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BMKCloud

  • PacBio-Full-length Transcriptome (Non-Reference)

    Transcriptome-ogologo zuru oke nke PacBio (anaghị ntụnye aka)

    Amplicon (16S / 18S / ITS) n'elu ikpo okwu na-mepụtara na afọ nke ahụmahụ na microbial diversity oru ngo analysis, nke nwere ọkọlọtọ isi analysis na ahaziri analysis: isi analysis na-ekpuchi mainstream analysis ọdịnaya nke ugbu a microbial nnyocha, na analysis ọdịnaya bụ ọgaranya na keukwu. na nsonaazụ nyocha gosipụtara n'ụdị akụkọ akụkọ;Ọdịnaya nke nyocha ahaziri iche dị iche iche.Enwere ike ịhọpụta ihe nlele ma nwee ike ịtọ ntọala n'ụzọ dị nro dị ka akụkọ nyocha na ebumnuche nyocha siri dị, iji ghọta ihe achọrọ ahaziri iche.Sistemụ arụmọrụ Windows, dị mfe na ngwa ngwa.

  • PacBio-Full-length Transcriptome (Non-Reference)

    Transcriptome-ogologo zuru oke nke PacBio (anaghị ntụnye aka)

    Inweta data sequencing nke Pacific Biosciences (PacBio) dị ka ntinye, ngwa a nwere ike ịchọpụta usoro transcript zuru oke (na-enweghị mgbakọ).Site na ịdepụta usoro ogologo zuru oke megide genome ntụaka, enwere ike ịhazi transcript site na mkpụrụ ndụ amaara ama, transcripts, mpaghara koodu, wdg. N'okwu a, enwere ike nweta njirimara ziri ezi nke ihe owuwu mRNA, dị ka mkpọsa ọzọ, wdg.Nyocha ọnụ na NGS transcriptome sequencing data na-enyere nkọwa zuru ezu na nkọwa zuru oke na okwu n'ọkwa transcript, nke na-erite uru dị ukwuu n'okwu dị iche iche na nyocha ọrụ.

  • Toolkits

    Ngwa ngwa

    BMKCloud bụ a na-eduga bioinformatic ikpo okwu na-enye onye-nkwụsị ngwọta maka genomic mmemme, nke a na ọtụtụ tụkwasịrị obi site na-eme nnyocha na dị iche iche arena gụnyere ọgwụ, ugbo, gburugburu ebe obibi, wdg BMKCloud agba mbọ na-enye integrated, pụrụ ịdabere na oru oma ọrụ gụnyere bioinformatic analysis nyiwe na ngwaọrụ. , Mgbakọ akụrụngwa, ọha data data, bioinformtic online ọmụmụ, wdg BMKCloud nwere dị iche iche ugboro ugboro bioinformatic ngwá ọrụ nke gụnyere gene annotation, evolushọn mkpụrụ ndụ ihe nketa ngwá ọrụ, ncRNA, data àgwà akara, mgbakọ, alignment, data mmịpụta, mutations, ọnụ ọgụgụ, Figure generator, usoro. nyocha, wdg.

  • small RNA

    obere RNA

    Obere RNA bụ ụdị RNA dị mkpụmkpụ na-abụghị koodu nke nwere ogologo 18-30 nt, gụnyere miRNA, siRNA na piRNA.A kọwapụtara obere RNA ndị a nke ukwuu na ha na-etinye aka na usoro ndu dị iche iche dị ka mmebi mRNA, mgbochi ntụgharị, nhazi heterochromatin, wdg. A na-etinye nyocha nke usoro obere RNA n'ọtụtụ ebe n'ọmụmụ ihe gbasara mmepe anụmanụ / osisi, ọrịa, nje, wdg. Obere RNA. usoro nyocha usoro ihe mejupụtara nyocha ọkọlọtọ na ngwuputa data dị elu.Na ndabere nke data RNA-seq, nyocha ọkọlọtọ nwere ike nweta njirimara na amụma miRNA, amụma mkpụrụ ndụ ihe nketa miRNA, nkọwa na nyocha okwu.Nyocha dị elu na-enyere aka nchọ na mmịpụta miRNA ahaziri ahaziri, ọgbọ Venn eserese, miRNA na ụlọ netwọkụ ezubere iche.

  • NGS-WGS (Illumina/BGI)

    NGS-WGS (Ilumina/BGI)

    NGS-WGS bụ usoro nyocha usoro nhazigharị genome dum, nke emepụtara na ndabere nke ahụmịhe bara ụba na Teknụzụ Biomarker.Ikpo okwu a dị mfe iji mee ihe na-enye ohere ntinye ngwa ngwa nke usoro nyocha agbakwunyere site na ịtọ ntọala ole na ole, nke dabara maka data usoro DNA sitere na ikpo okwu Illumina na usoro usoro BGI.A na-etinye ikpo okwu a na ihe nkesa kọmputa na-arụ ọrụ dị elu, nke na-enye ike nyocha data nke ọma na obere oge.Ngwuputa data ahaziri onwe ya dị na ndabere nke nyocha ọkọlọtọ, gụnyere ajụjụ mkpụrụ ndụ agbanwe agbanwe, imewe PCR primer, wdg.

  • mRNA(Reference)

    mRNA(ntụaka)

    Transcriptome bụ njikọ dị n'etiti ozi mkpụrụ ndụ ihe nketa na proteome nke ọrụ ndu.Usoro nhazi ọkwa ederede bụ nke kachasị mkpa na nke a kacha mụọ n'ọtụtụ ebe usoro ụkpụrụ nke ntule.Usoro transcriptome nwere ike ịhazi transcriptome n'oge ọ bụla n'oge ma ọ bụ n'ọnọdụ ọ bụla, yana mkpebi ziri ezi na otu nucleotide. Ọ nwere ike gosipụta ọkwa nke ndegharị mkpụrụ ndụ ihe nketa, n'otu oge chọpụta ma chọpụta transcript dị ụkọ na nke nkịtị, ma nye ozi nhazi nke sample kpọmkwem transcripts.

    Ka ọ dị ugbu a, a na-eji teknụzụ transcriptome sequencing n'ọtụtụ ebe na agronomy, nkà mmụta ọgwụ na mpaghara nyocha ndị ọzọ, gụnyere ụkpụrụ mmepe anụmanụ na osisi, mgbanwe gburugburu ebe obibi, mmekọrịta mgbochi ọrịa, mkpụrụ ndụ ihe nketa, ụdị evolushọn mkpụrụ ndụ ihe nketa na tumor na nchọpụta ọrịa mkpụrụ ndụ ihe nketa.

  • Metagenomics (NGS)

    Metagenomics (NGS)

    Emebere ikpo okwu nyocha a maka nyocha data metagenomic shotgun dabere na ahụmịhe afọ.Ọ mejupụtara arụ ọrụ agbakwunyere nke nwere nyocha metagenomics dị iche iche a na-achọkarị gụnyere nhazi data, ọmụmụ ọkwa ụdị, ọmụmụ ihe n'ọkwa nke mkpụrụ ndụ ihe nketa, metagenome binning, wdg. Na mgbakwunye, ngwaọrụ Ngwuputa data ahaziri iche dị n'elu usoro nyocha ọkọlọtọ, gụnyere mkpụrụ ndụ ihe nketa na ajụjụ ụdị. , ntọala paramita, imepụta ọnụ ọgụgụ ahaziri iche, wdg.

  • LncRNA

    LncRNA

    Ogologo RNA ndị na-abụghị koodu (lncRNA) bụ ụdị ederede nwere ogologo ogologo karịa 200 nt, nke na-enweghị ike itinye koodu protein.Mgbakọ akaebe na-egosi na ọtụtụ n'ime lncRNA nwere ike ịrụ ọrụ.Teknụzụ usoro nrụpụta dị elu na ngwaọrụ nyocha bioinformatic na-enye anyị ike ikpughe usoro lncRNA na idobe ozi nke ọma ma duga anyị ịchọpụta lncRNA nwere ọrụ nhazi dị mkpa.BMKCloud dị mpako ịnye ndị ahịa anyị usoro nyocha usoro lncRNA iji nweta nyocha ngwa ngwa, ntụkwasị obi na mgbanwe lncRNA.

  • GWAS

    GWAS

    Ọmụmụ ihe gbasara njikọ genome-wide (GWAS) na-achọ ịchọpụta ụdị mkpụrụ ndụ ihe nketa (genotype) nke jikọtara ya na njirimara ụfọdụ (phenotype).Ọmụmụ GWA na-enyocha akara mkpụrụ ndụ ihe nketa na-agafe genome nke ọnụ ọgụgụ mmadụ buru ibu ma buru amụma banyere mkpakọrịta genotype-phenotype site na nyocha ọnụ ọgụgụ na ọkwa ọnụ ọgụgụ mmadụ.Usoro nke genome zuru oke nwere ike ịchọpụta ụdị mkpụrụ ndụ ihe nketa niile.N'ijikọ na data phenotypic, GWAS nwere ike hazie iji chọpụta phenotype metụtara SNPs, QTL na mkpụrụ ndụ ihe nketa, nke na-akwadosi ike ịzụlite anụmanụ/osisi ọgbara ọhụrụ.SLAF bụ atụmatụ usoro usoro mkpụrụ ndụ ihe dị mfe nke mebere onwe ya, nke na-achọpụta akara nrịbama kesara genome, SNP.Ndị SNP ndị a, dị ka ihe nrịbama mkpụrụ ndụ ihe nketa molekụla, enwere ike ịhazi maka ọmụmụ mkpakọrịta nwere àgwà ezubere iche.Ọ bụ atụmatụ dị ọnụ ahịa iji chọpụta àgwà dị mgbagwoju anya metụtara ụdị mkpụrụ ndụ ihe nketa.

  • Nanopore Full-length transcriptomics

    Nanopore transcriptomics zuru oke

    Mgbagwoju anya na mgbanwe dị iche iche dị n'ime ihe dị ndụ bụ usoro mkpụrụ ndụ ihe dị mkpa maka ịhazi okwu mkpụrụ ndụ ihe nketa na ụdị protein dị iche iche.Nchọpụta nke ọma nke nhazi ederede bụ ihe ndabere maka nyocha miri emi nke usoro nhazi okwu mkpụrụ ndụ ihe nketa.Ikpo okwu usoro Nanopore ewetala ọmụmụ ihe ederede na ọkwa isoform nke ọma.Ezubere ikpo okwu nyocha a iji nyochaa data RNA-Seq ewepụtara na ikpo okwu Nanopore na ntọala genome, nke na-enweta nyocha qualitative na quantitative na ma ọkwa mkpụrụ ndụ ihe nketa yana ọkwa transcript.

     

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