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BSA
Loaʻa i ka paena Bulked Segregant Analysis kahi ʻano hoʻohālikelike maʻamau hoʻokahi a me ka nānā ʻana kiʻekiʻe me ka hoʻonohonoho hoʻonohonoho maʻamau.ʻO ka BSA kahi ʻenehana i hoʻohana ʻia e ʻike koke i nā hōʻailona genetic pili phenotype.Aia i loko o ke kaʻina hana nui o BSA: 1. ke koho ʻana i ʻelua pūʻulu kanaka me nā phenotypes kūʻē loa;2. ka hoʻohui ʻana i ka DNA, RNA a i ʻole SLAF-seq (Hoʻokumu ʻia e Biomarker) o kēlā me kēia kanaka e hana i ʻelua nui o ka DNA;3. ka ʻike ʻana i nā kaʻina like ʻole e pili ana i ka genome kuhikuhi a i ʻole ma waena, 4. wānana i nā wahi i hoʻopili ʻia e ka moho e ED a me SNP-index algorithm;5. Ka nānā 'ana a me ka ho'onui 'ana i nā genes ma nā wahi moho, a me nā mea 'ē a'e. Loa'a nō ho'i ka 'eli 'ana i ka 'ikepili me ka nānā 'ana i ka genetic marker a me ka ho'olālā kumu.
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Amplicon (16S/18S/ITS)
Hoʻokumu ʻia ka paepae Amplicon (16S/18S/ITS) me nā makahiki o ka ʻike ʻana i ka noiʻi papahana microbial diversity, kahi i hoʻopaʻa ʻia i ka nānā ʻana maʻamau a me ka loiloi pilikino: uhi ʻia ka loiloi kumu i ka ʻike loiloi kumu o ka noiʻi microbial o kēia manawa, waiwai a piha ka ʻikepili. a hōʻike ʻia nā hopena o ka nānā ʻana ma ke ʻano o nā hōʻike papahana;He ʻokoʻa ka ʻike o ka loiloi pilikino.Hiki ke koho ʻia nā laʻana a hiki ke hoʻonohonoho pono ʻia nā ʻāpana e like me ka hōʻike loiloi kumu a me ke kumu noiʻi, e ʻike ai i nā koi pilikino.ʻO ka ʻōnaehana hana Windows, maʻalahi a wikiwiki.
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Nā Genetics Evolutionary
ʻO ka Evolutionary genetics kahi lawelawe hoʻonohonoho paʻa i hoʻolālā ʻia no ka hāʻawi ʻana i ka wehewehe piha ʻana i ka ʻike evolutionary o nā mea i hāʻawi ʻia e pili ana i nā ʻano like ʻole, me nā SNP, InDels, SV a me CNV.Hāʻawi ia i nā kānana kumu a pau e pono ai no ka wehewehe ʻana i nā loli evolutionary a me nā hiʻohiʻona genetic o nā heluna kanaka, e like me ke ʻano o ka heluna kanaka, ka ʻokoʻa ʻokoʻa, ka pilina phylogeny, a pēlā aku.
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Hoʻohālikelike Genomics
ʻO ka hoʻohālikelike ʻana i nā genomic ʻo ia hoʻi ka hoʻohālikelike ʻana i nā kaʻina genome piha a me nā hale o nā ʻano ʻano like ʻole.Ke manaʻo nei kēia aʻo ʻana i ka hōʻike ʻana i ka ulu ʻana o nā ʻano mea, ka hana gene, ka ʻōnaehana hoʻoponopono gene ma ka pae genome ma ka ʻike ʻana i nā ʻano hana a me nā mea i mālama ʻia a hoʻokaʻawale ʻia ma nā ʻano like ʻole.ʻO ka haʻawina genomics hoʻohālikelike maʻamau e pili ana i nā loiloi i ka ʻohana gene, ka hoʻomohala ʻana i ka evolutionary, ka duplication genome holoʻokoʻa, ke kaomi koho, etc.
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Nā Genetics Evolutionary
Hoʻokumu ʻia ka heluna kanaka a me ka evolutionary genetic analysis platform ma ke kumu o ka ʻike nui i hōʻiliʻili ʻia i loko o ka hui BMK R&D no nā makahiki.He mea hoʻohana pono ia no ka poʻe noiʻi ʻaʻole nui i ka bioinformatics.Hāʻawi kēia kahua i ka loiloi kumu e pili ana i nā genetics evolutionary e pili ana i ka hana ʻana i nā kumulāʻau phylogenetic, linkage disequilibrium analysis, genetic diversity assessment, selective sweep analysis, kinship analysis, PCA, population structure analysis, etc.
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ʻO ka Genome Assembly ma muli o Hi-C
He ʻano hana ʻo Hi-C no ka hopu ʻana i ka hoʻonohonoho chromosome ma o ka hoʻohui ʻana i nā pilina pili pili kokoke a me ke kaʻina hana kiʻekiʻe.ʻO ka ikaika o kēia mau pilina i manaʻo ʻia ua pili maikaʻi ʻia me ka mamao kino ma nā chromosomes.No laila, hiki i ka ʻikepili Hi-C ke alakaʻi i ka hui ʻana, ka hoʻonohonoho ʻana a me ka hoʻonohonoho ʻana o nā kaʻina i hui ʻia i loko o kahi kikoʻī genome a hoʻopaʻa i kēlā mau mea ma kekahi helu o nā chromosomes.Hāʻawi kēia ʻenehana i kahi hui genome pae chromosome me ka loaʻa ʻole o ka palapala ʻāina hoʻokumu kanaka.Pono kēlā me kēia genome i kahi Hi-C.
Papahana: Illumina NovaSeq Platform / DNBSEQ
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Mea kanu/holoholona De Novo Genome Sequencing
De NovoʻO ke kaʻina hana e pili ana i ke kūkulu ʻana i ka genome holoʻokoʻa o kekahi ʻano me ka hoʻohana ʻana i nā ʻenehana sequencing, e laʻa me PacBio, Nanopore, NGS, etc., me ka loaʻa ʻole o kahi genome kuhikuhi.ʻO ka hoʻomaikaʻi maikaʻi ʻana i ka lōʻihi heluhelu o nā ʻenehana sequencing ʻekolu ua lawe mai i nā manawa hou i ka hui ʻana i nā genomes paʻakikī, e like me nā mea me ka heterozygosity kiʻekiʻe, ka ratio kiʻekiʻe o nā ʻāpana repetitive, polyploids, etc. ka hoʻoholo ʻana i nā mea hana hou, nā ʻāpana me nā mea ʻokoʻa GC a me nā wahi paʻakikī ʻē aʻe.
Papahana: PacBio Sequel II / Nanopore PromethION P48/ Illumina NovaSeq Platform
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ʻO ke Kaʻina Exome Ke kanaka holoʻokoʻa
Manaʻo ʻia ʻo Whole exome sequencing (WES) ma ke ʻano he kumu hoʻolālā hoʻokele waiwai no ka ʻike ʻana i nā mutation kumu maʻi.ʻOiai ʻo nā exons wale nō e lawe ma kahi o 1.7% o ka genome holoʻokoʻa, hōʻike pololei ia i ka ʻike o nā hana protein holoʻokoʻa.I loko o ka genome kanaka, ua hōʻike ʻia ʻoi aku ma mua o 85% o nā hoʻololi e pili ana i nā maʻi i loaʻa i ka ʻāina coding protein.
Hāʻawi ʻo BMKGENE i nā lawelawe hoʻokaʻina exome holoʻokoʻa kanaka piha a maʻalahi me nā hoʻolālā hopu exon like ʻole i loaʻa e hoʻokō i nā pahuhopu noiʻi like ʻole.
Papahana: Illumina NovaSeq Platform
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ʻO ke kaʻina o nā ʻāpana ʻāpana kikoʻī i hoʻonui ʻia (SLAF-Seq)
ʻO ka genotyping kiʻekiʻe, ʻoi aku ka nui o ka heluna kanaka, he hana koʻikoʻi ia i ka noiʻi ʻana i ka hui genetic, e hāʻawi ana i ke kumu genetic no ka ʻike ʻana i nā ʻano hana, ka loiloi evolutionary, etc. ) ua hoʻokomo ʻia e hōʻemi i ke kumukūʻai kaʻina no kēlā me kēia laʻana, ʻoiai e mālama pono i ka hana kūpono ma ka ʻike ʻana i ka hōʻailona genetic.Loaʻa ʻia kēia ma ka unuhi ʻana i ka ʻāpana palena i loko o ka laulā nui i hāʻawi ʻia, ka mea i kapa ʻia ka hale waihona hōʻike hōʻemi (RRL).ʻO ka kikoʻī-locus amplified fragment sequencing (SLAF-Seq) kahi hoʻolālā i hoʻomohala ponoʻī no ka SNP genotyping me ka ʻole a i ʻole ka genome kuhikuhi.
Papahana: Illumina NovaSeq Platform -
Illumina a me BGI
ʻO ka ʻenehana Sequencing Illumina, i hoʻokumu ʻia ma Sequencing by Synthesis (SBS), he hana hou NGS i kākoʻo ʻia i ka honua, kuleana no ka hoʻomohala ʻana ma luna o 90% o ka ʻikepili sequencing o ka honua.ʻO ke kumumanaʻo o SBS e pili ana i ke kiʻi ʻana i ka fluorescently labeled reversible terminator i ka hoʻohui ʻia ʻana o kēlā me kēia dNTP, a ma hope i hoʻokaʻawale ʻia e ʻae i ka hoʻohui ʻana i ka waihona aʻe.Me nā dNTP i hoʻopaʻa ʻia e hoʻohuli hou ʻia i kēlā me kēia pōʻaiapili kaʻina, hoʻemi ka hoʻokūkū kūlohelohe i ka hoʻohui ʻana.Kākoʻo kēia ʻenehana loea i nā hale waihona puke heluhelu hoʻokahi a me nā hale waihona puke hoʻokahi, e mālama ana i nā ʻano hana genomic.Hiki i ka Illumina sequencing ke kiʻekiʻe a me ka pololei ke hoʻonoho iā ia ma ke ʻano he pōhaku kihi i ka noiʻi genomics, e hāʻawi i ka mana i nā ʻepekema e wehe i nā mea paʻakikī o nā genomes me nā kikoʻī like ʻole a me ka maikaʻi.
ʻO DNBSEQ, i hoʻomohala ʻia e BGI, ʻo ia kekahi ʻenehana NGS hou i hoʻokō i ka hoʻohaʻahaʻa ʻana i nā kumukūʻai sequencing a hoʻonui i ka throughput.ʻO ka hoʻomākaukau ʻana i nā hale waihona puke DNBSEQ e pili ana i ka ʻāpana DNA, ka hoʻomākaukau ʻana i ka ssDNA a me ka hoʻonui ʻia ʻana o ka pōʻai no ka loaʻa ʻana o nā DNA nanoballs (DNB).Hoʻouka ʻia kēia mau mea ma luna o kahi ʻili paʻa a hoʻopaʻa ʻia e ka hui ʻana o Probe-Anchor Synthesis (cPAS).
Hoʻomaʻamaʻa kā mākou lawelawe hoʻonohonoho hale waihona puke i nā mea kūʻai aku i ka hoʻomākaukau ʻana i kā lākou sequencing hale waihona puke mai nā kumu like ʻole (mRNA, whole genome, amplicon, a me nā mea ʻē aʻe).A laila, hiki ke hoʻouna ʻia kēia mau hale waihona puke i kā mākou mau kikowaena sequencing no ka hoʻokele maikaʻi ʻana a me ka hoʻonohonoho ʻana ma Illumina a i ʻole BGI platform.
