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Ke Kaʻina Metagenomic -NGS
ʻO ka Metagenome e pili ana i ka hōʻiliʻili ʻana o ka nui o nā mea moʻo o kahi kaiāulu hui o nā mea ola, e like me ka metagenome kaiapuni, metagenome kanaka, a me nā mea ʻē aʻe.ʻO ka metagenomic sequencing he mea hana molecular i hoʻohana ʻia e kālailai i nā mea genomic hui ʻia i unuhi ʻia mai nā laʻana kaiapuni, e hāʻawi ana i ka ʻike kikoʻī i ka ʻano like ʻole a me ka nui o nā ʻano, ke ʻano o ka heluna kanaka, ka pilina phylogenetic, nā genes hana a me ka pilina pili me nā mea kaiapuni.
Papahana:ʻO Illumina NovaSeq Platform
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ʻO ke Kaʻina Metagenomic-Nanopore
ʻO ka Metagenomics kahi mea hana molekala i hoʻohana ʻia e kālailai i nā mea genomic hui pū ʻia i unuhi ʻia mai nā laʻana kaiapuni, e hāʻawi ana i ka ʻike kikoʻī i ka ʻano like ʻole a me ka nui o nā ʻano, ka hoʻonohonoho ʻana o ka heluna kanaka, ka pilina phylogenetic, nā genes hana a me ka ʻoihana correlation me nā mea kaiapuni, a pēlā aku. i nā haʻawina metagenomic.ʻO kāna hana koʻikoʻi i ka lōʻihi heluhelu i hoʻonui nui ʻia i ke kahawai metagenomic analysis, ʻoi aku ka metagenome hui.ʻO ka loaʻa ʻana o ka pono o ka heluhelu-lōʻihi, hiki i ka Nanopore-based metagenomic study ke hoʻokō i ka hui mau ʻana i ka hoʻohālikelike ʻana me nā metagenomics pana-gun.Ua paʻi ʻia ʻo Nanopore-based metagenomics i hoʻopuka maikaʻi i nā genomes bacterial piha a pani ʻia mai nā microbiomes (Moss, EL, et. al,Nature Biotech, 2020)
Papahana:Nanopore PromethION P48
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16S/18S/ITS Amplicon Sequencing-PacBio
ʻO ka subunit ma 16S a me 18S rRNA i loaʻa nā ʻāpana ʻelua i mālama nui ʻia a me nā ʻāpana hyper-variable he manamana lima molekala kūpono no ka ʻike ʻana i nā mea ola prokaryotic a me eukaryotic.Ma ka hoʻohana ʻana i ka hoʻonohonoho ʻana, hiki ke hoʻopaʻa ʻia kēia mau amplicons ma muli o nā ʻāpana i mālama ʻia a hiki ke hōʻike piha ʻia nā ʻāpana hyper-variable no ka ʻike microbial e hāʻawi ana i nā haʻawina e pili ana i ka nānā ʻana i ka microbial diversity analysis, taxonomy, phylogeny, etc. ) ka hoʻonohonoho ʻana o ka paepae PacBio hiki ke loaʻa nā heluhelu lōʻihi pololei loa, hiki ke uhi i nā amplicons lōʻihi piha (ma kahi o 1.5 Kb).ʻO ka ʻike ākea o ke kahua genetic i hoʻomaikaʻi nui i ka hoʻonā ʻana i ka hōʻike ʻana i nā ʻano i loko o ke kaiāulu bacteria a i ʻole nā haʻo.
Papahana:PacBio Sequel II
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16S/18S/ITS Amplicon Sequencing-NGS
16S/18S/ITS amplicon sequencing e hōʻike ana i ka phylogeny, taxonomy, a me ka nui o nā ʻano mea i loko o kahi kaiāulu microbial ma o ka noiʻi ʻana i nā huahana PCR o nā hōʻailona genetic housekeeping i loaʻa nā ʻāpana kamaʻilio nui a me nā ʻāpana hypervariable.ʻO ka hoʻokomo ʻana i kēia mau manamana lima molekala maikaʻi e Woeses et al, (1977) e hoʻoikaika i ka hoʻokaʻawale ʻana i ka microbiome profiling.ʻO ke kaʻina o 16S (bacteria), 18S (fungi) a me Internal transcribed spacer (ITS, fungi) hiki ke ʻike i nā ʻano nui ʻelua a me nā ʻano mea ʻike ʻole ʻia.Ua lilo kēia ʻenehana i mea hoʻohana ākea a me ka mea nui i ka ʻike ʻana i ka haku mele microbial ʻokoʻa i nā ʻano like ʻole, e like me ka waha o ke kanaka, nā ʻōpū, nā feces, etc.
Papahana:ʻO Illumina NovaSeq Platform
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ʻO ka hoʻopaʻa ʻana i ka genome holoʻokoʻa i ka maʻi bacteria a me ka fungal
He mea paahana koʻikoʻi ka hoʻopaʻa ʻana i ka genome holoʻokoʻa bacteria a fungal no ka hoʻopau ʻana i nā genomes o nā huakini a me nā haʻani i ʻike ʻia, a me ka hoʻohālikelike ʻana i nā genomes lehulehu a i ʻole ka palapala ʻana i nā genomes o nā mea ola hou.He mea koʻikoʻi ka hoʻonohonoho ʻana i nā genomes holoʻokoʻa o ka hua bacteria a me nā haʻi i mea e hana ai i nā genome kuhikuhi pololei, e hana i ka ʻike microbial a me nā haʻawina genome hoʻohālikelike.
Papahana: Illumina NovaSeq Platform
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Hoʻokaʻina Metatranscriptome
ʻO ka metatranscriptome sequencing e hōʻike ana i ka hōʻike gene o nā microbes (ʻo nā eukaryotes a me nā prokaryotes) i loko o nā wahi kūlohelohe (ʻo ia hoʻi ka lepo, ka wai, ke kai, nā feces, a me ka ʻōpū.). o nā ʻano, ka loiloi hoʻonui hana o nā ʻano genes i hōʻike ʻia, a me nā mea hou aku.
Papahana: Illumina NovaSeq Platform
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Fungal Genome
Hāʻawi ʻo Biomarker Technologies i ka loiloi genome, genome maikaʻi a me ka genome pene-complete o ka fungal ma muli o ka pahuhopu noiʻi kikoʻī.Hiki ke hoʻokō ʻia ke kaʻina o Genome, ka hui ʻana a me ka hōʻike hana ma o ka hoʻohui ʻana i ke kaʻina o ka hanauna aʻe + ʻO ke kaʻina o ka hanauna ʻekolu e hoʻokō i ka hui genome kiʻekiʻe.Hiki ke hoʻohana ʻia ka ʻenehana Hi-C e hoʻomaʻamaʻa i ka hui genome ma ka pae chromosome.
Papahana:PacBio Sequel II
Nanopore PromethION P48
ʻO Illumina NovaSeq Platform
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ʻO Genome Paʻa ʻo Bacteria
Hāʻawi ʻo Biomarker Technologies i ka lawelawe sequencing ma ke kūkulu ʻana i ka genome piha o ka bacteria me ka ʻole o ke āpau.ʻO ke kaʻina hana nui o ka bacteria e hoʻopau ana i ka hana genome e pili ana i ke kaʻina o ka hanauna ʻekolu, ka hui ʻana, ka annotation hana a me ka loiloi bioinformatic holomua e hoʻokō nei i nā pahuhopu noiʻi kikoʻī.ʻO ka hōʻike piha ʻana o ka genome bacteria e hāʻawi i ka mana i ka hōʻike ʻana i nā mīkini kumu i lalo o kā lākou mau kaʻina olaola, hiki ke hāʻawi i kahi kuhikuhi waiwai no nā noiʻi genomic i nā ʻano eukaryotic kiʻekiʻe.
Papahana:Nanopore PromethION P48 + Illumina NovaSeq Platform
PacBio Sequel II
