Cov khoom

Cov kab mob fungal

Biomarker Technologies muab kev tshawb fawb genome, zoo genome thiab pene-ua tiav genome ntawm fungal nyob ntawm lub hom phiaj tshawb fawb tshwj xeeb.genome sequencing, sib dhos thiab kev ua haujlwm annotation tuaj yeem ua tiav los ntawm kev sib txuas ua ke Next-tiam sequencing + Peb tiam sequencing kom ua tiav qib siab genome los ua ke.Hi-C thev naus laus zis kuj tseem tuaj yeem ua haujlwm los pab txhawb genome los ua ke ntawm qib chromosome.

Platform:PacBio Sequel II

Nanopor PromethION P48

Illumina NovaSeq Platform

Tiv tauj peb

Cov ntsiab lus kev pabcuam

Cov txiaj ntsig Demo

Kev Pabcuam Zoo

● Ntau lub tswv yim sib txuas ua ke muaj rau cov hom phiaj tshawb fawb sib txawv

● Muaj kev paub dhau los hauv fungi genome los ua ke nrog ntau dua 10,000 pene-tiav genome assembled.

● Pab pawg kws tshaj lij tom qab muag kev pab txhawb nqa ua tiav cov kev tshawb fawb tshwj xeeb ntxiv.

Service Specifications

Kev pabcuam	Sequence Strategy	Zoo guaranteed	Kwv yees lub sijhawm tig rov qab
Fungal daim ntawv qhia zoo	Illumina 50X + Nanopore 100X	Contig N50≥2 Mb	35 hnub ua haujlwm
Fungal daim ntawv qhia zoo	PacBio HiFi 30X	Contig N50≥2 Mb	35 hnub ua haujlwm
Fungal pene-ua tiav daim ntawv qhia	Illumina 50X + Nanopore 100X (Pacbio HiFi 30X) + Hi-C 100X	Chromosome anchoring ratio> 90%	45 hnub ua haujlwm

Bioinformatics tsom xam

● Raw cov ntaub ntawv zoo tswj

● Genome los ua ke

● Kev tshuaj ntsuam xyuas genome

● Gene muaj nuj nqi annotation

● Sib piv genomic tsom xam

Sample Requirements thiab Delivery

Cov Qauv Xav Tau:

RauDNA rho tawm:

Hom qauv	Tus nqi	Nco ntsoov	Purity
DNA rho tawm	1.2g 1	20 ng / μl	OD 260/280 = 1.6-2.5

Rau cov ntaub so ntswg:

Hom qauv

Pom zoo coj mus kuaj

Sample cia thiab xa khoom

Unicellular fungus

Saib cov poov xab nyob rau hauv lub microscope thiab sau lawv nyob rau hauv lawv exponential theem

Hloov cov kab lis kev cai (muaj kwv yees li 3-4.5e9 hlwb) rau hauv 1.5 lossis 2 ml eppendorf.(Ceev rau dej khov)

Centrifuge lub raj rau 1 min ntawm 14000 g kom khaws cov kab mob thiab tshem tawm cov supernatant kom zoo

Kaw lub raj thiab khov cov kab mob hauv cov kua nitrogen rau tsawg kawg 1-3 teev.Khaws lub raj rau hauv -80 ℃ fridge.

Kho cov qauv hauv cov kua nitrogen rau 3-4 teev thiab khaws cia hauv cov kua nitrogen los yog -80 degree mus rau lub sijhawm ntev.Qauv shipping nrog qhuav-dej khov yog yuav tsum tau.

Macro fungus

Cov ntaub so ntswg nyob rau theem nquag loj hlob yog pom zoo.

Yaug cov ntaub so ntswg nrog dej tsis muaj endotoxin, tom qab ntawd 70% ethonal.

Txuag cov qauv hauv cov raj cryo.