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High Performance Genome Annotation - Full-length mRNA sequencing-Nanopore – Biomarker

Service Details

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We generally believe that one's character decides products' top quality, the details decides products' high-quality ,along with the REALISTIC,EFFICIENT AND INNOVATIVE team spirit for Ngs Bioinformatics , Next Generation Sequencing Service , Microsatellite , Together with our efforts, our products and solutions have won the trust of clients and been very salable each here and abroad.

High Performance Genome Annotation - Full-length mRNA sequencing-Nanopore – Biomarker Detail:

Service Advantages

Service Specifications

Bioinformatics analyses

ü Raw data processing

ü Transcript identification

ü Alternative splicing

ü Expression quantification in gene level and isoform level

ü Differential expression analysis

ü Function annotation and enrichment (DEGs and DETs)

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Sample Requirements and Delivery

Sample Requirements:

Nucleotides:

Purity	Integrity	Amount
OD260/280≥1.8；OD260/230≥1.0；Clear peak at 260 nmLimited or no protein or DNA contamination shown on gel.	For plants: RIN≥7.5;For animals: RIN≥8;28S/18S≥1.0;limited or no baseline elevation	Conc. ≥40 ng/μl;Total ≥ 1 μg

Tissue: Weight(dry): ≥1 g

*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.

Cell suspension: Cell count = 3×10⁶ - 1×10⁷

*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×10⁵, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.

Blood samples: Volume≥1 ml

Recommended Sample Delivery

Container: 2 ml centrifuge tube (Tin foil is not recommended)

Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …

Shipment: 1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.

RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

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Sample delivery

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Library construction

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Sequencing

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Data analysis

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After-sale services

Service Work Flow

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Experiment design

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Sample delivery

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RNA extraction

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Library construction

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Sequencing

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Data analysis

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After-sale services

Product detail pictures:

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We can constantly satisfy our respected customers with our good high quality, good price tag and good support due to we have been additional specialist and extra hard-working and do it in cost-effective way for High Performance Genome Annotation - Full-length mRNA sequencing-Nanopore – Biomarker , The product will supply to all over the world, such as: Riyadh , Lisbon , Johor , To achieve reciprocal advantages, our company is widely boosting our tactics of globalization in terms of communication with overseas customers, fast delivery, the best quality and long-term cooperation. Our company upholds the spirit of "innovation, harmony, team work and sharing, trails, pragmatic progress". Give us a chance and we will prove our capability. With your kind help, we believe that we can create a bright future with you together.

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This is a honest and trustworthy company, technology and equipment are very advanced and the prodduct is very adequate, there is no worry in the suppliment.

By Marcia from Vancouver - 2017.02.18 15:54

This is the first business after our company establish, products and services are very satisfying, we have a good start, we hope to cooperate continuous in the future!

By Brook from Plymouth - 2018.06.05 13:10