● Ntau lub tswv yim sib txuas ua ke muaj rau cov hom phiaj tshawb fawb sib txawv
● Cov kab mob ua tiav genome nrog 0 Gap lav.
● Muaj kev paub dhau los ntawm cov kab mob genome los ua ke nrog ntau dua 10,000 ua tiav genome sib sau ua ke.
● Pab pawg kws tshaj lij tom qab muag kev pab txhawb nqa ua tiav cov kev tshawb fawb tshwj xeeb ntxiv.
SequencingTswv yim | Lub tsev qiv ntawv | Zoo guaranteed | Kwv yees lub sijhawm tig rov qab |
Nanopor 100X + Illumina 50X | Nanopor 20K PEB 150 | 0 Gap | 30 Hnub |
PacBio HiFi 30X | PacBio 10 K |
● Raw cov ntaub ntawv zoo tswj
● Genome los ua ke
● Kev tshuaj ntsuam xyuas genome
● Gene muaj nuj nqi annotation
● Sib piv genomic tsom xam
RauDNA rho tawm:
Hom qauv | Tus nqi | Nco ntsoov | Purity |
DNA rho tawm | o2m g | 20 ng / μl | OD 260/280 = 1.6-2.5 |
Rau cov ntaub so ntswg:
Hom qauv | Pom zoo coj mus kuaj | Sample cia thiab xa khoom |
Kab mob | Saib xyuas cov kab mob nyob rau hauv lub tshuab kuaj kab mob thiab sau cov kab mob hauv lawv cov theem exponential Hloov cov kab mob kab mob (muaj kwv yees li 3-4.5e9 hlwb) rau hauv 1.5 lossis 2 ml eppendorf.(Ceev rau dej khov) Centrifuge lub raj rau 1 min ntawm 14000 g kom khaws cov kab mob thiab tshem tawm cov supernatant kom zoo Kaw lub raj thiab khov cov kab mob hauv cov kua nitrogen rau tsawg kawg 30 min.Khaws lub raj rau hauv -80 ℃ fridge. | Kho cov qauv hauv cov kua nitrogen rau 3-4 teev thiab khaws cia hauv cov kua nitrogen los yog -80 degree mus rau lub sijhawm ntev.Qauv shipping nrog qhuav-dej khov yog yuav tsum tau. |
1.Circos ntawm cov kab mob genome
2.Coding noob kwv yees
3.Comparative genomics tsom xam: phylogenetic tsob ntoo