Nā huahana

Mea kanu/holoholona holoʻokoʻa Genome Sequencing

ʻO ka hoʻonohonoho hou ʻana o ka genome holoʻokoʻa, ʻike ʻia ʻo WGS, hiki ke hōʻike ʻia nā hoʻololi maʻamau a me nā ʻano like ʻole ma ka genome holoʻokoʻa me Single Nucleotide Polymorphism (SNP), Insertion Deletion (InDel), Structure variation (SV), a me Copy Number Variation (CNV). ).Hoʻokumu nā SV i kahi hapa nui o ka waihona hoʻololi ma mua o nā SNP a ʻoi aku ka nui o ka hopena i ka genome, he hopena koʻikoʻi i nā mea ola.ʻO ka heluhelu lōʻihi e hiki ai ke ʻike pololei i nā ʻāpana nui a me nā ʻano paʻakikī no ka mea ʻoi aku ka maʻalahi o ka heluhelu lōʻihi i ka hele ʻana o ka chromosomal ma luna o nā wahi paʻakikī e like me ka tandem repeats, GC/AT-rich regions, a me hyper-variable regions.

Papahana: Illumina, PacBio, Nanopore

Kāhea iā mā˚ou

Nā kikoʻī lawelawe

Ka hopena Demo

Hoʻolaha ʻia

1.Service Pono

ʻO ka ʻike nui i ka hoʻonohonoho genome no ka ʻoi aku o 1000 mau ʻano.

ʻOi aku ma mua o 800 mau hihia i paʻi ʻia me kahi kumu hopena hoʻohui ʻia ma luna o 4000.

ʻO ka loiloi bioinformatics piha e pili ana i ke kelepona ʻana a me ka nānā ʻana i ka hana.

2. Nā kikoʻī lawelawe

Papahana		Hale Waihona Puke	Manaʻo ʻia ka hohonu seq
ʻIlumina		PE 150	No SNP, kelepona ʻo InDel ≥ 10x No SV, CNY kelepona ʻana ≥ 30x
Nanopore		8 kb	No SV, CNY kelepona ≥ 20x
ʻo Pacbio	CCS	15 kb	No SNP, InDel, SV, CNY e kāhea ana ≥ 10x

3. Nā Koina Laʻana

Papahana		Conc.(ng/μL)	Ka nui (ng)	Maemae		ʻO Agarose gel
				OD260/280	OD260/230	1. Hoʻomaʻemaʻe i ka hui nui me ka ʻole a i kaupalena ʻiaʻike ʻia ka degradation ma ka gel. 2. ʻAʻole a i kaupalena ʻia ka RNA a i ʻole ka palaka
ʻIlumina		≥1	≥30	-	-
Nanopore		≥30	Ma muli o ka loaʻa ʻana o ka ʻikepili 10μg/cell	1.7-2.2	≥1.5
ʻo Pacbio	CCS	≥50		1.7-2.2	1.8-2.5

4. ʻIkepili Bioinformation

5. Hana Hana Hana

Hāʻawi laʻana

Ka unuhi ʻana o DNA

Kūkulu hale waihona puke

Ke kaʻina ʻana

ʻIkepili ʻikepili

Hāʻawi ʻikepili

Mua: BMKMANU S1000 Spatial Transcriptome

Aʻe: Hoʻokahi-nucleus RNA Sequencing

1) Heluhelu o ka Palapala Kiʻi Genome

Papa 1 Heluhelu o ka hopena palapala

Kiʻi 1 Ka māhele ʻana o ka nui hoʻokomo a me ka uhi heluhelu.

2) ʻIke ʻokoʻa

Kiʻi 2 Heluhelu a me ka hōʻike ʻana o SNP/INDEL/SV ma waena o nā Laʻana

Kiʻi 3 Māhele Genome-ākea o nā vatiations

3) Hōʻike kikoʻī o nā ʻano like ʻole

2019	Nā kamaʻilio kūlohelohe	Hōʻike ka holoʻokoʻa-genome resequencing i ke kumu Brassica napus a me ka genetic loci i komo i kona hoʻomaikaʻi ʻana
2020	PNAS	ʻO ka hoʻomaka ʻana a me ka mōʻaukala home o ka iʻa gula (Carassius auratus)
2021	Mea Puke Biotechnology Journal	Hōʻike i nā genomes o nā ʻano jute ʻelua i kanu ʻia
2021	Mea Puke Biotechnology Journal	ʻO nā pūlima genomic o ka mea kanu a me ka oilseed allopolyploid Brassicajuncea a me ka genetic loci e kāohi ana i ka hōʻiliʻili ʻana o nā glucosinolates
2019	Mea Molekula	ʻO ka hoʻihoʻi ʻana o ka genome holoʻokoʻa o kahi hōʻiliʻili honua āpau o ka komo ʻana i ka rapeseed e hōʻike ana i ke kumu genetic o kā lākou ecotype divergence
2022	ʻImi Horticulture	Hāʻawi ka ʻimi ʻana i ka hui ʻana o Genome-wide i nā ʻike molekala i ke ʻano kūlohelohe o ka nui o ka hua melon
2021	Nūpepa o ka hoʻokolohua Botany	ʻIke ʻia ka haʻawina hui ākea Genome i nā ʻano like ʻole o GhSAD1 e hāʻawi ana i ka hoʻomanawanui anu i ka pulupulu
2021	Nūpepa o ka hoʻokolohua Botany	ʻO ke aʻo ʻana o ka hui holoʻokoʻa Genome a me ka hoʻohālikelike transcriptome e hōʻike ana i ka QTL hou a me nā genes moho e hoʻomalu i ka nui o ka petal i ka rapeseed.