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Cov khoom

Kev ntsuam xyuas rau Transposase-Accessible Chromatin nrog High Throughput Sequencing (ATAC-seq)

Kev ntsuam xyuas rau Transposase-Accessible Chromatin nrog High Throughput Sequencing (ATAC-seq) Featured Duab
  • Kev ntsuam xyuas rau Transposase-Accessible Chromatin nrog High Throughput Sequencing (ATAC-seq)
  • Kev ntsuam xyuas rau Transposase-Accessible Chromatin nrog High Throughput Sequencing (ATAC-seq)
  • Kev ntsuam xyuas rau Transposase-Accessible Chromatin nrog High Throughput Sequencing (ATAC-seq)
  • Kev ntsuam xyuas rau Transposase-Accessible Chromatin nrog High Throughput Sequencing (ATAC-seq)
  • Kev ntsuam xyuas rau Transposase-Accessible Chromatin nrog High Throughput Sequencing (ATAC-seq)

ATAC-seq yog ib txoj hauv kev zoo tshaj plaws rau kev soj ntsuam ntawm genome-wide chromatin accessibility, uas yog ib qho tseem ceeb rau ntiaj teb no epigenetic tswj ntawm noob qhia.Sequencing adapters yog muab tso rau hauv qhib chromatin cheeb tsam los ntawm hyperactive Tn5 transposase.Tom qab PCR amplification, lub tsev qiv ntawv sib txuas tau tsim.Tag nrho cov cheeb tsam qhib chromatin tuaj yeem tau txais nyob rau hauv ib qho chaw tshwj xeeb-lub sijhawm, tsis yog tsuas yog txwv rau qhov chaw sib txuas ntawm qhov kev hloov pauv, lossis qee thaj tsam histone hloov kho.


Cov ntsiab lus kev pabcuam

Bioinformatics

Cov txiaj ntsig Demo

Kev Pabcuam Zoo

● Kev Tshawb Fawb Txog Kev Tshawb Fawb: Kev soj ntsuam cov raj xa dej kom tau raws li ntau yam kev xav tau kev tshuaj xyuas;

● Kev Paub Ntau Yam: Ntau pua txoj haujlwm ua tiav kom deb li deb, suav nrog cov qauv tsiaj thiab cov nroj tsuag.

Service Specifications

Platform: Illumina NovaSeq platform

Lub tsev qiv ntawv hom: ATAC-seq

Pom zoo cov ntaub ntawv tso zis: ≥20M nyeem

Cov Qauv Xav Tau

Hom qauv: Cov ntaub so ntswg, cov hlwb nyob, lossis cov hlwb khov thiab lwm yam

Cell xov tooj: ≥ 5 × 105hlwb, tus lej xov tooj raug yog tus yuam sij rau kev ua tiav ntawm kev sim;

Cov ntaub so ntswg hnyav: ≥ 200mg cov ntaub so ntswg tshiab;

Ntshav: ≥ 2 mL

Kev Pabcuam Ua Haujlwm Flow

Qauv QC

Kev sim tsim

coj mus kuaj

Qauv xa tuaj

Pilot kev sim

DNA rho tawm

Kev npaj tsev qiv ntawv

Kev tsim kho tsev qiv ntawv

Sequencing

Sequencing

Cov ntaub ntawv tsom xam

Cov ntaub ntawv tsom xam

Kev pab cuam tom qab muag

Kev pab cuam tom qab muag

  • Yav dhau los:
  • Tom ntej:

    • 表观-02

    1.Heatmap ntawm ATAC nyeem kev faib tawm ntawm TSS thiab cov cheeb tsam uas nyob ib sab (± 3 kb)

    3 (1)2.Kev faib tawm ntawm thaj chaw qhib chromatin hauv cov khoom sib txawv ntawm cov genome

    4 (1)

    3.Differential ncov hu ntawm pab pawg

    5 (1)

    tau ib nqe lus

    Sau koj cov lus ntawm no thiab xa tuaj rau peb

    Khoompawg

    Xa koj cov lus rau peb:

    Ntaus nkag mus tshawb lossis ESC kom kaw