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Soil Metagenomics
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High Quality Ccs Sequencing Provider -
Eukaryotic mRNA sequencing-Illumina – Biomarker Detail:
Advantages
Sample Requirements and Delivery
Library |
Sequencing strategy |
Data recommended |
Quality Control |
Poly A enriched |
Illumina PE150 |
6 Gb
|
Q30≥85% |
Sample Requirements:
Nucleotides:
Purity |
Integrity |
Amount |
OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Limited or no protein or DNA contamination shown on gel. |
For plants: RIN≥6.5;For animals: RIN≥7;28S/18S≥1.0;limited or no baseline elevation |
Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×106 - 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.
Blood samples: Volume≥1 ml
Microorganism: Mass ≥ 1 g
Recommended Sample Delivery
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment:
- Dry-ice: Samples need to be packed in bags and buried in dry-ice.
- RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow
Experiment design
Sample delivery
RNA extraction
Library construction
Sequencing
Data analysis
After-sale services
Product detail pictures:
Related Product Guide:
Trustworthy good quality and excellent credit score standing are our principles, which will help us at a top-ranking position. Adhering towards the tenet of "quality first, buyer supreme" for
High Quality Ccs Sequencing Provider -
Eukaryotic mRNA sequencing-Illumina – Biomarker , The product will supply to all over the world, such as: The Swiss
, Brasilia
, Surabaya
, We strongly believe that technology and service is our base today and quality will create our reliable walls of future. Only we've got better and better quality , could we achieve our customers and ourselves, too. Welcome customers all over the word to contact us for getting further business and reliable relationships. We have been always here working for your demands whenever you require.