ʻO nā RNA liʻiliʻi he ʻano pōkole non-coding RNA me ka lōʻihi o 18-30 nt, me ka miRNA, siRNA a me ka piRNA.Ua hōʻike nui ʻia kēia mau RNA liʻiliʻi e pili ana i nā kaʻina hana ola like ʻole e like me ka hoʻohaʻahaʻa ʻana o ka mRNA, ka pāpā ʻana i ka unuhi, ka hoʻokumu ʻana o heterochromatin, a me nā mea ʻē aʻe. ʻO ka papa hana hoʻopaʻa ʻana kaʻina hana maʻamau a me ka ʻimi ʻikepili kiʻekiʻe.Ma ke kumu o ka ʻikepili RNA-seq, hiki i ka loiloi maʻamau ke hoʻokō i ka ʻike miRNA a me ka wānana, ka wānana gene target miRNA, annotation a me ka nānā ʻana i ka ʻōlelo.Hiki i ka loiloi holomua ka huli ʻana a me ka unuhi ʻana o ka miRNA, ka hana kiʻi Venn, ka miRNA a me ka kūkulu ʻana i ka ʻoihana gene.