Tshawb nrhiav cov qauv qhia cov noob ntawm qhov chaw qub ntawm lub cev yog qhov tseem ceeb rau kev nkag siab txog hom thiab kev ua haujlwm ntawm nws cov hlwb.Txawm li cas los xij, tam sim no spatial transcriptome txoj kev tsom xam muaj cov kev txwv xws li kev xa tawm tsawg lossis tsis txaus daws teeb meem.BMKMANU S1000 Spatial Chip, tsim los ntawm BMKMANU, tuaj yeem tshawb xyuas cov ntaub ntawv ntawm cov noob caj ces hauv cov ntaub so ntswg ua tiav ntawm subcellular daws teeb meem, ua kom muaj kev txhais lus tsis zoo ntawm cov ntaub so ntswg.
Npaj txhij los ntsib peb cov khoom tshiab tshaj plaws!Ua kev zoo siab rau nws qhov kev tso tawm, peb muab qhov tshwj xeebYuav Plaub Tau Ib Tug Dawbkev txhawb nqa rau lub sijhawm txwv nkaus xwb.
Khoom Cheebtsam
BMKMANU S1000 spatial nti thiab cov khoom siv reagent txuam nrog:
1) Tissue Optimization Kit, muaj cov reagents rau cov ntaub so ntswg optimization (lub sij hawm zoo tshaj plaws rau cov ntaub so ntswg permeabilization).
2) Gene Expression Kit, muaj cov tshuaj reagents rau kev ntes mRNA hauv cov ntaub so ntswg rau kev npaj tsev qiv ntawv tom ntej thiab kev sib txuas.
3) Cov khoom siv pib: suav nrog cov khoom siv hluav taws xob thermostatic thiab cov ntawv sau cov hlau nplaum sib nqus.
Txoj Cai
Lub nti yog ua raws li micropores thiab microbeads, thiab mRNA tuaj yeem ntes hauv qhov chaw los ntawm Oligo ntawm microbeads.Lub Oligo yog tsim los ntawm plaub ntu: Read1, Spatial Barcode, UMI, thiab Poly(dT)VN.Tom qab cov ntaub so ntswg txuas nrog lub nti, mRNA raug tso tawm los ntawm cov ntaub so ntswg los ntawm permeabilization enzyme.Txij li feem ntau mRNA 3' xaus muaj Poly-A Tail, lawv raug ntes los ntawm Oligo nrog Poly(dT)VN.Tom qab RT-PCR thiab cDNA amplification, lub tsev qiv ntawv npaj thiab sib sau ua ke, spatial txoj hauj lwm tracing tiav los ntawm Spatial Barcode.Qhov no tso cai rau kev tshuaj ntsuam gene hauv cov chaw nyob thiab cov ntaub so ntswg heterogeneity.
Khoom zoo
Daim ntawv thov
Nws tuaj yeem siv tau rau feem ntau ntawm kev tshawb fawb txog kev lom neeg thiab kev kho mob, suav nrog qog nqaij hlav, kab mob, kev tiv thaiv kab mob, thiab kev loj hlob biology, pab kom ua tiav cov kev ua tiav tshiab hauv cov haujlwm no nrog kev tsom xam ntawm qhov chaw ntawm subcellular daws teeb meem.
●Tumor thiab Kab Mob:
Spatial heterogeneity thiab microenvironment ntawm cov qog thiab kab mob
Pib thiab kev loj hlob ntawm cov qog thiab kab mob
Kev kho mob ntawm cov qog thiab kab mob
●Developmental Biology
Spatiotemporal atlas ntawm lub cev
Gene regulatory mechanisms thaum lub sij hawm kev loj hlob
● Teb Kev Nyuaj Siab
Biotic kev nyuaj siab teb
Abiotic kev nyuaj siab teb
● Immunology
Kev tiv thaiv kab mob hauv lub cev
Immune mechanisms ntawm qog thiab kab mob
Pathogenesis ntawm cov kab mob autoimmune
● Kev tshuaj xyuas tshuaj tiv thaiv
Mechanisms ntawm tshuaj tiv thaiv
Kev tshawb fawb thiab kev tsim cov tshuaj tshiab
Case thiab Demo Data
BMKMANU S1000 tau ua tiav-tsim pov thawj hauv ntau pua kis ntawm ntau hom ntaub so ntswg.
