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Illumina thiab BGI
Illumina sequencing thev naus laus zis, raws li Sequencing los ntawm Synthesis (SBS), yog lub ntiaj teb embraced NGS innovation, lub luag hauj lwm rau generating tshaj 90% ntawm lub ntiaj teb no sequencing cov ntaub ntawv.Lub hauv paus ntsiab lus ntawm SBS suav nrog cov duab fluorescently sau cov ntawv thim rov qab raws li txhua qhov dNTP tau ntxiv, thiab tom qab ntawd tau txiav kom tso cai rau kev koom ua ke ntawm lub hauv paus tom ntej.Nrog rau tag nrho plaub qhov thim rov qab-txhim kho dNTPs tam sim no nyob rau hauv txhua lub voj voog sib luag, kev sib tw ntuj txo qis kev sib koom ua ke.Qhov kev siv thev naus laus zis no txhawb nqa ob qho tib si nyeem ib leeg thiab ua ke-kawg cov tsev qiv ntawv, ua rau ntau yam kev siv genomic.Illumina sequencing's high-throughput peev xwm thiab precision qhov chaw nws yog lub hauv paus hauv kev tshawb fawb genomics, txhawb cov kws tshawb fawb los daws qhov tsis sib xws ntawm cov genomes nrog cov ntsiab lus tsis sib xws thiab kev ua haujlwm zoo.
DNBSEQ, tsim los ntawm BGI, yog lwm qhov tshiab NGS thev naus laus zis uas tau tswj hwm kom txo qis cov nqi sib txuas ntxiv thiab nce kev nce qib.Kev npaj ntawm DNBSEQ cov tsev qiv ntawv suav nrog DNA fragmentation, kev npaj ntawm ssDNA thiab dov lub voj voog kom tau txais cov DNA nanoballs (DNB).Cov no yog tom qab ntawd thauj mus rau qhov chaw ruaj khov thiab tom qab sib txuas los ntawm combinatorial Probe-Anchor Synthesis (cPAS).
Peb lub tsev qiv ntawv ua ntej ua tiav cov kev pabcuam pab txhawb cov neeg siv khoom hauv kev npaj lawv cov tsev qiv ntawv sib txuas los ntawm ntau qhov chaw (mRNA, tag nrho genome, amplicon, thiab lwm yam).Tom qab ntawd, cov tsev qiv ntawv no tuaj yeem raug xa mus rau peb cov chaw sib txuas ua ke rau kev tswj xyuas zoo thiab sib txuas hauv Illumina lossis BGI platforms.
