GENOME EVOLUTION
Genome De Novo
Kev sib dhos | Kev txiav txim siab poj niam txiv neej Lub luag haujlwm tag nrho thiab cov kev pabcuam bioinformatic ib nrab tau muab los ntawm Biomarker Techonologies.
Abstract
“Lub cim phenotype ntawm seadragons suav nrog nplooj zoo li appendages, lub qhov ncauj tsis muaj hniav, thiab txiv neej cev xeeb tub nrog rau kev tsim cov qe fertilized rau ntawm qhov qhib "brood patch."Peb de novo-sequenced txiv neej thiab poj niam genomes ntawm cov seadragon (Phyllopteryx taeniolatus) thiab nws cov tsiaj muaj feem xyuam nrog, cov ntses ntses ntses (Syngnathoides biaculeatus).Kev hloov pauv cov ntaub ntawv los ntawm kev hloov pauv tshiab, cov nplooj zoo li appendages, qhia tau hais tias ib txheej ntawm cov noob feem ntau koom nrog hauv kev loj hlob fin tau koom ua ke nrog rau kev txhawb nqa ntawm tran scripts rau cov ntaub so ntswg kho thiab tiv thaiv kab mob.Lub zebrafish mutants rau scpp5, uas ploj hauv tag nrho cov syngnathids, tau pom tias tsis muaj lossis muaj cov hniav deformed pharyngeal, txhawb cov kev xav tias kev poob ntawm scpp5 tau ua rau poob ntawm cov hniav hauv syngnathids.Ib qho kev sib deev-kev txiav txim siab qhov chaw encoding tus txiv neej tshwj xeeb amhr2y noob sib koom los ntawm cov seadragon thiab alligator pipefish tau txheeb xyuas. ”
Cov ntaub ntawv thiab cov txheej txheem:
Khoom siv
Fpeb cov seadragon (P. taeniolatus) thiab ob tug ntses pas ntses (S. biaculetus) cov qauv.Ob tug seadragon (ib tug txiv neej thiab ib tug poj niam) thiab ob tug alligator pipefish (ib tug txiv neej thiab ib tug poj niam) cov tib neeg tau siv rau tag nrho-genome sequencing, thiab ob tug lwm tus txiv neej seadragon tib neeg tau siv rau genome resequencing.
Genome Sequency Strategy
270/350 bp tsev qiv ntawv (Hiseq 2500) + Pacbio 20Kb (Sequel) los yog Nanopore 30 Kb (MinION) + Hi-C (NovaSeq 6000).sequencing, thiab ob tug lwm tus txiv neej seadragon tib neeg tau siv rau genome resequencing.
Transscriptome Sequencing
Flos yog tej seadragon, hlwb, qhov muag, gill, siab, plawv, raum, mesentery, hnyuv, nqaij, fin, tawv nqaij, nplooj zoo li appendages, testis, thiab zes qe menyuam.
Genome de novo los ua ke lub tswv yim
Canu (Correction) + WTDBG2 (Assembly) + Pilon(Polish) + Lachesis (Hi-C).
Cov ntsiab lus tseem ceeb
Daim duab 1. Cov yam ntxwv tseem ceeb ntawm cov seadragon (P. taeniolatus) thiab cov ntses ntses ntses (S. biaculeatus) thiab lawv txoj haujlwm phylogenetic.
Fig. 2. Morphology thiab genetic pleev ntawm nplooj zoo li appendages nyob rau hauv cov seadragon (P. taeniolatus).
Fig. 3. Putative sex determination gene in the common seadragon (P. taeniolatus) and the alligator pipefish (S. biaculeatus).
Fig. 4. Pharyngeal tooth phenotypes in zebrafish scpp5 homozygous mutants.
Post lub sij hawm: Jan-08-2022