Illumina thiab BGI
Nta
●Platforms:Illumina NovaSeq 6000, NovaSeq, HiSeq X Ten thiab BGI-DNB-T7
●Sequence hom:PE 50, PE 100, PE 150, PE 250
●Kev tswj xyuas cov tsev qiv ntawv zoo ua ntej ua ntu zus
●Sequencing cov ntaub ntawv xa thiab QC:xa ntawm QC daim ntawv qhia thiab cov ntaub ntawv nyoos hauv fastq hom tom qab demultiplexing thiab lim Q30 nyeem.
Kev Pabcuam Zoo
●Versatility ntawm Sequence Services:tus neeg siv khoom tuaj yeem xaiv ua raws li txoj kab, ntws ntawm tes lossis cov ntaub ntawv ntau npaum li cas.
●Versatility ntawm platforms:DNB cov tsev qiv ntawv tuaj yeem hloov mus rau Illumina platforms
●Kev paub dav dav ntawm Illumina sequencing platform:nrog ntau txhiab txoj haujlwm kaw nrog ntau hom.
●Kev xa khoom ntawm QC daim ntawv qhia:nrog rau kev ntsuas zoo, cov ntaub ntawv raug thiab tag nrho cov kev ua tau zoo ntawm qhov kev sib txuas ua ke.
●Mature sequencing txheej txheem:nrog lub sijhawm luv luv.
●Rigorous Quality Control: Peb ua raws li QC nruj me ntsis kom lav qhov kev xa khoom ntawm cov txiaj ntsig zoo.
Cov Qauv Xav Tau *
Partial Lane Sequencing
| Tus nqi ntawm cov ntaub ntawv (X) | Concentration (qPCR/nM) | Ntim |
| X ≤ 50 Gb | ≥ 2 nM | ≥ 20 μl |
| 50 Gb ≤ X < 100 Gb | ≥ 3 nM | ≥ 20 μl |
| X ≥ 100 Gb | ≥ 4 nM | ≥ 20 μl |
Ib Txoj Kev (Illumina)
| Platform | Concentration (qPCR/nM) | Ntim |
| HiSeq X Ten | ≥ 2 nM | ≥ 20 μl |
| NovaSeq 6000 SP | ≥ 1 nM | ≥ 25 μl |
| NovaSeq 6000 S4 | ≥1.5nM | ≥ 25 μl |
| NovaSeq X | ≥1.5nM | ≥ 25 μl |
| BGI-DNBSEQ-T7 | ≥1.5nM | ≥ 25 μl |
Ntxiv nrog rau qhov concentration thiab tag nrho cov nyiaj, tus qauv tsim nyog ncov kuj yuav tsum tau.
Thov hu rau peb yog tias koj cov qauv ua tsis tau raws li cov khoom siv pib.
Kev Pabcuam Ua Haujlwm
Tswj tsev qiv ntawv zoo
Sequencing
Kev tswj cov ntaub ntawv zoo
Qhov project xa
QC daim ntawv qhia
Ib daim ntawv qhia txog qhov zoo ntawm lub tsev qiv ntawv yog muab ua ntej sequencing, ntsuam xyuas cov tsev qiv ntawv, thiab fragmentation.
Sequencing QC daim ntawv qhia
Table 1. Kev txheeb cais ntawm cov ntaub ntawv sib txuas.
| Qauv ID | BMKID | Raw nyeem | Cov ntaub ntawv raw (bp) | Kev nyeem ntawv huv (%) | Q20 (%) | Q30 (%) | GC(%) |
| C_01 | BMK_01 | 22, 870, 120 | 6,861,036,000 ib | 96.48 ib | 99.14 ib | 94.85 ib | 36.67 ib |
| C_02 | BMK_02 | 14, 717, 867 ib | 4, 415, 360, 100 | 96.00 Nws | 98.95 ib | 93.89 ib | 37.08 Nws |
Daim duab 1. Kev faib khoom zoo raws li nyeem hauv txhua tus qauv
Daim duab 2. Kev faib tawm hauv paus
Daim duab 3. Kev faib cov ntawv nyeem hauv cov ntaub ntawv sib txuas
