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ʻO ke kaʻina hana DNA/RNA -PacBio Sequencer
ʻO PacBio sequencing platform kahi paena heluhelu lōʻihi, ʻike ʻia ʻo ia kekahi o nā ʻenehana Sequencing Third-Generation Sequencing (TGS).ʻO ka ʻenehana koʻikoʻi, hoʻokahi-molele manawa maoli (SMRT), hoʻoikaika i ka hana o nā heluhelu me nā ʻumi kilo-base ka lōʻihi.Ma ke kumu o "Sequencing-by-Synthesis", ua hoʻokō ʻia ka hoʻonā nucleotide hoʻokahi e Zero-mode waveguide (ZMW), kahi i hoʻomālamalama ʻia ai ka leo liʻiliʻi ma lalo (kahi o ka mole synthesis).Eia hou, pale ka SMRT sequencing nui i ka sequence-specific bias in NGS system, no ka mea, ʻaʻole koi ʻia ka hapa nui o nā ʻanuʻu PCR amplification ma ke kaʻina hana hale waihona.
