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Proteomics
Proteomics suav nrog kev siv thev naus laus zis rau kev txheeb xyuas tag nrho cov proteins uas muaj cov ntsiab lus ntawm cov cell, cov ntaub so ntswg lossis cov kab mob.Proteomics-raws li thev naus laus zis tau siv ntau lub peev xwm rau kev tshawb fawb sib txawv xws li kev tshawb nrhiav ntau yam kev kuaj mob, cov neeg sib tw rau kev tsim tshuaj tiv thaiv, nkag siab txog cov txheej txheem pathogenicity, kev hloov pauv ntawm cov qauv qhia hauv kev teb rau cov teeb liab sib txawv thiab kev txhais cov kev ua haujlwm protein ntau hauv cov kab mob sib txawv.Tam sim no, quantitative proteomics technologies feem ntau muab faib ua TMT, Label Free thiab DIA quantitative strategies.
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Metabolomics
Lub metabolome yog lub davhlau ya nyob twg downstream khoom ntawm lub genome thiab muaj tag nrho cov ntxiv ntawm tag nrho cov uas tsis muaj-molecular-yuag molecules (metabolites) nyob rau hauv ib tug cell, cov ntaub so ntswg, los yog kab mob.Metabolomics aims los ntsuas qhov dav dav ntawm me me molecules nyob rau hauv cov ntsiab lus ntawm physiological stimuli los yog kab mob xeev.Metabolomics methodologies poob rau hauv ob pawg sib txawv: tsis yog lub hom phiaj metabolomics, ib qho kev tshuaj xyuas dav dav ntawm tag nrho cov ntsuas ntsuas hauv ib qho piv txwv suav nrog cov tshuaj tsis paub siv GC-MS / LC-MS, thiab cov phiaj xwm metabolomics, kev ntsuas ntawm cov pab pawg tshwj xeeb ntawm cov tshuaj lom neeg thiab biochemically annotated metabolites.
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Kev ntsuam xyuas Segregant
Bulked Segregant Analysis (BSA) yog cov txheej txheem siv los txheeb xyuas cov phenotype txuam nrog cov cim caj ces.Lub luag haujlwm tseem ceeb ntawm BSA muaj xaiv ob pawg ntawm cov tib neeg uas muaj kev tawm tsam phenotypes, sib sau cov DNA ntawm txhua tus neeg los ua ob feem ntawm DNA, txheeb xyuas qhov sib txawv ntawm ob lub pas dej.Cov txheej txheem no tau siv dav hauv kev txheeb xyuas cov cim caj ces muaj feem cuam tshuam los ntawm cov noob hom phiaj hauv cov nroj tsuag / tsiaj genomes.
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DNA / RNA Sequencing - Nanopore Sequencer
ONT sequencing yog ib qho molecule real-time hluav taws xob teeb liab sequencing tshuab raws li nanopores, lub hauv paus ntsiab lus ntawm txhua lub platform yog tib yam.Ob-stranded DNA / RNA yuav khi rau nanoporous protein nyob rau hauv biofilm thiab unwinding nyob rau hauv cov hlau lead ntawm lub cev muaj zog protein, nyob rau hauv qhov kev txiav txim ntawm voltage txawv ntawm ob sab ntawm lub biofilm, DNA / RNA strands dhau los ntawm nanopore channel protein ntawm ib tug tej yam. tus nqi.Vim tias qhov sib txawv ntawm cov khoom siv tshuaj sib txawv ntawm cov hauv paus sib txawv ntawm DNA / RNA strand, thaum ib lub hauv paus lossis DNA molecule dhau los ntawm nanopore channel, nws yuav ua rau muaj kev hloov pauv ntawm cov hluav taws xob sib txawv.Los ntawm kev txheeb xyuas thiab sib raug rau cov teeb liab no, cov hom hauv paus sib xws tuaj yeem suav tau, thiab kev tshawb nrhiav lub sijhawm tiag tiag ntawm cov kab ke tuaj yeem ua tiav.
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DNA/RNA sequencing - PacBio Sequencer
PacBio sequencing platform yog ib tug ntev-nyeem sequencing platform, uas yog tseem lub npe hu ua ib tug thib peb-Tim sim no Sequencing (TGS) technologies.Cov thev naus laus zis thev naus laus zis, ib leeg-molecule real-time (SMRT), txhawb kev tsim cov ntawv nyeem nrog kaum ntawm kilo-base ntev.Nyob rau hauv lub hauv paus ntawm "Sequencing-by-Synthesis", ib qho kev daws teeb meem nucleotide yog ua tiav los ntawm Zero-mode waveguide (ZMW), qhov twg tsuas yog ntim hauv qab (qhov chaw ntawm molecule synthesis), yog illuminated.Tsis tas li ntawd, SMRT sequencing feem ntau zam kev tsis sib haum xeeb hauv NGS system, uas feem ntau ntawm PCR amplification cov kauj ruam tsis tas yuav tsum tau ua hauv cov txheej txheem tsim tsev qiv ntawv.
Platform: Sequel II, Revio
