Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)
Cov ntsiab lus kev pabcuam
Tswv yim
Kev khiav haujlwm
Kev Pabcuam Zoo
High marker discovery efficiency- High-throughput sequencing thev naus laus zis pab SLAF-Seq hauv kev tshawb pom ntau pua txhiab tus cim npe hauv tag nrho cov genome.
Tsawg dependence rau genome- Nws tuaj yeem siv rau hom kab nrog lossis tsis muaj kev siv genome.
Kev tsim qauv yooj yim- Ib leeg-enzyme, dual-enzyme, ntau-enzyme digestion thiab ntau hom enzymes, txhua tus tuaj yeem xaiv los ua kom tiav cov hom phiaj tshawb fawb sib txawv lossis hom.Kev ntsuam xyuas ua ntej hauv silico yog siv los ua kom paub tseeb tias kev tsim cov enzyme zoo.
Txhim kho enzymatic digestion- Kev sim ua ntej tau ua tiav los txhim kho cov xwm txheej, uas ua rau kev sim ua haujlwm ruaj khov thiab txhim khu kev qha.Fragment collection efficiency tuaj yeem ua tiav ntau dua 95%.
SLAF cov cim npe sib npaug- SLAF cov cim npe sib npaug sib npaug hauv txhua qhov chromosomes rau qhov loj tshaj plaws, ua tiav qhov nruab nrab ntawm 1 SLAF ib 4 kb.
Muaj txiaj ntsig zam kev rov ua dua- Cov ntaub ntawv rov ua dua hauv SLAF-Seq cov ntaub ntawv raug txo qis dua 5%, tshwj xeeb tshaj yog nyob rau hauv cov tsiaj uas muaj qib siab rov ua dua, xws li nplej, pob kws, thiab lwm yam.
Kev paub dav dav- Ntau tshaj 2000 kaw SLAF-Seq cov haujlwm ntawm ntau pua hom tsiaj npog cov nroj tsuag, tsiaj txhu, noog, kab, kab mob aqua, thiab lwm yam.
Tus kheej-tsim bioinformatic workflow- Ib qho kev sib koom ua haujlwm bioinformatic rau SLAF-Seq yog tsim los ntawm BMKGENE kom ntseeg tau tias muaj kev ntseeg siab thiab raug ntawm cov khoom siv kawg.
Service Specifications
| Platform | Conc.(ng/gl) | Tag nrho (ug) | OB 260/280 |
| Illumina NovaSeq | > 35 | >1.6(Nqe> 15μl) | 1.6-2.5 Nws |
Pom zoo Sequencing Strategy
Sequencing qhov tob: 10X / Tag
| Genome Loj | Pom zoo SLAF Tags |
| <500 Mb | 100K los yog WGS |
| 500 Mb- 1 Gb | 100 K |
| 1 Gb - 2 Gb | 200 K |
| Giant los yog complex genomes | 300-400K |
| Daim ntawv thov
| Pom zoo Pej xeem Scale
| Sequencing lub tswv yim thiab qhov tob
| |
| Qhov tob
| Tag Number
| ||
| GWAS
| Qauv nab npawb ≥ 200
| 10 X
|
Raws li genome loj
|
| Genetic Evolution
| Cov neeg ntawm txhua tus pab pawg ≥ 10; tag nrho cov qauv ≥ 30
| 10 X
| |
Pom zoo coj mus kuaj
Ntim: 2 ml centrifuge tube
Rau feem ntau ntawm cov qauv, peb pom zoo kom tsis txhob khaws cia hauv ethanol.
Sample labeling: Cov qauv yuav tsum tau sau kom meej meej thiab zoo ib yam rau xa cov qauv ntaub ntawv.
Kev Thauj Khoom: Dry-ice: Cov qauv yuav tsum tau ntim rau hauv hnab ua ntej thiab muab faus rau hauv cov dej khov qhuav.
Kev Pabcuam Ua Haujlwm
Qauv QC
Pilot kev sim
SLAF-kev sim
Kev npaj tsev qiv ntawv
Sequencing
Kev txheeb xyuas cov ntaub ntawv
Kev pab cuam tom qab muag
1. Kev txheeb xyuas ntawm daim ntawv qhia kev tshwm sim
2. SLAF marker kev txhim kho
3. Variation annotation
| Xyoo | Phau ntawv | IF | Title | Daim ntawv thov |
| 2022 | Ntuj kev sib txuas lus | 17.694 ib | Genomic hauv paus ntawm giga-chromosomes thiab giga-genome ntawm tsob ntoo peony Paeonia ostii | SLAF-GWAS |
| 2015 | Tshiab Phytologist | 7.433 ib | Domestication footprints thauj cov cheeb tsam genomic ntawm agronomic tseem ceeb hauv taum mog | SLAF-GWAS |
| 2022 | Phau ntawv Journal of Advanced Research | 12.822 Nws | Genome-wide dag introgressions ntawm Gossypium barbadense rau G. hirsutum qhia superior loci rau ib txhij txhim kho ntawm paj rwb fiber zoo thiab tawm los yam ntxwv | SLAF-Evolutionary noob caj noob ces |
| 2019 | Molecular Cog | 10.81 Nws | Population Genomic Analysis thiab De Novo Assembly nthuav tawm lub hauv paus chiv keeb ntawm Weedy Rice raws li Evolutionary Game | SLAF-Evolutionary noob caj noob ces |
| 2019 | Nature Genetics | 31.616 ib | Genome sequence thiab genetic diversity ntawm hom carp, Cyprinus carpio | SLAF-Linkage map |
| Xyoo 2014 | Nature Genetics | 25.455 Nws | Lub genome ntawm cov txiv laum huab xeeb muab kev nkag siab rau hauv legume karyotypes, polyploid evolution thiab qoob loo domestication. | SLAF-Linkage map |
| 2022 | Plant Biotechnology Journal | 9.803 ib | Kev txheeb xyuas ntawm ST1 qhia txog kev xaiv uas cuam tshuam nrog kev sib tsoo ntawm cov noob morphology thiab roj cov ntsiab lus thaum soybean domestication | SLAF-Marker kev txhim kho |
| 2022 | International Journal of Molecular Sciences | 6.208 ib | Kev txheeb xyuas thiab DNA Marker Kev Txhim Kho rau Nplej-Leymus mollis 2Ns (2D) Disomic Chromosome Hloov | SLAF-Marker kev txhim kho |
