-
Illumina a me BGI
ʻO ka ʻenehana Sequencing Illumina, i hoʻokumu ʻia ma Sequencing by Synthesis (SBS), he hana hou NGS i kākoʻo ʻia i ka honua, kuleana no ka hoʻomohala ʻana ma luna o 90% o ka ʻikepili sequencing o ka honua.ʻO ke kumumanaʻo o SBS e pili ana i ke kiʻi ʻana i ka fluorescently labeled reversible terminator i ka hoʻohui ʻia ʻana o kēlā me kēia dNTP, a ma hope i hoʻokaʻawale ʻia e ʻae i ka hoʻohui ʻana i ka waihona aʻe.Me nā dNTP i hoʻopaʻa ʻia e hoʻohuli hou ʻia i kēlā me kēia pōʻaiapili kaʻina, hoʻemi ka hoʻokūkū kūlohelohe i ka hoʻohui ʻana.Kākoʻo kēia ʻenehana loea i nā hale waihona puke heluhelu hoʻokahi a me nā hale waihona puke hoʻokahi, e mālama ana i nā ʻano hana genomic.Hiki i ka Illumina sequencing ke kiʻekiʻe a me ka pololei ke hoʻonoho iā ia ma ke ʻano he pōhaku kihi i ka noiʻi genomics, e hāʻawi i ka mana i nā ʻepekema e wehe i nā mea paʻakikī o nā genomes me nā kikoʻī like ʻole a me ka maikaʻi.
ʻO DNBSEQ, i hoʻomohala ʻia e BGI, ʻo ia kekahi ʻenehana NGS hou i hoʻokō i ka hoʻohaʻahaʻa ʻana i nā kumukūʻai sequencing a hoʻonui i ka throughput.ʻO ka hoʻomākaukau ʻana i nā hale waihona puke DNBSEQ e pili ana i ka ʻāpana DNA, ka hoʻomākaukau ʻana i ka ssDNA a me ka hoʻonui ʻia ʻana o ka pōʻai no ka loaʻa ʻana o nā DNA nanoballs (DNB).Hoʻouka ʻia kēia mau mea ma luna o kahi ʻili paʻa a hoʻopaʻa ʻia e ka hui ʻana o Probe-Anchor Synthesis (cPAS).
Hoʻomaʻamaʻa kā mākou lawelawe hoʻonohonoho hale waihona puke i nā mea kūʻai aku i ka hoʻomākaukau ʻana i kā lākou sequencing hale waihona puke mai nā kumu like ʻole (mRNA, whole genome, amplicon, a me nā mea ʻē aʻe).A laila, hiki ke hoʻouna ʻia kēia mau hale waihona puke i kā mākou mau kikowaena sequencing no ka hoʻokele maikaʻi ʻana a me ka hoʻonohonoho ʻana ma Illumina a i ʻole BGI platform.
