Nanopore-based Full-length Transcriptome Sequencing
Nanopore sequencing distinguishes nws tus kheej los ntawm lwm cov sequencing platforms, nyob rau hauv hais tias cov nucleotides nyeem ncaj qha tsis muaj DNA synthesis thiab generates ntev nyeem ntawm kaum ntawm kilobases.Qhov no txhawb nqa ncaj qha nyeem-tawm ntawm cov ntawv sau ntev ntev thiab daws cov teeb meem hauv kev tshawb fawb transcriptomic.
√ Tsawg ib ntus tshwj xeeb kev tsis ncaj ncees
√ Tag nrho-ntev nyeem ntawv ntawm cDNA rau kev tshawb fawb cov qauv noob
√ Cov ntaub ntawv tsawg dua yuav tsum tau them rau tib tus lej ntawm cov ntawv sau tseg
√ Kev txheeb xyuas ntau yam isoforms ib lub noob
√ Kev nthuav qhia kom muaj nuj nqis ntawm isoform-theem
Hom Bioinformatics Pipeline
Cov xwm txheej zoo kawg nkaus nrog Biomarker Technologies
1. Kev txheeb xyuas qhov sib piv ntawm cov ntawv sau ntev ntev qhia Gnetum luofuense qia kev loj hlob zoo
Phau ntawv Journal: Frontiers in Genetics
Published: Mar. 2021
Ntsiab lus: MinION |Lwm txoj kev splicing |APA |lncRNA |Differentially qhia transcripts
Nyeem Cov ntawv nyeem puv
2. Unidirectional txav ntawm me RNAs los ntawm tua mus rau cov hauv paus hniav hauv interspecific heterografts
Phau ntawv: Nature Plants
Luam tawm: Lub Ib Hlis 2021
Keywords: Nanopore |Illumina |tag nrho-ntev mobile mRNA nrhiav kom tau
3. Tag nrho-ntev transcriptome tsom xam ntawm asparagus cov hauv paus hniav nthuav tawm lub molecular mechanism ntawm ntsev kam rau ua los ntawm arbuscular mycorrhizal fungi
Phau ntawv Journal: Environmental and Experimental Botany
Luam tawm: Lub Ib Hlis 2021
Ntsiab lus: MinION |Cov ntawv sau sib txawv |Transcription yam |Lwm txoj kev splicing
4. Cov yam ntxwv ntawm lub cev hloov pauv thiab cov ntawv sau ntev ntev ntawm Rose (Rosa chinensis) Cov hauv paus hniav thiab nplooj nyob rau hauv teb rau kev ntxhov siab.
Phau ntawv Journal: Plant and Cell Physiology
Published: Oct. 2020
Ntsiab lus: PromethION |Cov ntawv sau sib txawv |Muaj nuj nqi kwv yees |Spliced isoforms |TF thiab lncRNA
5. Kev soj ntsuam thiab kev sib piv ntawm PacBio thiab nanopore-based RNA sequencing ntawm Arabidopsis transcriptome
Phau ntawv Journal: Plant Method
Tshaj tawm: Plaub Hlis 2020
Ntsiab lus: GridION |PromethION |PacBio Sequel |Illumina NovaSeq |genome alignment |Cov ntaub ntawv pov thawj |Lwm txoj kev splicing |SSR |LncRNA |Isoform quantification
Xov xwm thiab qhov tseem ceeb Lub hom phiaj ntawm kev sib koom cov xwm txheej ua tiav tshiab nrog Biomarker Technologies, khaws cov kev tshawb fawb tshiab thiab cov txuj ci tseem ceeb siv thaum kawm.
Post lub sij hawm: Jan-08-2022