GWAS
Lub npe: Tag nrho-genome resequencing qhia Brassica napus keeb kwm thiab caj ces loci koom nrog nws txoj kev txhim kho
Phau ntawv Journal: Nature Communications
NWS |WGS |Rov ua dua |GWAS |Cov ntawv sau tseg |RNAseq |Brassica napus |Evolution |Kev nyob hauv tsev
Hauv txoj kev tshawb no, Biomarker Technologies tau muab Kev Pabcuam ntawm NGS sequencing, nrog rau kev txhawb nqa kev tshawb fawb bioinformatics ntawm sequencing cov ntaub ntawv.
Keeb kwm
Brassica noj(rapeseed) yog ib qho tseem ceeb roj noob qoob loo thiab tus qauv zoo heev rau kev soj ntsuam cov txheej txheem ntawm polyploid speciation, evolution thiab xaiv.Txawm li cas los xij, txawm tias cov tsiaj qus los yog cov neeg pub nyiaj hauv tsev yog niam txiv progenitors thiab cov noob caj noob ces uas tau pab txhawb kev yug me nyuam thiab kev txhim kho hauv rapeseed tseem tsis tau paub.
Cov ntaub ntawv thiab cov txheej txheem
Khoom siv:588 uaB. nojKev nkag mus tau koom nrog hauv txoj kev tshawb no, suav nrog 466 los ntawm Asia, 102 los ntawm Tebchaws Europe, 13 los ntawm North America, thiab 7 los ntawm Australia.Raws li cov ntaub ntawv tus cwj pwm loj hlob, cov ntaub ntawv no tau muab faib ua peb hom ecotypes;caij nplooj ntoos hlav (86 accessions), lub caij ntuj no (74 accessions), thiab semi-lub caij ntuj no (428 accessions).
Sequence:Nruab nrab kwv yees.5 × (xws li 3.37 × 7.71 ×)
Sequence platform:Illumina Hiseq 4000 Nws
Kev tsim cov ntaub ntawv:4.03 Tb huv data
SNP hu rau:BWA + GATK.5,294,158 SNPs thiab 1,307,151 InDels tau txais.
Cov txiaj ntsig
Keeb kwm ntawm B. napus
B. nojIb tug subgenome hloov zuj zus los ntawm cov poj koob yawm txwv ntawm European turnip.Ib qho kev tshwm sim gene ntws los ntawm European turnip mus rauB. npos Ib subgenome tshwm sim ~ 106–1170 xyoo dhau los.B. nojC subgenome yuav tau hloov zuj zus los ntawm cov poj koob yawm txwv ntawm cov kab no.yawm txiv ntawmB. nojcais los ntawm cov poj koob yawm txwv ntawm plaub B. oleracea subspecies, nrog cov noob tam sim no ntws mus rau hauvB. noj~ 108-898 xyoo dhau los.B. nojC subgenome muaj keeb kwm nyuaj dua li A Subgenome.Ib tug muaj zog bottleneck tsim nyob rau hauv ob qho tib si subgenomes thaum lub sij hawmB. nojevolution.Lub caij ntuj no thiab ib nrab lub caij ntuj noB. nojecotypes diverged ~ 60 xyoo dhau los, whereas lub caij ntuj no thiab caij nplooj ntoos hlavB. nojdiverged ~ 416 xyoo dhau los, thiab oilseed thiab non-oilseedB. nojdiverged ~ 277 xyoo dhau los.
Fig. 2 Pej xeem qauv ntawm 588 B. napus accessions thiab 199 ntawm B. rapa accessions.
Fig. 3 Pej xeem qauv ntawm 588 B. napus accessions thiab 119 ntawm B. oleracea accessions
Kev xaiv cov cim thiab cov kev tshawb fawb ntawm Genome-wide.
Thaum lub sij hawm thawj theem ntawm kev txhim kho (FSI), ntau cov noob caj noob ces poob hauv B. napus C subgenome dua li hauv A subgenome.Tsawg dua kev sib txawv ntawm cov noob caj noob ces tshwm sim thaum lub sijhawm FSI dua li lub sijhawm thib ob ntawm kev txhim kho (SSI).Cov noob hauv cheeb tsam SSI-xaiv cov teeb liab tau ua kom muaj kev ntxhov siab, kev loj hlob thiab txoj hauv kev metabolic.60 loci cuam tshuam nrog 10 lub hom phiaj zoo, suav nrog 5 ntsig txog cov noob qoob loo, 3 txog silique ntev, 4 rau roj cov ntsiab lus, thiab 48 rau cov noob zoo tau txheeb xyuas.
Fig. 4 Genome-wide scanning thiab annotations ntawm cov cheeb tsam xaiv thaum lub sij hawm SSI ntawm B. napus
Transcriptome tsom xam
RNAseq cov ntaub ntawv ntawm 11 cov ntaub so ntswg los ntawm high-roj-cov ntsiab lus thiab ob-tsawg cultivar thiab ib tug tsawg-roj-cov ntsiab lus thiab ob-siab cultivar txheeb xyuas cov noob muaj feem xyuam rau cov txheej txheem glucosinolate biosynthetic tau ntau dua- sawv cev.
Fig. 5 Txheej txheem cej luam ntawm flowering-lub sij hawm kev cai raws li kev xaiv ntawm kev txhim kho ecotype ntawm B. napus
Kev sib tham
Txoj kev tshawb no tau muab ib qho txiaj ntsig zoo rau kev nkag siab txog keeb kwm thiab kev txhim kho keeb kwm ntawmB. nojthiab yuav pab txhawb qhov kev txiav txim ntawm cov caj ces hauv paus ntawm qhov tseem ceeb agronomic complex zoo.Qhov tseem ceeb ntawm SNPs cuam tshuam nrog cov txiaj ntsig zoo, cov cim xaiv thiab cov noob neeg sib tw yuav ua rau muaj txiaj ntsig zoo rau yav tom ntej, tshwj xeeb tshaj yog hauv kev txhim kho cov txiaj ntsig, cov noob zoo, cov ntsiab lus roj, thiab kev hloov pauv ntawm cov qoob loo allopolyploid tsis ntev los no thiab nws cov txheeb ze.
Kev siv
Tag nrho-genome resequencing qhia Brassica napus keeb kwm thiab caj ces loci koom nrog hauv nws txoj kev txhim kho [J].Nature CommunicationsXyoo 2019, 10(1).
Xov xwm thiab qhov tseem ceeb Lub hom phiaj ntawm kev sib koom cov xwm txheej ua tiav tshiab nrog Biomarker Technologies, khaws cov kev tshawb fawb tshiab thiab cov txuj ci tseem ceeb siv thaum kawm.
Post lub sij hawm: Jan-05-2022