GENOME EVOLUTION
Kev sib piv genome tsom xam qhov tseem ceeb ntawm transposon-mediated genome expansion thiab evolutionary architecture ntawm 3D genomic folding hauv paj rwb
Nanopor sequencing |Hi-C |PacBio sequencing |Illumina |RNA-sequencing |3D genome architecture |Transposon |Kev sib piv genomics
Nyob rau hauv txoj kev tshawb no, Biomarker Technologies tau muab kev txhawb nqa ntawm Nanopore sequencing, Hi-C thiab cuam tshuam txog bioinformatic tsom xam.
Abstract
Transposable element (TE) amplification tau raug lees paub tias yog lub zog tsav tsheb sib haum xeeb genome loj nthuav dav thiab hloov pauv, tab sis qhov tshwm sim rau shaping 3D genomic architecture tseem tsis paub ntau hauv cov nroj tsuag.Ntawm no, peb qhia txog kev siv-qib genome sib dhos rau peb hom paj rwb xws li peb-fold hauv genome loj, uas yogGossypium rotundifolium yog ib tsob nroj(K2),G. arboreum(A2), thiabG. raimondii(D5), siv Oxford Nanopore Technologies.Comparative genome analyzes sau cov ntsiab lus ntawm cov kab mob tshwj xeeb TE amplification uas ua rau muaj qhov sib txawv loj ntawm genome (K2, 2.44 Gb; A2, 1.62 Gb; D5, 750.19 Mb), thiab qhia txog cov ntsiab lus tseem ceeb ntawm noob caj noob ces thiab kev sib raug zoo ntawm cov genomes.Peb pom tias kwv yees li ntawm 17% ntawm cov noob caj noob ces pom cov xwm txheej chromatin hloov pauv ntawm cov nquag ("A") thiab tsis ua haujlwm ("B"), thiab TE amplification tau cuam tshuam nrog kev nce ntawm qhov feem ntawm A compartment hauv thaj chaw gene (~ 7,000 genes. ) hauv K2 thiab A2 txheeb ze rau D5.Tsuas yog 42% ntawm topologically associating domain (TAD) ciam teb tau khaws cia ntawm peb genomes.Peb cov ntaub ntawv cuam tshuam txog kev nthuav dav ntawm TEs tsis ntev los no tom qab tsim cov kab ke tshwj xeeb TAD ciam teb.Txoj kev tshawb no pom lub luag haujlwm ntawm transposon-mediated genome expansion nyob rau hauv evolution ntawm ntau dua-order chromatin qauv hauv cov nroj tsuag.
Cov txheeb cais tseem ceeb ntawm kev sib dhos genome
Daim duab.Genome los ua ke thiab cov lus piav qhia ntawm G. rotundifolium (K2)
Xov xwm thiab qhov tseem ceeb Lub hom phiaj ntawm kev sib koom cov xwm txheej ua tiav tshiab nrog Biomarker Technologies, khaws cov kev tshawb fawb tshiab thiab cov txuj ci tseem ceeb siv thaum kawm.
Post lub sij hawm: Jan-05-2022