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PacBio-Ka lōʻihi piha 16S/18S/ITS Amplicon Sequencing
Hoʻokumu ʻia ka paepae Amplicon (16S/18S/ITS) me nā makahiki o ka ʻike ʻana i ka noiʻi papahana microbial diversity, kahi i hoʻopaʻa ʻia i ka nānā ʻana maʻamau a me ka loiloi pilikino: uhi ʻia ka loiloi kumu i ka ʻike loiloi kumu o ka noiʻi microbial o kēia manawa, waiwai a piha ka ʻikepili. a hōʻike ʻia nā hopena o ka nānā ʻana ma ke ʻano o nā hōʻike papahana;He ʻokoʻa ka ʻike o ka loiloi pilikino.Hiki ke koho ʻia nā laʻana a hiki ke hoʻonohonoho pono ʻia nā ʻāpana e like me ka hōʻike loiloi kumu a me ke kumu noiʻi, e ʻike ai i nā koi pilikino.ʻO ka ʻōnaehana hana Windows, maʻalahi a wikiwiki.
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PacBio-Full-lōʻihi Transcriptome (Non-Reference)
Ke lawe nei i ka ʻikepili hoʻokaʻina Isoform i ka Pacific Biosciences (PacBio) ma ke ʻano he hoʻokomo, hiki i kēia App ke ʻike i nā kaʻina transcript lōʻihi (me ka ʻole o ka hui ʻana).Ma ka palapala ʻana i nā kaʻina holoʻokoʻa kūʻē i ka genome kuhikuhi, hiki ke hoʻopaʻa ʻia nā transcripts e nā genes i ʻike ʻia, transcripts, coding regions, etc.ʻO ka loiloi hui pū me ka NGS transcriptome sequencing data e hiki ai i ka hōʻike kikoʻī a me ka helu pololei ʻana i ka hōʻike ʻana ma ka pae transcript, ka mea e pōmaikaʻi nui ai i ka hōʻike ʻokoʻa a me ka loiloi hana.
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Mea paahana
ʻO BMKCloud kahi papahana bioinformatic alakaʻi e hāʻawi ana i ka hopena hoʻokahi no nā papahana genomic, kahi i hilinaʻi nui ʻia e nā mea noiʻi ma nā ʻano ʻano like ʻole me ka lāʻau lapaʻau, ka mahiʻai, ka nohona, a me nā mea ʻē aʻe. , punawai helu, waihona lehulehu, bioinformtic online courses, etc. BMKCloud he mau mea hana bioinformatic i hoohana pinepine ia e like me ka gene annotation, evolutionary genetic tools, ncRNA, data quality control, assembly, alignment, data extraction, mutations, statistics, figure generator, sequence. kālailai, etc.
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RNA liʻiliʻi
ʻO nā RNA liʻiliʻi he ʻano pōkole non-coding RNA me ka lōʻihi o 18-30 nt, me ka miRNA, siRNA a me ka piRNA.Ua hōʻike nui ʻia kēia mau RNA liʻiliʻi e pili ana i nā kaʻina hana ola like ʻole e like me ka hoʻohaʻahaʻa ʻana o ka mRNA, ka pāpā ʻana i ka unuhi, ka hoʻokumu ʻana o heterochromatin, a me nā mea ʻē aʻe. ʻO ka papa hana hoʻopaʻa ʻana kaʻina hana maʻamau a me ka ʻimi ʻikepili kiʻekiʻe.Ma ke kumu o ka ʻikepili RNA-seq, hiki i ka loiloi maʻamau ke hoʻokō i ka ʻike miRNA a me ka wānana, ka wānana gene target miRNA, annotation a me ka nānā ʻana i ka ʻōlelo.Hiki i ka loiloi holomua ka huli ʻana a me ka unuhi ʻana o ka miRNA, ka hana kiʻi Venn, ka miRNA a me ka kūkulu ʻana i ka ʻoihana gene.
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NGS-WGS (Illumina/BGI)
ʻO NGS-WGS kahi kahua hōʻano hou genome re-sequencing ana, i hoʻomohala ʻia ma ke kumu o ka ʻike waiwai ma Biomarker Technologies.Hāʻawi kēia paepae maʻalahi i ka hoʻouna wikiwiki ʻana i kahi kaila hana hoʻopili i hoʻohui ʻia ma ka hoʻonohonoho ʻana i kekahi mau ʻāpana kumu, i kūpono no ka ʻikepili sequencing DNA i hana ʻia mai ka paepae Illumina a me BGI sequencing platform.Hoʻokomo ʻia kēia kahua ma luna o ka server computing hana kiʻekiʻe, e hāʻawi ana i ka mana o ka nānā ʻana i ka ʻikepili kūpono loa i ka manawa pōkole loa.Loaʻa ka mining data pilikino ma ke kumu o ka nānā ʻana maʻamau, me ka huli ʻana i nā gene mutated, PCR primer design, etc.
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mRNA(Hōʻike)
ʻO Transcriptome ka loulou ma waena o ka ʻike genetic genomic a me ka proteome o ka hana biological.ʻO ka hoʻoponopono pae transcription ka mea koʻikoʻi a ʻo ke ʻano hoʻoponopono i aʻo nui ʻia o nā meaola.Hiki i ka transcriptome sequencing ke hoʻonohonoho i ka transcriptome i kēlā me kēia manawa i ka manawa a i ʻole ma lalo o kekahi kūlana, me ka hoʻoholo pololei i ka nucleotide hoʻokahi. laʻana i nā palapala kikoʻī.
I kēia manawa, ua hoʻohana nui ʻia ka ʻenehana sequencing transcriptome i ka agronomy, ka lāʻau lapaʻau a me nā ʻano noiʻi ʻē aʻe, me ka hoʻoponopono ʻana i nā holoholona a me nā mea kanu, ka hoʻoponopono ʻana i ke kaiapuni, ka launa pū ʻana, ka localization gene, species genetic evolution and tumor and genetic disease detection.
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Metagenomics (NGS)
Hoʻolālā ʻia kēia kahua hoʻopaʻa inoa no ka ʻikepili ʻikepili metagenomic shotgun ma ke kumu o nā makahiki o ka ʻike.Loaʻa iā ia ke kahe hana i hoʻohui ʻia i loko o nā ʻano loiloi metagenomics maʻamau e pono ai e pili ana i ka hoʻoili ʻana i ka ʻikepili, nā haʻawina pae ʻano, nā haʻawina pae hana gene, metagenome binning, a me nā mea ʻē aʻe. , hoʻonohonoho hoʻonohonoho ʻāpana, hana kiʻi pilikino, etc.
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LncRNA
ʻO nā RNA non-coding lōʻihi (lncRNA) kekahi ʻano o nā transcript me ka lōʻihi ma mua o 200 nt, ʻaʻole hiki ke hoʻopaʻa i nā protein.Hōʻike nā hōʻike hōʻuluʻulu e hiki ke hana ka hapa nui o nā lncRNA.Hiki iā mākou ke hōʻike i nā kaʻina lncRNA a me ka hoʻonohonoho ʻana i ka ʻike a alakaʻi iā mākou e ʻike i nā lncRNA me nā hana hoʻoponopono koʻikoʻi.Haʻaheo ʻo BMKCloud e hāʻawi i kā mākou mea kūʻai aku lncRNA sequencing analysis platform e hoʻokō i ka loiloi lncRNA wikiwiki, hilinaʻi a maʻalahi.
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GWAS
ʻO ka Genome-wide association study (GWAS) ka manaʻo e ʻike i nā ʻano ʻano genetic (genotype) e pili ana i nā ʻano kikoʻī (phenotype).Ke noiʻi nei nā haʻawina GWA i nā mea hōʻailona genetic e hele i ka genome holoʻokoʻa o ka heluna nui o nā kānaka a wānana i nā hui genotype-phenotype ma o ka nānā ʻana helu helu ma ka pae heluna kanaka.Hiki ke ʻike i nā ʻano genetic āpau i ka resequencing holoʻokoʻa.I ka hui pū ʻana me ka ʻikepili phenotypic, hiki ke hana ʻia ʻo GWAS e ʻike i nā SNP pili phenotype, QTL a me nā genes moho, e kākoʻo ikaika ana i ka hoʻohua holoholona/mea kanu o kēia wā.ʻO SLAF kahi hoʻolālā hoʻolālā genome maʻalahi i hoʻomohala ʻia, kahi e ʻike ai i nā māka hoʻolaha genome-ākea, SNP.ʻO kēia mau SNP, ma ke ʻano he molecular genetic markers, hiki ke hana ʻia no nā haʻawina hui me nā ʻano i kuhikuhi ʻia.He hoʻolālā kumu kūʻai i ka ʻike ʻana i nā ʻano paʻakikī e pili ana i nā ʻano like ʻole.
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Nanopore Ka lōʻihi lōʻihi
ʻO nā isoforms paʻakikī a me nā ʻano like ʻole i loko o nā mea ola he mau ʻano genetic koʻikoʻi no ka hoʻoponopono ʻana i ka hōʻike gene a me ka ʻokoʻa protein.ʻO ka ʻike pololei ʻana i nā hale transcript ke kumu no ka noiʻi hohonu ʻana i nā ʻano hoʻoponopono hoʻoponopono hōʻike gene.Ua hoʻokō maikaʻi ʻo Nanopore sequencing platform i ka haʻawina transcriptomic i ka pae isoform.Hoʻolālā ʻia kēia kahua hoʻopaʻa ʻana e kālailai i ka ʻikepili RNA-Seq i hana ʻia ma ke kahua Nanopore ma ke kumu o ka genome reference, kahi e loaʻa ai nā loiloi qualitative a me quantitative ma nā pae gene a me nā pae transcripts.
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pōʻai-RNA
ʻO ka Circular RNA(circRNA) kekahi ʻano o ka RNA non-coding, i ʻike ʻia i kēia manawa e pāʻani i ke kuleana koʻikoʻi i nā ʻoihana hoʻoponopono e pili ana i ka hoʻomohala ʻana, ka pale ʻana i ke kaiapuni, a pēlā aku. Hoʻohui ʻia nā ʻaoʻao o ka circRNA e hana i kahi ʻano pōʻai, e hoʻopakele iā lākou mai ka ʻeli ʻana o exonuclease a ʻoi aku ka paʻa ma mua o ka hapa nui o ka RNA linear.Ua ʻike ʻia ʻo CircRNA he mau hana like ʻole i ka hoʻoponopono ʻana i ka hōʻike gene.Hiki i ka CircRNA ke hana ma ke ʻano he ceRNA, nāna e hoʻopaʻa i ka miRNA me ka hoʻokūkū, i kapa ʻia ʻo miRNA sponge.Hāʻawi ka CircRNA sequencing analysis platform i ka hoʻolālā circRNA a me ka nānā ʻana i ka hōʻike, ka wānana pahuhopu a me ka loiloi hui pū me nā ʻano mole RNA ʻē aʻe.
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BSA
Loaʻa i ka paena Bulked Segregant Analysis kahi ʻano hoʻohālikelike maʻamau hoʻokahi a me ka nānā ʻana kiʻekiʻe me ka hoʻonohonoho hoʻonohonoho maʻamau.ʻO ka BSA kahi ʻenehana i hoʻohana ʻia e ʻike koke i nā hōʻailona genetic pili phenotype.Aia i loko o ke kaʻina hana nui o BSA: 1. ke koho ʻana i ʻelua pūʻulu kanaka me nā phenotypes kūʻē loa;2. ka hoʻohui ʻana i ka DNA, RNA a i ʻole SLAF-seq (Hoʻokumu ʻia e Biomarker) o kēlā me kēia kanaka e hana i ʻelua nui o ka DNA;3. ka ʻike ʻana i nā kaʻina like ʻole e pili ana i ka genome kuhikuhi a i ʻole ma waena, 4. wānana i nā wahi i hoʻopili ʻia e ka moho e ED a me SNP-index algorithm;5. Ka nānā 'ana a me ka ho'onui 'ana i nā genes ma nā wahi moho, a me nā mea 'ē a'e. Loa'a nō ho'i ka 'eli 'ana i ka 'ikepili me ka nānā 'ana i ka genetic marker a me ka ho'olālā kumu.
