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Ib leeg- nucleus RNA Sequencing
Kev nce qib hauv tib lub xov tooj ntawm tes tuav thiab ib tus neeg siv tsev qiv ntawv tsim cov txheej txheem sib txuas nrog kev sib txuas ua haujlwm siab tso cai rau cov kev tshawb fawb gene ntawm cov xov tooj ntawm tes.Nws ua kom muaj kev sib sib zog nqus thiab ua tiav kev tshuaj xyuas ntawm cov cell complex, nyob rau hauv uas nws feem ntau zam qhov npog ntawm lawv cov heterogeneity los ntawm kev noj nruab nrab ntawm tag nrho cov hlwb.
Txawm li cas los xij, qee lub hlwb tsis tsim nyog los ua rau hauv ib leeg-cell ncua kev kawm, yog li lwm cov qauv kev npaj - xav tau kev rho tawm nucleus los ntawm cov ntaub so ntswg, uas yog, nucleus raug rho tawm ncaj qha los ntawm cov ntaub so ntswg lossis cell thiab npaj rau hauv ib qho kev ncua rau ib leeg- cell sequencing.
BMK muab 10 × Genomics ChromiumTM raws li ib leeg-cell RNA sequencing kev pabcuam.Cov kev pabcuam no tau siv dav hauv kev tshawb fawb txog kev tshawb fawb txog kab mob, xws li kev tiv thaiv kab mob sib txawv, qog heterogeneity, kev loj hlob ntawm cov ntaub so ntswg, thiab lwm yam.
Spatial transcriptome nti: 10 × Genomics
Platform: Illumina NovaSeq Platform
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BMKMANU S1000 Spatial Transcriptome
Spatial transcriptomics sawv ntawm qhov ua ntej ntawm kev tshawb fawb txog kev tsim kho tshiab, txhawb cov kws tshawb fawb kom delve rau hauv cov qauv ntawm cov noob caj noob ces hauv cov ntaub so ntswg thaum khaws cia lawv cov ntsiab lus spatial.Nyob nruab nrab ntawm ntau lub platform, BMKGene tau tsim BMKManu S1000 Spatial Transcriptome Chip, khav theebtxhim kho kev daws teeb meemntawm 5µM, ncav cuag subcellular ntau yam, thiab ua kom muajmulti-level daws teeb meem.Lub S1000 nti, uas muaj kwv yees li 2 lab qhov chaw, ntiav cov microwells txheej nrog cov hlaws dai nrog spatially barcoded capture probes.Lub tsev qiv ntawv cDNA, txhawb nqa nrog spatial barcodes, tau npaj los ntawm S1000 nti thiab tom qab ntawd tau ua tiav ntawm Illumina NovaSeq platform.Kev sib xyaw ua ke ntawm spatial barcoded qauv thiab UMIs ua kom qhov tseeb thiab tshwj xeeb ntawm cov ntaub ntawv tsim tawm.BMKManu S1000 nti tus cwj pwm tshwj xeeb yog nyob rau hauv nws txoj kev ua tau zoo, muab ntau theem kev daws teeb meem uas tuaj yeem raug kho kom zoo rau cov ntaub so ntswg sib txawv thiab theem ntawm kev nthuav dav.Qhov kev hloov pauv no tso lub nti ua qhov kev xaiv zoo tshaj plaws rau ntau yam kev tshawb fawb spatial transcriptomics, kom ntseeg tau meej qhov sib cais nrog cov suab nrov tsawg.
Siv BMKManu S1000 nti thiab lwm yam spatial transcriptomics thev naus laus zis, cov kws tshawb nrhiav tuaj yeem nkag siab zoo dua ntawm lub koom haum spatial ntawm cov hlwb thiab cov kev sib cuam tshuam ntawm cov molecular uas tshwm sim hauv cov ntaub so ntswg, muab kev nkag siab zoo rau cov txheej txheem hauv cov txheej txheem lom neeg hauv ntau thaj chaw, suav nrog oncology, neuroscience, kev loj hlob biology, immunology thiab botanical kev tshawb fawb.
Platform: BMKManu S1000 nti thiab Illumina NovaSeq
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10x Genomics Visium Spatial Transcriptome
Spatial transcriptomics yog ib qho kev siv thev naus laus zis uas tso cai rau cov kws tshawb fawb los tshawb xyuas cov qauv ntawm cov noob hauv cov ntaub so ntswg thaum khaws cia lawv cov ntsiab lus spatial.Ib lub platform muaj zog hauv qhov no yog 10x Genomics Visium ua ke nrog Illumina sequencing.Lub hauv paus ntsiab lus ntawm 10X Visium yog nyob ntawm ib qho tshwj xeeb nti nrog rau qhov chaw ntes uas tau muab tso rau hauv cov ntaub so ntswg.Qhov chaw ntes no muaj cov chaw barcoded, txhua qhov sib xws rau qhov chaw tshwj xeeb hauv cov ntaub so ntswg.Lub ntes RNA molecules los ntawm cov ntaub so ntswg yog tom qab ntawd sau nrog cov cim molecular identifiers (UMIs) thaum lub sijhawm rov qab cov txheej txheem.Cov chaw barcoded thiab UMIs pab kom meej spatial mapping thiab kom muaj nuj nqis ntawm cov noob qhia ntawm ib leeg-cell daws teeb meem.Kev sib xyaw ua ke ntawm spatial barcoded qauv thiab UMIs ua kom qhov tseeb thiab tshwj xeeb ntawm cov ntaub ntawv tsim tawm.Los ntawm kev siv thev naus laus zis Spatial Transcriptomics, cov kws tshawb fawb tuaj yeem nkag siab tob txog lub koom haum spatial ntawm cov hlwb thiab cov kev sib cuam tshuam ntawm cov molecular uas tshwm sim hauv cov ntaub so ntswg, muab kev nkag siab zoo rau cov txheej txheem hauv cov txheej txheem lom neeg hauv ntau qhov chaw, suav nrog oncology, neuroscience, kev loj hlob biology, immunology. , thiab kev tshawb fawb botanical.
Platform: 10X Genomics Visium thiab Illumina NovaSeq
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Full-Length mRNA Sequencing-Nanopore
Thaum NGS-raws li mRNA sequencing ua hauj lwm pab raws li ib tug ntau yam cuab yeej rau gene qhia quantification, nws tso siab rau ntawm luv luv nyeem txwv nws cov kev ua tau zoo nyob rau hauv complex transcriptomic analyses.Nanopore sequencing, ntawm qhov tod tes, ntiav cov thev naus laus zis nyeem ntev, ua kom muaj kev sib luag ntawm cov ntawv sau ntev mRNA.Txoj hauv kev no ua rau muaj kev tshawb nrhiav dav dav ntawm lwm txoj kev sib txuas, cov noob fusions, poly-adenylation, thiab qhov ntau ntawm mRNA isoforms.
Nanopore sequencing cia siab rau nanopore ib leeg-molecule hluav taws xob lub sijhawm tiag tiag.Kev coj los ntawm lub cev muaj zog proteins, ob-stranded DNA khi rau nanopore proteins embedded nyob rau hauv ib tug biofilm, unwinding raws li nws dhau los ntawm nanopore channel nyob rau hauv ib tug voltage sib txawv.Cov cim hluav taws xob tshwj xeeb tsim los ntawm cov hauv paus sib txawv ntawm DNA strand raug kuaj pom thiab cais tawm hauv lub sijhawm tiag tiag, ua kom muaj tseeb thiab txuas ntxiv nucleotide sequencing.Txoj hauv kev tshiab no kov yeej cov kev txwv luv luv thiab muab lub platform dynamic rau intricate genomic tsom xam, suav nrog txoj kev tshawb fawb transcriptomic.
Platform: Nanopore Promethion P48
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Tag nrho-ntev mRNA sequencing - PacBio
Thaum NGS-raws li mRNA sequencing yog ntau yam cuab yeej rau kev txheeb xyuas cov noob qhia, nws qhov kev cia siab ntawm kev nyeem luv luv txwv nws txoj kev siv hauv kev tshuaj ntsuam transcriptomic nyuaj.PacBio sequencing (Iso-Seq), ntawm qhov tod tes, ntiav cov thev naus laus zis nyeem ntev, ua kom muaj kev sib luag ntawm cov ntawv sau ntev mRNA.Txoj hauv kev no ua rau muaj kev tshawb nrhiav dav dav ntawm lwm txoj kev splicing, noob fusions thiab poly-adenylation txawm hais tias nws tsis yog qhov kev xaiv tseem ceeb rau kev nthuav qhia cov noob qoob loo, vim tias cov ntaub ntawv xav tau ntau heev.
PacBio sequencing thev naus laus zis tso siab rau ib leeg-molecule, real-time (SMRT) sequencing, muab qhov zoo sib xws hauv kev ntes tag nrho-ntev mRNA transcripts.Txoj hauv kev tshiab no suav nrog kev siv zero-mode waveguides (ZMWs), microfabricated qhov dej uas ua rau pom lub sijhawm tiag tiag ntawm DNA polymerase kev ua haujlwm thaum lub sijhawm ua ntu zus.Hauv cov ZMWs no, PacBio's DNA polymerase synthesizes a complementary strand of DNA, generating long reading that span the tag nrho ntawm mRNA transcripts.PacBio ua haujlwm hauv Circular Consensus sequencing (CCS) hom txhim kho qhov tseeb los ntawm kev rov ua kom zoo li qub molecule.Cov ntawv nyeem HiFi generated muaj qhov tseeb piv rau NGS, ntxiv rau kev nthuav dav thiab txhim khu kev txheeb xyuas ntawm cov yam ntxwv ntawm cov ntaub ntawv nyuaj.Platform: PacBio Sequel II
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Eukaryotic mRNA Sequencing-Illumina
mRNA sequencing txhawb nqa cov kev qhia dav dav ntawm txhua mRNA cov ntawv teev lus hauv cov hlwb hauv cov xwm txheej tshwj xeeb.Qhov kev siv thev naus laus zis no ua haujlwm zoo li cov cuab yeej muaj zog, nthuav tawm cov kab lus sib txawv ntawm cov noob caj noob ces, cov qauv noob, thiab cov txheej txheem molecular cuam tshuam nrog ntau yam txheej txheem lom neeg.Kev siv dav dav hauv kev tshawb fawb, kev kuaj mob, thiab kev tsim tshuaj, mRNA sequencing muab kev nkag siab rau hauv kev tsis sib haum xeeb ntawm cellular dynamics thiab kev tswj caj ces.
Platform: Illumina NovaSeq X
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Tsis siv neeg raws li mRNA Sequencing-Illumina
mRNA sequencing txhawb nqa cov kev qhia dav dav ntawm txhua mRNA cov ntawv teev lus hauv cov hlwb hauv cov xwm txheej tshwj xeeb.Qhov kev siv thev naus laus zis no ua haujlwm zoo li cov cuab yeej muaj zog, nthuav tawm cov kab lus sib txawv ntawm cov noob caj noob ces, cov qauv noob, thiab cov txheej txheem molecular cuam tshuam nrog ntau yam txheej txheem lom neeg.Kev siv dav dav hauv kev tshawb fawb, kev kuaj mob, thiab kev tsim tshuaj, mRNA sequencing muab kev nkag siab rau hauv kev tsis sib haum xeeb ntawm cellular dynamics thiab kev tswj caj ces.
Platform: Illumina NovaSeq X
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Ntev tsis-coding sequencing-Illumina
Ntev tsis-coding RNAs (lncRNAs) yog RNAs ntev dua 200 nucleotides uas muaj peev xwm coding tsawg thiab yog cov ntsiab lus tseem ceeb hauv cov tsis-coding RNA.Pom muaj nyob rau hauv cov nucleus thiab cytoplasm, cov RNAs ua lub luag haujlwm tseem ceeb hauv kev tswj hwm ntawm epigenetic, transcriptional, thiab post-transcriptional regulation, underscoring lawv qhov tseem ceeb hauv shaping cellular thiab molecular txheej txheem.LncRNA sequencing yog ib qho cuab yeej muaj zog hauv Cell sib txawv, Ontogenesis, thiab Tib neeg kab mob.
Platform: Illumina NovaSeq
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Me RNA sequencing-Illumina
Me me RNA (sRNA) molecules, feem ntau nyob rau hauv 200 nucleotides ntev, suav nrog microRNAs (miRNAs), me me cuam tshuam RNAs (siRNAs), thiab piwi-interacting RNAs (piRNAs).Ntawm cov no, miRNAs, nyob ib ncig ntawm 20-24 nucleotides ntev, tshwj xeeb tshaj yog tsim nyog rau lawv txoj haujlwm tseem ceeb ntawm kev tswj hwm hauv ntau cov txheej txheem ntawm tes.Nrog rau cov ntaub so ntswg tshwj xeeb thiab theem tshwj xeeb qhia cov qauv, miRNAs nthuav tawm kev txuag siab thoob plaws ntau hom.
Platform: Illumina NovaSeq
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CircRNA sequencing-Illumina
Circular RNA sequencing (circRNA-seq) yog txhawm rau txheeb xyuas thiab txheeb xyuas RNAs, chav kawm ntawm RNA molecules uas tsim cov voj voog kaw vim tsis yog-canonical splicing txheej xwm, muab cov RNA nrog kev ruaj ntseg.Thaum qee qhov circRNAs tau pom tias ua raws li microRNA sponges, sequestering microRNAs thiab tiv thaiv lawv los ntawm kev tswj lawv lub hom phiaj mRNAs, lwm cov circRNAs tuaj yeem cuam tshuam nrog cov proteins, hloov cov noob qhia, lossis muaj lub luag haujlwm hauv cov txheej txheem ntawm tes.circRNA qhia kev tsom xam muab kev nkag siab rau hauv kev tswj hwm lub luag haujlwm ntawm cov molecules thiab lawv qhov tseem ceeb hauv ntau cov txheej txheem ntawm tes, theem kev loj hlob, thiab cov kab mob, ua rau kom nkag siab tob txog qhov nyuaj ntawm RNA cov cai hauv cov ntsiab lus ntawm cov noob qhia.
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Tag nrho cov ntawv sau ua ke - Illumina
Tag nrho cov ntawv sau ua ke muaj kev qhia dav dav rau kev ua kom muaj ntau hom RNA molecules, suav nrog ob qho tib si coding (mRNA) thiab tsis-coding RNAs (lncRNA, circRNA, thiab miRNA).Cov txheej txheem no ntes tag nrho cov ntaub ntawv sau tseg ntawm cov hlwb tshwj xeeb ntawm ib lub sijhawm, tso cai rau kev nkag siab zoo ntawm cov txheej txheem cellular.Kuj tseem hu ua "tag nrho RNA sequencing," nws lub hom phiaj los nthuav tawm cov kev tswj hwm kev sib txuas lus sib txuas ntawm qib kev sau ntawv, ua kom muaj kev soj ntsuam tob xws li kev sib tw endogenous RNA (ceRNA) thiab sib koom RNA tsom xam.Qhov no yog thawj kauj ruam ntawm kev ua haujlwm ntawm tus cwj pwm, tshwj xeeb tshaj yog nyob rau hauv kev nthuav tawm cov kev tswj hwm kev sib txuas nrog kev sib cuam tshuam ntawm circRNA-miRNA-mRNA-based ceRNA.
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Prokaryotic mRNA sequencing
mRNA sequencing txhawb nqa cov kev qhia dav dav ntawm txhua mRNA cov ntawv teev lus hauv cov hlwb hauv cov xwm txheej tshwj xeeb.Qhov kev siv thev naus laus zis no ua haujlwm zoo li cov cuab yeej muaj zog, nthuav tawm cov kab lus sib txawv ntawm cov noob caj noob ces, cov qauv noob, thiab cov txheej txheem molecular cuam tshuam nrog ntau yam txheej txheem lom neeg.Kev siv dav dav hauv kev tshawb fawb, kev kuaj mob, thiab kev tsim tshuaj, mRNA sequencing muab kev nkag siab rau hauv kev tsis sib haum xeeb ntawm cellular dynamics thiab kev tswj caj ces.Peb cov qauv prokaryotic mRNA ua qauv yog tsim rau cov ntaub ntawv pov thawj prokaryotic, suav nrog rRNA depletion thiab kev npaj tsev qiv ntawv.
Platform: Illumina NovaSeq X
