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DNA/RNA sequencing - PacBio Sequencer
PacBio sequencing platform yog ib tug ntev-nyeem sequencing platform, uas yog tseem lub npe hu ua ib tug thib peb-Tim sim no Sequencing (TGS) technologies.Cov thev naus laus zis thev naus laus zis, ib leeg-molecule real-time (SMRT), txhawb kev tsim cov ntawv nyeem nrog kaum ntawm kilo-base ntev.Nyob rau hauv lub hauv paus ntawm "Sequencing-by-Synthesis", ib qho kev daws teeb meem nucleotide yog ua tiav los ntawm Zero-mode waveguide (ZMW), qhov twg tsuas yog ntim ntim hauv qab (qhov chaw ntawm molecule synthesis), yog illuminated.Tsis tas li ntawd, SMRT sequencing feem ntau zam qhov kev tsis sib haum xeeb tshwj xeeb hauv NGS system, uas feem ntau ntawm PCR amplification cov kauj ruam tsis tas yuav tsum tau ua hauv cov txheej txheem tsim tsev qiv ntawv.
