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Ib leeg- nucleus RNA Sequencing
Kev nce qib hauv tib lub xov tooj ntawm tes tuav thiab ib tus neeg siv tsev qiv ntawv tsim cov txheej txheem sib txuas nrog kev sib txuas ua haujlwm siab tso cai rau cov kev tshawb fawb gene ntawm cov xov tooj ntawm tes.Nws ua kom muaj kev sib sib zog nqus thiab ua tiav kev tshuaj xyuas ntawm cov cell complex, nyob rau hauv uas nws feem ntau zam qhov npog ntawm lawv cov heterogeneity los ntawm kev noj nruab nrab ntawm tag nrho cov hlwb.
Txawm li cas los xij, qee lub hlwb tsis tsim nyog los ua rau hauv ib leeg-cell ncua kev kawm, yog li lwm cov qauv kev npaj - xav tau kev rho tawm nucleus los ntawm cov ntaub so ntswg, uas yog, nucleus raug rho tawm ncaj qha los ntawm cov ntaub so ntswg lossis cell thiab npaj rau hauv ib qho kev ncua rau ib leeg- cell sequencing.
BMK muab 10 × Genomics ChromiumTM raws li ib leeg-cell RNA sequencing kev pabcuam.Cov kev pabcuam no tau siv dav hauv kev tshawb fawb txog kev tshawb fawb txog kab mob, xws li kev tiv thaiv kab mob sib txawv, qog heterogeneity, kev loj hlob ntawm cov ntaub so ntswg, thiab lwm yam.
Spatial transcriptome nti: 10 × Genomics
Platform: Illumina NovaSeq Platform
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BMKMANU S1000 Spatial Transcriptome
Spatial transcriptomics sawv ntawm qhov ua ntej ntawm kev tshawb fawb txog kev tsim kho tshiab, txhawb cov kws tshawb fawb kom delve rau hauv cov qauv ntawm cov noob caj noob ces hauv cov ntaub so ntswg thaum khaws cia lawv cov ntsiab lus spatial.Nyob nruab nrab ntawm ntau lub platform, BMKGene tau tsim BMKManu S1000 Spatial Transcriptome Chip, khav theebtxhim kho kev daws teeb meemntawm 5µM, ncav cuag subcellular ntau yam, thiab ua kom muajmulti-level daws teeb meem.Lub S1000 nti, uas muaj kwv yees li 2 lab qhov chaw, ntiav cov microwells txheej nrog cov hlaws dai nrog spatially barcoded capture probes.Lub tsev qiv ntawv cDNA, txhawb nqa nrog spatial barcodes, tau npaj los ntawm S1000 nti thiab tom qab ntawd tau ua tiav ntawm Illumina NovaSeq platform.Kev sib xyaw ua ke ntawm spatial barcoded qauv thiab UMIs ua kom qhov tseeb thiab tshwj xeeb ntawm cov ntaub ntawv tsim tawm.BMKManu S1000 nti tus cwj pwm tshwj xeeb yog nyob rau hauv nws txoj kev ua tau zoo, muab ntau theem kev daws teeb meem uas tuaj yeem raug kho kom zoo rau cov ntaub so ntswg sib txawv thiab theem ntawm kev nthuav dav.Qhov kev hloov pauv no tso lub nti ua qhov kev xaiv zoo tshaj plaws rau ntau yam kev tshawb fawb spatial transcriptomics, kom ntseeg tau meej qhov sib cais nrog cov suab nrov tsawg.
Siv BMKManu S1000 nti thiab lwm yam spatial transcriptomics thev naus laus zis, cov kws tshawb nrhiav tuaj yeem nkag siab zoo dua ntawm lub koom haum spatial ntawm cov hlwb thiab cov kev sib cuam tshuam ntawm cov molecular uas tshwm sim hauv cov ntaub so ntswg, muab kev nkag siab zoo rau cov txheej txheem hauv cov txheej txheem lom neeg hauv ntau thaj chaw, suav nrog oncology, neuroscience, kev loj hlob biology, immunology thiab botanical kev tshawb fawb.
Platform: BMKManu S1000 nti thiab Illumina NovaSeq
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10x Genomics Visium Spatial Transcriptome
Spatial transcriptomics yog ib qho kev siv thev naus laus zis uas tso cai rau cov kws tshawb fawb los tshawb xyuas cov qauv ntawm cov noob hauv cov ntaub so ntswg thaum khaws cia lawv cov ntsiab lus spatial.Ib lub platform muaj zog hauv qhov no yog 10x Genomics Visium ua ke nrog Illumina sequencing.Lub hauv paus ntsiab lus ntawm 10X Visium yog nyob ntawm ib qho tshwj xeeb nti nrog rau qhov chaw ntes uas tau muab tso rau hauv cov ntaub so ntswg.Qhov chaw ntes no muaj cov chaw barcoded, txhua qhov sib xws rau qhov chaw tshwj xeeb hauv cov ntaub so ntswg.Lub ntes RNA molecules los ntawm cov ntaub so ntswg yog tom qab ntawd sau nrog cov cim molecular identifiers (UMIs) thaum lub sijhawm rov qab cov txheej txheem.Cov chaw barcoded thiab UMIs pab kom meej spatial mapping thiab kom muaj nuj nqis ntawm cov noob qhia ntawm ib leeg-cell daws teeb meem.Kev sib xyaw ua ke ntawm spatial barcoded qauv thiab UMIs ua kom qhov tseeb thiab tshwj xeeb ntawm cov ntaub ntawv tsim tawm.Los ntawm kev siv thev naus laus zis Spatial Transcriptomics, cov kws tshawb fawb tuaj yeem nkag siab tob txog lub koom haum spatial ntawm cov hlwb thiab cov kev sib cuam tshuam ntawm cov molecular uas tshwm sim hauv cov ntaub so ntswg, muab kev nkag siab zoo rau cov txheej txheem hauv cov txheej txheem lom neeg hauv ntau qhov chaw, suav nrog oncology, neuroscience, kev loj hlob biology, immunology. , thiab kev tshawb fawb botanical.
Platform: 10X Genomics Visium thiab Illumina NovaSeq
