Cov khoom

BMKMANU S1000 Spatial Transcriptome

Lub koom haum spatial ntawm cov hlwb ua lub luag haujlwm tseem ceeb hauv ntau yam txheej txheem lom neeg, xws li kev tiv thaiv kab mob, kev loj hlob ntawm embryo, thiab lwm yam. Spatial transcriptome sequencing, uas qhia txog noob caj noob ces profiling thaum khaws cov ntaub ntawv ntawm spatial txoj hauj lwm, tau muab kev pom zoo rau hauv cov ntaub so ntswg transcriptome.Nrog rau kev txhim kho thev naus laus zis, cov ntaub so ntswg ntshiab ultra-clear morphology thiab qhov sib txawv ntawm cov qauv ntawm spatial molecular qhia yuav tsum tau kawm nrog kev daws teeb meem siab dua.BMKGENE muab kev qhia dav dav, ib-nres spatial transcriptome sequencing kev pabcuam los ntawm cov qauv mus rau kev nkag siab txog kev lom neeg.

Spatial transcriptomics technologies txhawb cov kev xav tshiab hauv kev tshawb fawb sib txawv los ntawm kev daws cov noob qhia profile nrog cov ntsiab lus spatial hauv cov qauv heterogenous.

Spatial transcriptome nti: BMKMANU S1000

Platform: Illumina NovaSeq Platform

Tiv tauj peb

Cov ntsiab lus kev pabcuam

Bioinformatics

Cov txiaj ntsig Demo

BMKMANU S1000 Spatial Transcriptome Technical Scheme

Qhov zoo ntawm BMKMANU S1000

1) Sub-cellular daws teeb meem: Txhua qhov chaw ntes muaj> 2 lab spatial Barcoded Spots nrog txoj kab uas hla ntawm 2.5 µm thiab qhov sib nrug ntawm 5 µm ntawm cov chaw nruab nrab, ua kom muaj kev soj ntsuam spatial transcriptome nrog sub-cellular daws teeb meem (5 µm).

2) Kev tshuaj xyuas ntau qib kev daws teeb meem: Hloov pauv ntau theem kev tshuaj ntsuam txij li 100 μm mus rau 5 μm los daws cov ntaub so ntswg sib txawv ntawm qhov kev daws teeb meem zoo.

3) Cov ntaub ntawv qhia ntxaws ntxaws: Cov ntawv sau tau raug ntes los ntawm tag nrho cov ntaub so ntswg tuaj yeem txheeb xyuas, yam tsis muaj kev txwv ntawm tus naj npawb ntawm cov hom phiaj thiab lub hom phiaj.

Service Specifications

Lub tsev qiv ntawv	Sequencing tswv yim	Cov ntaub ntawv tso zis pom zoo
S1000 cDNA qiv	BMKMANU S1000-Illumina PE 150	60 Gb / qauv

Qauv Yuav Tsum Tau

Qauv	Tus lej	Loj	RNA Zoo
OCT cov ntaub so ntswg thaiv	2-3 blocks / qauv	Kwv yees li.6.8 x 6.8 x 6.8 mm³	RIN ≥ 7

Yog xav paub ntxiv txog cov qauv qhia kev npaj thiab kev pabcuam kev ua haujlwm, thov koj tham nrog aBMKGENE tus kws tshaj lij

Kev Pabcuam Ua Haujlwm Flow

Kev sim tsim

Qauv xa tuaj

Kev sim ua ntej

Spatial barcoding

Kev tsim kho tsev qiv ntawv

Sequencing

Cov ntaub ntawv tsom xam

Kev pab cuam tom qab muag

Yav dhau los: 10x Genomics Visium Spatial Transcriptome

Tom ntej: Nroj / Tsiaj Tag Nrho Genome Sequencing

Cov ntaub ntawv tsim los ntawm BMKMANU S1000 yog tshuaj xyuas siv software "BSTMATRIx", uas yog tus kheej tsim los ntawm BMKGENE, suav nrog:

1) Gene qhia matrix tiam
2) HE ua cov duab
3) Tau tshaj downstream thib peb-tog software rau kev tsom xam
4) Online "BSTViewer" pab kom tau txais cov txiaj ntsig pom ntawm cov kev daws teeb meem sib txawv.

1.Spot clustering

s1000 (4)