-
ʻO Chromatin Immunoprecipitation Sequencing (ChIP-seq)
Hāʻawi ʻo ChIP-Seq i ka genome-wide profiling o nā pahuhopu DNA no ka hoʻololi ʻana i ka histone, nā kumu transcription, a me nā protein e pili ana i ka DNA.Hoʻohui ia i ke koho ʻana o ka chromatin immuno-precipitation (ChIP) e hoʻihoʻi i nā paʻakikī protein-DNA kikoʻī, me ka mana o ka sequencing hanauna hou (NGS) no ke kaʻina hana kiʻekiʻe o ka DNA i hoʻihoʻi ʻia.Hoʻohui ʻia, no ka loaʻa ʻana o nā paʻina protein-DNA mai nā cell ola, hiki ke hoʻohālikelike ʻia nā wahi paʻa i nā ʻano cell like ʻole a me nā ʻiʻo, a i ʻole ma lalo o nā kūlana like ʻole.Loaʻa nā noi mai ka hoʻoponopono transcriptional i nā ala hoʻomohala i nā mīkini maʻi a ma waho.
Papahana: Illumina NovaSeq Platform
-
ʻO ke kaʻina hana bisulfite genome holoʻokoʻa
ʻO ka methylation DNA ma ka pae ʻelima o ka cytosine (5-mC) he mana koʻikoʻi i ka hōʻike ʻana o ka gene a me ka hana kelepona.Hoʻopili ʻia nā ʻano methylation maʻamau me kekahi mau maʻi a me nā maʻi, e like me ke kanesa.Ua lilo ʻo WGBS i kumu gula no ke aʻo ʻana i ka genome-wide methylation ma ka hoʻonā kumu hoʻokahi.
Papahana: Illumina NovaSeq Platform
-
ʻIke no ka Transposase-Accessible Chromatin me ke Kiʻekiʻe Throughput Sequencing (ATAC-seq)
ʻO ATAC-seq kahi ala hoʻokaʻina kiʻekiʻe no ka nānā ʻana i ka hiki ke komo i ka chromatin genome-ākea, he mea nui ia no ka mana epigenetic honua o ka hōʻike gen.Hoʻokomo ʻia nā mea hoʻopili sequencing i nā ʻāpana chromatin ākea e ka hyperactive Tn5 transposase.Ma hope o ka hoʻonui ʻia ʻana o PCR, kūkulu ʻia kahi waihona sequencing.Hiki ke loaʻa nā ʻāpana chromatin hāmama a pau ma lalo o kahi kūlana manawa-manawa kikoʻī, ʻaʻole i kaupalena ʻia i nā wahi paʻa o kahi mea transcription, a i ʻole kekahi ʻāpana histone i hoʻololi ʻia.
-
Hoʻemi ʻia ka hoʻohālikelike Bisulfite Sequencing (RRBS)
ʻO ka noiʻi DNA methylation he kumuhana wela i ka noiʻi maʻi, a pili pili i ka hōʻike gene a me nā ʻano pheno-typic.ʻO RRBS kahi ala pololei, kūpono a me ka waiwai no ka noiʻi DNA methylation.ʻO ka hoʻonui ʻia ʻana o ka mea hoʻolaha a me nā ʻāina mokupuni CpG e ka enzymatic cleavage (Msp I), i hui pū ʻia me ka Bisulfite sequencing, hāʻawi i ka ʻike methylation DNA kiʻekiʻe.
Papahana: Illumina NovaSeq Platform
