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Illumina Sequencing Provider
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Whole transcriptome sequencing – Illumina – Biomarker Detail:
Service Advantages
Sample Requirements and Delivery
Sample Requirements:
Nucleotides:
Purity |
Integrity |
Contamination |
Amount |
OD260/280≥1.7-2.5; OD260/230≥0.5-2.5; |
For plants: RIN≥6.5; For animals: RIN≥7; 28S/18S≥1.0; limited or no baseline elevation |
Limited or no protein or DNA contamination shown on gel. |
Conc. ≥100 ng/μl; Volumn ≥ 10 μl; Total ≥ 2 μg |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended.
Blood samples:
PA×geneBloodRNATube;
6mLTRIzol and 2mL blood(TRIzol:Blood=3:1)
Recommended Sample Delivery
Container:
2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment:
1.Dry-ice: Samples need to be packed in bags and buried in dry-ice.
2.RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow
![logo_01](/uploads/logo_012.jpg)
Experiment design
![logo_02](/uploads/logo_022.jpg)
Sample delivery
![logo_03](/uploads/logo_032.jpg)
RNA extraction
![logo_04](/uploads/logo_042.jpg)
Library construction
![logo_05](/uploads/logo_052.jpg)
Sequencing
![logo_06](/uploads/logo_062.jpg)
Data analysis
![logo_07](/uploads/logo_072.jpg)
After-sale services
Product detail pictures:
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Hot sale Factory Mrna Seq Provider -
Whole transcriptome sequencing – Illumina – Biomarker , The product will supply to all over the world, such as: Belarus
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