Cov khoom

Genome-wide Association Analysis

Genome-wide Association txoj kev tshawb fawb (GWAS) lub hom phiaj ntawm kev txheeb xyuas cov noob caj noob ces (genotype) uas cuam tshuam nrog cov yam ntxwv tshwj xeeb (phenotype).GWAS txoj kev tshawb fawb soj ntsuam cov cim caj ces hla tag nrho cov genome ntawm cov neeg coob coob thiab kwv yees cov koom haum genotype-phenotype los ntawm kev txheeb cais ntawm cov pej xeem.Nws tau siv dav hauv kev tshawb fawb txog tib neeg cov kab mob thiab kev ua haujlwm gene mining ntawm cov yam ntxwv ntawm cov tsiaj lossis cov nroj tsuag.

Tiv tauj peb

Cov ntsiab lus kev pabcuam

Kev tshwm sim Demo

Tshaj tawm tshwj xeeb

1. Kev Pabcuam Zoo

● Cov Txheej Txheem Ntau Lawm: txij li nws tau tsim nyob rau xyoo 2009, BMKGENE tau ua tiav ntau pua hom phiaj xwm hauv cov pej xeem GWAS kev tshawb fawb, pab cov kws tshawb fawb luam tawm ntau tshaj 100 kab lus, thiab qhov cuam tshuam ntau ntxiv mus txog 500.
● Cov kws tshuaj ntsuam xyuas.
● Kev tshawb xyuas luv luv.
● Cov ntaub ntawv raug mining.

2. Kev Pabcuam Specifications

Hom	Pej xeem Scale	Sequencing lub tswv yim thiab qhov tob
SLAF-GWAS	Qauv nab npawb ≥200	Genome loj <400M, nrog ref-genome, WGS pom zoo
		genome loj ≤ 1G, 100K Cim npe thiab 10X
		1G ≤ Genome loj ≤ 2G, 200K Tags thiab 10X
		genome loj> 2G, 300K Cim npe thiab 10X
WGS-GWAS	Qauv nab npawb ≥200	10X rau txhua tus qauv

3. Kev xaiv cov khoom siv

Ntau hom, subspecies, landraces/genebanks/mixed tsev neeg/wild resources

Txawv ntau yam, subspecies, landraces

Ib nrab-sib family/full-sib family/ wild resources

4. Bioinformation Analysis

● Kev txheeb xyuas kev sib koom ua ke thoob plaws genome
● Kev tshuaj xyuas thiab tshuaj xyuas ntawm SNP tseem ceeb
● Cov lus piav qhia ua haujlwm ntawm tus neeg sib tw gene

5. Kev Pabcuam Ua Haujlwm Zoo

Kev sim tsim

Qauv xa tuaj

RNA rho tawm

Kev tsim kho tsev qiv ntawv

Sequencing

Cov ntaub ntawv tsom xam

Kev pab cuam tom qab muag

Yav dhau los: Ib leeg- nucleus RNA Sequencing

Tom ntej:

ib.Phenotype QC

Zaus faib histogram

Phenotype txheeb cais

b.Kev txheeb xyuas koom haum (Model: GEMMA, FaST-LMM, EMMAX)

QQ Ploj

Manhattan Ploj

Xyoo	Phau ntawv	IF	Title
2022	NC	17.69 Nws	Genomic hauv paus ntawm giga-chromosomes thiab giga-genome ntawm tsob ntoo peony Paeonia ostii
2015	NP	7.43 ib	Domestication footprints thauj cov cheeb tsam genomic ntawm agronomic tseem ceeb hauv cov taum pauv
2018	MP	9.32 ib	Tag nrho-genome resequencing ntawm ib tug thoob ntiaj teb sau ntawm rapeseed accessions qhia genetic hauv paus ntawm lawv ecotype divergence
2022	HR	7.29 ib	Kev tshuaj ntsuam genome-wide koom haum muab cov kev nkag siab molecular rau hauv kev hloov pauv ntawm cov noob txiv ntoo loj