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Metagenomic Sequencing -NGS
Metagenome yog hais txog kev sau tag nrho cov khoom siv caj ces ntawm cov zej zog sib xyaw ntawm cov kab mob, xws li ib puag ncig metagenome, tib neeg metagenome, thiab lwm yam. Nws muaj cov genomes ntawm ob qho tib si cultivatable thiab uncultivatable microorganisms.Metagenomic sequencing yog cov cuab yeej molecular siv los txheeb xyuas cov khoom sib xyaw genomic muab rho tawm los ntawm ib puag ncig cov qauv, uas muab cov ncauj lus kom ntxaws txog hom kev sib txawv thiab kev nplua nuj, cov qauv pej xeem, kev sib raug zoo ntawm phylogenetic, cov noob caj noob ces thiab kev sib raug zoo nrog ib puag ncig.
Platform:Illumina NovaSeq Platform
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Metagenomic Sequencing-Nanopore
Metagenomics yog cov cuab yeej molecular siv los tshuaj xyuas cov khoom sib xyaw genomic muab rho tawm los ntawm ib puag ncig cov qauv, uas muab cov ncauj lus kom ntxaws txog hom kev sib txawv thiab kev nplua nuj, cov qauv pejxeem, kev sib raug zoo ntawm cov noob caj noob ces thiab kev sib raug zoo nrog ib puag ncig, thiab lwm yam. Nanopore sequencing platforms tau qhia tsis ntev los no. rau kev tshawb fawb metanomic.Nws qhov kev ua tau zoo tshaj plaws hauv kev nyeem ntev tau txhim kho cov dej ntws ntsuas metagenomic, tshwj xeeb tshaj yog kev sib dhos metagenome.Siv qhov zoo ntawm kev nyeem-ntev, Nanopore-based metaagenomic txoj kev tshawb no muaj peev xwm ua tiav kev sib dhos txuas ntxiv piv nrog cov phom phom metagenomics.Nws tau raug luam tawm tias Nanopore-based metagenomics tau ua tiav tiav thiab kaw cov kab mob genomes los ntawm microbiomes (Moss, EL, et. al,Ntuj Biotech, 2020)
Platform:Nanopor PromethION P48
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16S/18S/ITS Amplicon Sequencing-PacBio
Lub subunit ntawm 16S thiab 18S rRNA uas muaj ob qho tib si kev txuag thiab cov cheeb tsam hyper-variable yog ib qho zoo meej molecular ntiv tes rau kev txheeb xyuas cov kab mob prokaryotic thiab eukaryotic.Ua kom zoo dua ntawm kev sib txuas, cov amplicons tuaj yeem raug tsom raws li qhov chaw khaws cia thiab cov cheeb tsam hyper-variable tuaj yeem ua tus yam ntxwv zoo rau kev txheeb xyuas cov kab mob microbial uas ua rau muaj kev tshawb fawb txog kev tsom xam microbial ntau haiv neeg, taxonomy, phylogeny, thiab lwm yam. Ib leeg-molecule real-time (SMRT ) sequencing ntawm PacBio platform ua rau kom tau txais kev nyeem ntawv ntev heev, uas tuaj yeem npog tag nrho-ntev amplicons (kwv yees li 1.5 Kb).Kev nthuav dav ntawm cov noob caj noob ces zoo heev txhim kho qhov kev daws teeb meem hauv hom lus piav qhia hauv cov kab mob lossis cov kab mob fungi.
Platform:PacBio Sequel II
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16S/18S/ITS Amplicon Sequencing-NGS
16S / 18S / ITS amplicon sequencing aims ntawm kev nthuav tawm phylogeny, taxonomy, thiab tsiaj abundance nyob rau hauv ib tug microbial zej zog los ntawm kev soj ntsuam PCR cov khoom ntawm tsev tu cov noob caj noob ces cov cim uas muaj ob qho tib si sib tham thiab hypervariable qhov chaw.Kev taw qhia ntawm cov ntiv tes zoo meej molecular los ntawm Woeses et al, (1977) txhawb nqa kev sib cais-dawb microbiome profiling.Sequencing ntawm 16S (cov kab mob), 18S (fungi) thiab Internal transcribed spacer (ITS, fungi) tso cai rau kev txheeb xyuas ntawm ob qho tib si ntau hom nrog rau cov hom tsis tshua muaj thiab tsis muaj npe.Cov cuab yeej siv no tau dhau los ua cov cuab yeej siv dav thiab tseem ceeb hauv kev txheeb xyuas qhov sib txawv ntawm cov kab mob microbial nyob rau hauv ntau qhov chaw, xws li tib neeg lub qhov ncauj, txoj hnyuv, quav, thiab lwm yam.
Platform:Illumina NovaSeq Platform
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Cov kab mob thiab cov kab mob fungal tag nrho genome Re-Sequencing
Cov kab mob thiab cov kab mob fungal tag nrho cov genome re-sequencing yog ib qho cuab yeej tseem ceeb los ua kom tiav cov genomes ntawm cov kab mob paub cov kab mob thiab cov fungi, nrog rau kev sib piv ntau cov genomes los yog daim ntawv qhia genomes ntawm cov kab mob tshiab.Nws yog ib qho tseem ceeb heev kom ua raws li tag nrho cov genomes ntawm cov kab mob thiab cov fungi txhawm rau tsim kom raug cov genomes siv, ua cov cim microbial thiab lwm yam kev tshawb fawb genome sib piv.
Platform: Illumina NovaSeq Platform
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Metatranscriptome Sequencing
Metatranscriptome sequencing indentifies gene expression of microbes (ob leeg eukaryotes thiab prokaryotes) nyob rau hauv tej yam ntuj tso ib puag ncig (xws li av, dej, hiav txwv, quav, thiab plab.). ntawm hom, kev ua kom muaj txiaj ntsig zoo ntawm kev qhia cov noob sib txawv, thiab lwm yam.
Platform: Illumina NovaSeq Platform
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Cov kab mob fungal
Biomarker Technologies muab kev tshawb fawb genome, zoo genome thiab pene-ua tiav genome ntawm fungal nyob ntawm lub hom phiaj tshawb fawb tshwj xeeb.genome sequencing, sib dhos thiab kev ua haujlwm annotation tuaj yeem ua tiav los ntawm kev sib txuas ua ke Next-tiam sequencing + Peb tiam sequencing kom ua tiav qib siab genome los ua ke.Hi-C thev naus laus zis kuj tseem tuaj yeem ua haujlwm los pab txhawb genome los ua ke ntawm qib chromosome.
Platform:PacBio Sequel II
Nanopor PromethION P48
Illumina NovaSeq Platform
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Cov kab mob ua tiav Genome
Biomarker Technologies muab kev pabcuam ua ntu zus ntawm kev tsim cov genome ntawm cov kab mob uas tsis muaj qhov sib txawv.Lub luag haujlwm tseem ceeb ntawm cov kab mob ua tiav kev tsim kho genome suav nrog kev sib txuas thib peb, kev sib dhos, cov lus piav qhia ua haujlwm thiab kev tshawb xyuas bioinformatic siab ua tiav cov hom phiaj kev tshawb fawb tshwj xeeb.Kev nthuav qhia ntau dua ntawm cov kab mob genome txhawb kev nthuav tawm cov txheej txheem tseem ceeb hauv qab lawv cov txheej txheem lom neeg, uas tuaj yeem muab cov ntaub ntawv tseem ceeb rau kev tshawb fawb genomic hauv ntau hom eukaryotic.
Platform:Nanopore PromethION P48 + Illumina NovaSeq Platform
PacBio Sequel II
