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PacBio-Full-ntev 16S / 18S / ITS Amplicon Sequence
Amplicon (16S / 18S / ITS) platform yog tsim los nrog ntau xyoo ntawm kev paub nyob rau hauv microbial ntau haiv neeg qhov kev tsom xam, uas muaj cov qauv kev tshuaj ntsuam xyuas thiab kev txheeb xyuas tus kheej: kev tshuaj xyuas yooj yim npog cov ntsiab lus tseem ceeb ntawm kev tshawb fawb microbial tam sim no, cov ntsiab lus tsom xam yog nplua nuj thiab ua tiav, thiab kev soj ntsuam cov txiaj ntsig tau nthuav tawm hauv daim ntawv qhia txog qhov project;Cov ntsiab lus ntawm kev txheeb xyuas tus kheej muaj ntau yam.Cov qauv tuaj yeem xaiv tau thiab tsis tuaj yeem teeb tsa tau yooj yim raws li daim ntawv qhia kev txheeb xyuas thiab kev tshawb fawb lub hom phiaj, kom paub txog cov kev xav tau ntawm tus kheej.Windows operating system, yooj yim thiab ceev.
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PacBio-Full-length Transcriptome (Tsis Siv Neeg)
Noj Pacific Biosciences (PacBio) Isoform sequencing cov ntaub ntawv raws li kev tawm tswv yim, App no muaj peev xwm txheeb xyuas cov ntaub ntawv sau ua ntu zus (tsis muaj kev sib dhos).Los ntawm daim ntawv qhia tag nrho-ntev ib ntus tawm tsam kev siv genome, cov ntawv sau tuaj yeem ua kom zoo dua los ntawm cov noob caj noob ces, cov ntawv sau tseg, cov cheeb tsam coding, thiab lwm yam. Nyob rau hauv rooj plaub no, kev txheeb xyuas qhov tseeb dua ntawm mRNA cov qauv, xws li lwm txoj kev sib txuas, thiab lwm yam, tuaj yeem ua tiav.Kev sib koom ua ke nrog NGS cov ntaub ntawv teev cov ntaub ntawv sib txuas ua kom muaj ntau cov lus piav qhia thiab kev txheeb xyuas qhov tseeb ntau dua hauv kev qhia ntawm qib ntawv sau, uas feem ntau tau txais txiaj ntsig zoo hauv nqe lus sib txawv thiab kev tshuaj xyuas ua haujlwm.
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Cov cuab yeej
BMKCloud yog ib qho chaw ua haujlwm bioinformatic uas muab ib qho kev daws teeb meem rau cov kev pabcuam genomic, uas tau txais kev ntseeg siab los ntawm cov kws tshawb fawb hauv ntau qhov chaw suav nrog kev kho mob, kev ua liaj ua teb, ib puag ncig, thiab lwm yam. BMKCloud tau cog lus los muab kev pabcuam kev sib koom ua ke, txhim khu kev qha thiab muaj txiaj ntsig nrog rau bioinformatic tsom xam platforms thiab cov cuab yeej. , suav cov peev txheej, cov ntaub ntawv pej xeem, cov chav kawm bioinformtic online, thiab lwm yam BMKCloud muaj ntau yam nquag siv bioinformatic cov cuab yeej xws li cov gene annotation, evolutionary genetic cuab yeej, ncRNA, cov ntaub ntawv zoo tswj, sib dhos, sib dhos, cov ntaub ntawv rho tawm, hloov pauv, txheeb cais, daim duab generator, ib ntus tsom xam, thiab lwm yam.
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me RNA
Me RNAs yog hom luv luv tsis-coding RNA nrog qhov nruab nrab ntev ntawm 18-30 nt, suav nrog miRNA, siRNA thiab piRNA.Cov RNAs me me no tau raug tshaj tawm tias muaj kev koom tes hauv ntau yam kev lom neeg xws li mRNA degradation, translation inhibition, heterochromatin tsim, thiab lwm yam. SmallRNA sequencing tsom xam tau siv dav hauv kev tshawb fawb txog tsiaj / cog kev loj hlob, kab mob, kab mob, thiab lwm yam. sequencing tsom xam platform muaj cov txheej txheem tsom xam thiab qib siab cov ntaub ntawv mining.Nyob rau hauv lub hauv paus ntawm RNA-seq cov ntaub ntawv, kev soj ntsuam tus qauv tuaj yeem ua tiav miRNA kev txheeb xyuas thiab kev kwv yees, miRNA lub hom phiaj kev kwv yees, annotation thiab kev tsom xam.Kev soj ntsuam qib siab ua rau muaj kev hloov kho miRNA tshawb nrhiav thiab rho tawm, Venn daim duab tiam, miRNA thiab lub hom phiaj gene network tsim.
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NWS-WGS (Illumina/BGI)
NGS-WGS yog tag nrho genome re-sequencing tsom xam platform, uas yog tsim los ntawm lub hauv paus ntawm kev nplua nuj nyob rau hauv Biomarker Technologies.Qhov yooj yim-rau-siv platform no tso cai rau kev xa tawm sai ntawm kev sib koom ua ke ntawm kev ua haujlwm los ntawm kev teeb tsa ob peb yam yooj yim, uas haum rau DNA sequencing cov ntaub ntawv tsim los ntawm ob qho tib si Illumina platform thiab BGI sequencing platform.Lub platform no yog siv los ntawm kev ua haujlwm siab ntawm cov neeg siv computer, uas txhawb nqa cov ntaub ntawv txheeb xyuas cov ntaub ntawv muaj txiaj ntsig zoo hauv lub sijhawm txwv.Tus kheej cov ntaub ntawv tsuas yog muaj nyob rau hauv lub hauv paus ntawm kev soj ntsuam tus qauv, nrog rau cov lus nug hloov pauv, PCR primer tsim, thiab lwm yam.
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mRNA (Reference)
Transcriptome yog qhov txuas ntawm cov ntaub ntawv genomic genetic thiab proteome ntawm kev ua haujlwm lom neeg.Transcriptional Level regulation yog qhov tseem ceeb tshaj plaws thiab txoj kev kawm dav tshaj plaws ntawm cov kab mob.Transcriptome sequencing tuaj yeem ua raws cov ntawv sau tseg ntawm txhua lub sijhawm lossis nyob rau hauv ib qho xwm txheej, nrog rau kev daws teeb meem raug rau ib qho nucleotide.Nws tuaj yeem cuam tshuam txog qib ntawm cov noob hloov pauv, ib txhij txheeb xyuas thiab txheeb xyuas cov ntaub ntawv tsis tshua muaj thiab ib txwm muaj, thiab muab cov ntaub ntawv tsim nyog ntawm piv txwv cov ntawv sau tseg.
Tam sim no, transcriptome sequencing thev naus laus zis tau siv dav hauv agronomy, tshuaj thiab lwm yam kev tshawb fawb, suav nrog kev tswj hwm tsiaj thiab nroj tsuag, kev hloov pauv ib puag ncig, kev tiv thaiv kab mob, cov noob hauv cheeb tsam, hom kab mob evolution thiab qog thiab kuaj kab mob caj ces.
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Metagenomics (NGS)
Qhov kev tshuaj ntsuam xyuas platform no yog tsim los rau kev tshawb xyuas cov ntaub ntawv phom phom metagenomic raws li kev paub ntau xyoo.Nws muaj kev sib xyaw ua haujlwm uas muaj ntau yam kev xav tau metagenomics tsom xam suav nrog kev ua cov ntaub ntawv, hom kev tshawb fawb qib, kev tshawb fawb txog qib kev ua haujlwm, metagenome binning, thiab lwm yam. Tsis tas li ntawd, cov cuab yeej kho cov ntaub ntawv tsuas yog muaj nyob rau hauv cov qauv kev soj ntsuam kev ua haujlwm, suav nrog cov noob thiab hom lus nug. , parameter teeb tsa, tus kheej daim duab tsim, thiab lwm yam.
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LncRNA
Ntev tsis-coding RNAs (lncRNA) yog ib hom ntawv sau nrog ntev dua 200 nt, uas tsis tuaj yeem sau cov proteins.Cov ntaub ntawv pov thawj qhia tau tias feem ntau ntawm lncRNAs feem ntau yuav ua haujlwm.Cov thev naus laus zis ntau dhau los thiab cov cuab yeej tshuaj xyuas bioinformatic pab txhawb peb kom nthuav tawm lncRNA cov kab ke thiab muab cov ntaub ntawv qhia tau zoo dua thiab coj peb mus nrhiav lncRNAs nrog cov haujlwm tseem ceeb ntawm kev tswj hwm.BMKCloud zoo siab los muab peb cov neeg siv khoom lncRNA sequencing tsom xam platform kom ua tiav ceev, txhim khu kev qha thiab hloov pauv lncRNA tsom xam.
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GWAS
Genome-wide Association txoj kev tshawb fawb (GWAS) lub hom phiaj ntawm kev txheeb xyuas cov noob caj noob ces (genotype) uas cuam tshuam nrog cov yam ntxwv tshwj xeeb (phenotype).GWA cov kev tshawb fawb tshawb xyuas cov cim caj ces hla tag nrho cov genome ntawm cov neeg coob coob thiab kwv yees cov koom haum genotype-phenotype los ntawm kev txheeb xyuas cov lej ntawm cov pej xeem.Tag nrho-genome resequencing tuaj yeem nrhiav pom txhua yam kab mob sib txawv.Kev sib txuas nrog cov ntaub ntawv phenotypic, GWAS tuaj yeem ua tiav los txheeb xyuas cov phenotype ntsig txog SNPs, QTLs thiab cov noob neeg sib tw, uas txhawb nqa cov tsiaj niaj hnub / cog qoob loo.SLAF yog tus kheej-tsim simplified genome sequencing lub tswv yim, uas pom genome-wide faib cov cim, SNP.Cov SNPs no, raws li cov cim caj ces molecular, tuaj yeem ua tiav rau cov kev tshawb fawb koom nrog nrog cov hom phiaj.Nws yog ib qho txiaj ntsig zoo hauv kev txheeb xyuas cov yam ntxwv nyuaj uas cuam tshuam txog kev hloov pauv caj ces.
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Nanopore Full-length transcriptomics
Cov txheej txheem nyuaj thiab sib txawv ntawm cov isoforms hauv cov kab mob yog cov txheej txheem tseem ceeb ntawm cov noob caj noob ces rau kev tswj cov noob qhia thiab ntau hom protein.Kev txheeb xyuas qhov tseeb ntawm cov qauv sau ntawv yog lub hauv paus rau kev kawm tob txog cov qauv kev cai ntawm cov noob caj noob ces.Nanopore sequencing platform tau ua tiav qhov kev kawm transcriptomic rau isoform-theem.Qhov kev tsom xam platform no yog tsim los tshuaj xyuas RNA-Seq cov ntaub ntawv tsim tawm ntawm Nanopore platform raws li kev siv genome, uas ua tiav cov kev soj ntsuam zoo thiab ntau npaum li cas hauv qib gene thiab cov ntawv sau tseg.
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circ-RNA
Circular RNA (circRNA) yog hom tsis-coding RNA, uas tsis ntev los no tau pom los ua lub luag haujlwm tseem ceeb hauv kev tswj hwm kev koom tes hauv kev tsim, kev tiv thaiv ib puag ncig, thiab lwm yam txawv ntawm linear RNA molecules, xws li mRNA, lncRNA, 3′ thiab 5′ qhov kawg ntawm circRNA tau koom ua ke los tsim ib lub voj voog, uas txuag lawv los ntawm kev zom cov exonuclease thiab ruaj khov dua li feem ntau ntawm cov kab RNA.CircRNA tau pom tias muaj ntau lub luag haujlwm hauv kev tswj cov noob caj noob ces.CircRNA tuaj yeem ua tau raws li ceRNA, uas khi miRNA sib tw, hu ua miRNA daim txhuam cev.CircRNA sequencing tsom xam platform txhawb circRNA qauv thiab qhia kev tsom xam, lub hom phiaj kwv yees thiab kev sib koom ua ke nrog lwm hom RNA molecules
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BSA
Bulked Segregant Analysis platform muaj cov txheej txheem kev soj ntsuam ib-kauj ruam thiab kev soj ntsuam siab heev nrog rau kev teeb tsa kev cai.BSA yog cov txheej txheem ua haujlwm sai sai txheeb xyuas cov phenotype txuam nrog cov cim caj ces.Lub luag haujlwm tseem ceeb ntawm BSA muaj: 1. xaiv ob pawg ntawm cov tib neeg uas muaj kev tawm tsam phenotypes;2. muab cov DNA, RNA lossis SLAF-seq (Tsim los ntawm Biomarker) ntawm txhua tus neeg los ua ob feem ntawm DNA;3. txheeb xyuas qhov sib txawv ntawm cov genome los yog nyob nruab nrab ntawm, 4. kwv yees cov neeg sib tw txuas cov cheeb tsam los ntawm ED thiab SNP-index algorithm;5. Kev soj ntsuam ua haujlwm thiab ua kom muaj txiaj ntsig zoo ntawm cov noob hauv cheeb tsam tus neeg sib tw, thiab lwm yam. Ntau cov mining siab heev hauv cov ntaub ntawv suav nrog kev tshuaj ntsuam genetic marker thiab primer design kuj muaj.
