwa n'ime ụbọchị ọrụ 45.
● Ahụmahụ dị ukwuu: BMK enyela aka n'ọtụtụ puku àgwà n'ime obodo, na-ekpuchi ụdị dị iche iche dị ka ihe ọkụkụ, ngwaahịa mmiri, ọhịa, okooko osisi, mkpụrụ osisi, wdg.
Nkọwapụta ọrụ
Ndị mmadụ:
Na-ekewapụta ọmụmụ nke ndị nne na nna nwere ụdị phenotype na-emegide.
eg F2 ụmụ, Backcrossing (BC), Recombinant inbred line(RIL)
Ngwakọta ọdọ mmiri
Maka àgwà qualitative: 30 ruo 50 ndị mmadụ (opekempe 20) / nnukwu
Maka ọnụọgụ ọnụọgụ: n'elu 5% ruo 10% ndị nwere ụdị phenotypes dị oke egwu na ọnụ ọgụgụ mmadụ dum (opekempe 30+30).
Omimi usoro akwadoro
Opekempe 20X/ nne na nna na 1X/mkpụrụ n'otu n'otu (dịka maka ịgwakọta ọdọ mmiri nke 30+30 onye, usoro omimi ga-abụ 30X kwa nnukwu)
Nyocha bioinformatics
● Usoro mkpụrụ ndụ ihe nketa niile
● Nhazi data
● SNP/Indel na-akpọ
● Nyocha mpaghara ndị na-eme ntuli aka
● Nkọwa gbasara mkpụrụ ndụ ihe nketa
Ihe nlele chọrọ na nnyefe
Ihe atụ chọrọ:
Nucleotides:
| gDNA sample | Ihe atụ anụ ahụ |
| Ntinye uche: ≥30 ng/μl | Osisi: 1-2 g |
| Ọnụ ego: ≥2 μg (Mpịakọta ≥15 μl) | Ụmụ anụmanụ: 0.5-1 g |
| Ịdị ọcha: OD260/280 = 1.6-2.5 | Ọbara zuru oke: 1.5 ml |
Usoro ọrụ ọrụ
Nnwale imewe
Nbufe ihe atụ
mmịpụta RNA
Ịrụ ụlọ akwụkwọ
Usoro
Nyocha data
Mgbe-ire ọrụ
1.Association analysis base on Euclidean Distance (ED) iji chọpụta mpaghara nwa akwukwo.Na ọnụ ọgụgụ na-esonụ
X-axis: nọmba chromosome;Ntụpọ ọ bụla na-anọchi anya uru ED nke SNP.Ahịrị ojii kwekọrọ na uru ED dabara adaba.Uru ED dị elu na-egosi njikọ dị ịrịba ama n'etiti saịtị na phenotype.Ahịrị dash na-acha uhie uhie na-anọchi anya ọnụ ụzọ mkpakọrịta dị mkpa.
2.Association analysis dabeere na ọ dịghị SNP-index
X-axis: nọmba chromosome;Ntụpọ ọ bụla na-anọchite uru SNP-index.Ahịrị ojii na-anọchi anya uru SNP-index dabara adaba.Ka ọnụ ahịa ya na-abawanye, ka mkpakọrịta ahụ dị ịrịba ama.
Okwu BMK
Isi njirimara ọnụọgụ ọnụọgụgụ locus Fnl7.1 na-akọwapụta protein embryogenesis nke mbubreyo jikọtara ya na ogologo olu olu na kukumba.
Ebipụtara: Akwụkwọ akụkọ ihe ọkụkụ nke biotechnology, 2020
Atụmatụ usoro:
Nne na nna (Jin5-508, YN): Gburugburu genome resequencing maka 34 × na 20 ×.
Ọdọ mmiri DNA (50 ogologo olu na 50 dị mkpụmkpụ): Na-achọgharị maka 61 × na 52 ×
Nsonaazụ igodo
N'ime ọmụmụ ihe a, ewepụtara ọnụ ọgụgụ mmadụ (F2 na F2: 3) site na ịgafe ahịrị kukumba ogologo olu Jin5-508 na YN dị mkpụmkpụ.Ndị mmadụ 50 dị oke ogologo na mmadụ iri ise dị mkpụmkpụ rụrụ ọdọ mmiri DNA abụọ.Achọpụtara nnukwu mmetụta QTL na Chr07 site na nyocha BSA na nkewa QTL ọdịnala.A na-ebelata mpaghara ndị a na-eme ntuli aka site na mma-map, mkpụrụ ndụ okwu ọnụ na nnwale transgenic, nke kpughere mkpụrụ ndụ ihe nketa na ịchịkwa ogologo olu, CsFnl7.1.Na mgbakwunye, polymorphism na mpaghara CsFnl7.1 na-akwalite ka achọpụtara na ejikọtara ya na nkwupụta kwekọrọ.Nyocha phylogenetic ndị ọzọ tụrụ aro na mpaghara Fnl7.1 yikarịrị ka ọ ga-esi na India pụta.
 QTL-mapping na nyocha BSA iji chọpụta mpaghara nwa akwukwo jikọtara ya na ogologo olu kukumba |  Achọpụtara profaịlụ LOD nke ogologo olu kukumba QTL na Chr07 |
Xu, X., et al."Ụdị njirimara ọnụọgụ ọnụọgụ nke bụ isi Fnl7.1 na-akọwapụta protein embryogenesis nke mbubreyo jikọtara ya na ogologo olu olu na kukumba."Akwụkwọ akụkọ ihe ọkụkụ nke biotechnology 18.7 (2020).
