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Hoʻokahi-nucleus RNA Sequencing
ʻO ka holomua o ka hopu ʻana i ke kelepona hoʻokahi a me ka ʻenehana kūkulu hale waihona puke me ka hui pū ʻana me ke kaʻina hana kiʻekiʻe e hiki ai i nā haʻawina hōʻike gene ma ke kumu cell-by-cell.Hāʻawi ia i kahi ʻōnaehana hohonu a piha hoʻi i nā heluna cell paʻakikī, kahi e pale nui ai i ka huna ʻana i ko lākou heterogeneity ma ka lawe ʻana i ka awelika o nā cell āpau.
Eia nō naʻe, ʻaʻole kūpono kekahi mau cell e hana ʻia i hoʻokuʻu ʻia hoʻokahi-cell, no laila, pono nā ʻano hana hoʻomākaukau ʻē aʻe - ka unuhi ʻana o ka nucleus mai nā ʻiʻo, ʻo ia hoʻi, lawe pololei ʻia ka nucleus mai nā ʻiʻo a i ʻole cell a hoʻomākaukau ʻia i hoʻokahi-nucleus suspension no hoʻokahi- ke kaʻina ʻana o ke kelepona.
Hāʻawi ʻo BMK i ka 10× Genomics ChromiumTM i hoʻokumu ʻia i ka lawelawe hoʻokaʻina RNA pū hoʻokahi.Ua hoʻohana nui ʻia kēia lawelawe i nā haʻawina e pili ana i nā haʻawina e pili ana i nā maʻi, e like me ka hoʻokaʻawale ʻana o ka cell immune, heterogeneity tumor, tissue development, etc.
Kīpī transcriptome āpau: 10× Genomics
Papahana: Illumina NovaSeq Platform
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BMKMANU S1000 Spatial Transcriptome
Kū ka Spatial transcriptomics ma ke alo o ka ʻepekema ʻepekema, e hāʻawi ana i ka poʻe noiʻi e ʻimi i nā hiʻohiʻona hōʻike gene paʻakikī i loko o nā ʻiʻo me ka mālama ʻana i kā lākou pōʻaiapuni spatial.Ma waena o nā paepae like ʻole, ua hoʻomohala ʻo BMKGene i ka BMKManu S1000 Spatial Transcriptome Chip, e kaena ana i kahihoʻonui i ka hoʻonāo 5µM, a hiki i ka laulā subcellular, a hikinā hoʻonohonoho hoʻonā pae nui.ʻO ka puʻupuʻu S1000, e hōʻike ana ma kahi o 2 miliona mau kiko, hoʻohana i nā microwells i hoʻopaʻa ʻia me nā pahu i hoʻopiha ʻia me nā probes hopu hopu.Ua hoʻomākaukau ʻia kahi waihona cDNA, i hoʻonui ʻia me nā barcodes spatial, mai ka chip S1000 a ma hope i hoʻonohonoho ʻia ma ke kahua ʻo Illumina NovaSeq.ʻO ka hui pū ʻana o nā hōʻailona spatially barcoded a me nā UMI e hōʻoia i ka pololei a me ka kikoʻī o ka ʻikepili i hana ʻia.Aia ka hiʻohiʻona kūʻokoʻa o ka chip BMKManu S1000 i kona versatility, e hāʻawi ana i nā hoʻonohonoho hoʻonā nui-level i hiki ke hoʻopili maikaʻi ʻia i nā ʻāpana like ʻole a me nā pae kikoʻī.Hoʻonohonoho kēia adaptability i ka chip ma ke ʻano he koho maikaʻi loa no nā haʻawina transcriptomics spatial like ʻole, e hōʻoia ana i ka clustering spatial pololei me ka leo liʻiliʻi.
Ke hoʻohana nei i ka chip BMKManu S1000 a me nā ʻenehana transcriptomic spatial ʻē aʻe, hiki i nā mea noiʻi ke loaʻa ka ʻike maikaʻi aʻe o ka hoʻonohonoho spatial o nā cell a me nā pilina molekala paʻakikī e kū nei i loko o nā ʻiʻo, e hāʻawi ana i nā ʻike waiwai nui i nā mīkini i lalo o nā kaʻina hana ola i loko o nā ʻano ākea. oncology, neuroscience, developmental biology, immunology and botanical studies.
Papahana: BMKManu S1000 chip a me Illumina NovaSeq
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10x Genomics Visium Spatial Transcriptome
ʻO Spatial transcriptomics kahi ʻenehana ʻokiʻoki e hiki ai i nā mea noiʻi ke noiʻi i nā hiʻohiʻona hōʻike gene i loko o nā ʻiʻo i ka wā e mālama ana i kā lākou pōʻaiapili spatial.ʻO kahi kahua ikaika ma kēia kikowaena ʻo 10x Genomics Visium i hui pū ʻia me ke kaʻina Illumina.Aia ke kumumanaʻo o 10X Visium ma kahi puʻupuʻu kūikawā me kahi wahi hopu i koho ʻia kahi i waiho ʻia ai nā ʻāpana kiko.Aia kēia wahi hopu i nā wahi barcoded, e pili ana kēlā me kēia me kahi wahi kikoʻī i loko o ka ʻiʻo.Hoʻopaʻa inoa ʻia nā molekala RNA i hopu ʻia mai ka ʻiʻo me nā molecular identifiers (UMIs) i ka wā o ke kaʻina hana unuhi.Hāʻawi kēia mau wahi barcoded a me nā UMI i ka palapala ʻāina kikoʻī pololei a me ka helu ʻana o ka hōʻike gene ma kahi hoʻonā hoʻokahi.ʻO ka hui pū ʻana o nā hōʻailona spatially barcoded a me nā UMI e hōʻoia i ka pololei a me ka kikoʻī o ka ʻikepili i hana ʻia.Ma ka hoʻohana ʻana i kēia ʻenehana Spatial Transcriptomics, hiki i nā mea noiʻi ke loaʻa ka ʻike hohonu o ka hoʻonohonoho spatial o nā cell a me nā pilina molekala paʻakikī e kū nei i loko o nā ʻiʻo, e hāʻawi ana i nā ʻike koʻikoʻi i nā hana i lalo o nā kaʻina biological i nā ʻano he nui, me ka oncology, neuroscience, developmental biology, immunology. , a me nā haʻawina botanical.
Papahana: 10X Genomics Visium a me Illumina NovaSeq
