High-throughput genotyping, tshwj xeeb tshaj yog nyob rau hauv loj-scale pej xeem, yog ib tug tseem ceeb kauj ruam nyob rau hauv cov kev tshawb fawb ntawm genetic koom haum, uas muab genetic hauv paus rau functional gene discovery, evolutionary tsom xam, thiab lwm yam ) yog qhia kom txo qis tus nqi sequencing ib tus qauv, thaum tswj kev ua haujlwm tsim nyog ntawm kev tshawb nrhiav caj ces.Qhov no feem ntau ua tiav los ntawm kev rho tawm qhov txwv tsis pub nyob rau hauv qhov loj me, uas yog lub npe txo cov tsev qiv ntawv sawv cev (RRL).Specific-locus amplified fragment sequencing (SLAF-Seq) yog ib lub tswv yim tsim tus kheej rau de novo SNP nrhiav thiab SNP genotyping ntawm cov neeg coob.
Technical workflow
SLAF vs Existing RRL txoj kev
Qhov zoo ntawm SLAF
Kev tshawb nrhiav kev tshawb fawb ntau dua genetic marker efficiency- Ua ke nrog cov thev naus laus zis ntau dhau los ua ke, SLAF-Seq tuaj yeem ua tiav ntau pua txhiab tus cim pom nyob rau hauv tag nrho cov genome kom ua tiav qhov kev thov ntawm ntau hom kev tshawb fawb, nrog lossis nrog peb cov ntaub ntawv siv genome.
Customized & Flexible sim tsim- Rau cov hom phiaj tshawb fawb sib txawv lossis hom, cov tswv yim sib txawv ntawm kev zom zaub mov muaj xws li ib leeg-enzyme, dual-enzyme thiab ntau-enzyme digestion.Lub tswv yim digestion yuav raug soj ntsuam ua ntej hauv silico kom paub tseeb tias kev tsim cov enzyme zoo.
High efficiency nyob rau hauv enzymatic digestion- Kev tsim ua ntej enzymatic digestion muab ntau qhov sib npaug ntawm SLAFs ntawm chromosome.Fragment collection npaum tuaj yeem ua tiav ntau dua 95%.
Tsis txhob rov ua ntu zus- Feem pua ntawm cov lus rov qab ua ntu zus hauv SLAF-Seq cov ntaub ntawv raug txo qis dua 5%, tshwj xeeb tshaj yog nyob rau hauv cov tsiaj uas muaj qib siab ntawm cov khoom rov ua dua, xws li nplej, pob kws, thiab lwm yam.
Tus kheej-tsim bioinformatic workflow- BMK tau tsim ib qho kev sib koom ua ke ntawm bioinformatic workflow siv rau SLAF-Seq thev naus laus zis kom ntseeg tau tias muaj kev ntseeg siab thiab raug ntawm cov khoom siv kawg.
Kev siv SLAF
Genetic linkage map
High-density genetic daim ntawv qhia kev tsim kho thiab kev txheeb xyuas ntawm loci tswj cov paj hom zoo hauv Chrysanthemum (Chrysanthemum x morifolium Ramat.)
Phau ntawv Journal: Horticulture Research Published: 2020.7
GWAS
Kev txheeb xyuas tus neeg sib tw gene cuam tshuam nrog cov ntsiab lus isofavone hauv cov noob taum pauv siv genome-wide koom haum thiab kev sib txuas ua ke
Phau ntawv Journal: The Plant Journal Published: 2020.08
Evolutionary noob caj noob ces
Pej xeem genomic tsom xam thiab de novo los ua ke qhia lub hauv paus chiv keeb ntawm weedy mov raws li ib tug evolutionary kev ua si
Phau ntawv Journal: Molecular Plant Published: 2019.5
Bulked Segregant Analysis (BSA)
GmST1, uas encodes ib sulfotransferase, confers kuj rau soybean mosaic kab mob hom G2 thiab G3
Phau ntawv Journal: Cog, Cell & Ib puag ncig luam tawm: 2021.04
Kev siv
Sun X, Liu D, Zhang X, et al.SLAF-Seq: ib txoj kev ua tau zoo ntawm qhov loj-scale de novo SNP nrhiav thiab genotyping siv high-throughput sequencing [J].Plos ib, 2013, 8(3):e58700
Song X, Xu Y, Gao K, et al.High-density genetic daim ntawv qhia kev tsim kho thiab kev txheeb xyuas ntawm loci tswj cov paj hom zoo hauv Chrysanthemum (Chrysanthemum × morifolium Ramat.).Hortic Res.Xyoo 2020; 7:108.
Wu D, Li D, Zhao X, et al.Kev txheeb xyuas tus neeg sib tw gene cuam tshuam nrog cov ntsiab lus isoflavone hauv cov noob taum pauv siv genome-wide koom haum thiab kev sib txuas ua ke.cog J. 2020;104(4): 950-963.
Sun J, Ma D, Tang L, et al.Population Genomic Analysis thiab De Novo Assembly nthuav tawm lub hauv paus chiv keeb ntawm Weedy Rice raws li Evolutionary Game.Mol cog.2019; 12(5): 632-647.Mol cog.2018;11(11): 1360-1376.
Zhao X, Jing Y, Luo Z, et al.GmST1, uas encodes ib sulfotransferase, confers kuj rau soybean mosaic kab mob hom G2 thiab G3.Plant Cell Environ.2021; 10.1111/pce.14066
Post lub sij hawm: Jan-04-2022