GENOME EVOLUTION
Genome De novo
Ahahui|Hoʻoholo i ka wahine Ua hāʻawi ʻia nā hana hoʻonohonoho holoʻokoʻa a me nā lawelawe bioinformatic ʻāpana e Biomarker Techonologies.
ʻĀpana
“ʻO ka hiʻohiʻona hiʻohiʻona o nā seadragons e pili ana i nā mea hoʻopili e like me ka lau, ka waha tubular ʻole, a me ka hāpai kāne kāne e pili ana i ka hoʻoulu ʻana o nā hua manu ma kahi "brood patch."We de novo-sequenced genomes kāne a wahine o ke kai maʻamau (Phyllopteryx taeniolatus) a me kona ʻano pili pili, ka alligator pipefish (Syngnathoides biaculeatus).Hōʻike ʻia nā moʻolelo moʻolelo mai kahi mea hou evolutionary, nā mea hoʻohui like me ka lau, ua hoʻopili ʻia kahi pūʻulu o nā genes i komo i ka hoʻomohala ʻana a me ka hoʻonui ʻana i nā palapala kope no ka hoʻoponopono ʻana i ka ʻiʻo a me nā genes pale pale.ʻO nā mutants zebrafish no scpp5, ka mea i nalowale ma nā syngnathids a pau, ua ʻike ʻia ka nele a i ʻole ka deformed o nā niho pharyngeal, e kākoʻo ana i ke kuhiakau ua kōkua ka nalowale o ka scpp5 i ka nalowale o nā niho i nā syngnathids.Ua ʻike ʻia kahi locus e hoʻoholo ana i ka wahine e hoʻopili ana i kahi gene amhr2y kikoʻī kāne i kaʻana like ʻia e ka seadragon maʻamau a me ka alligator pipefish. ”
Nā mea a me nā ʻano hana:
Nā mea waiwai
Fʻo kā mākou puaʻa kai maʻamau (P. taeniolatus) a me ʻelua iʻa paipu alligator (S. biaculeatus) nā mea hoʻohālike.Ua hoʻohana ʻia ʻelua mau iʻa kai maʻamau (hoʻokahi kāne a hoʻokahi wahine) a me ʻelua iʻa alligator pipefish (hoʻokahi kāne a me hoʻokahi wahine) no ka hoʻonohonoho genome holoʻokoʻa, a ua hoʻohana ʻia ʻelua mau kāne kai maʻamau no ka resequencing genome.
Hoʻolālā Genome Sequencing
270/350 bp hale waihona puke (Hiseq 2500) + Pacbio 20Kb (Sequel) a i ʻole Nanopore 30 Kb (MinION) + Hi-C (NovaSeq 6000) .sequencing, a me ʻelua mau kāne maʻamau seadragon kanaka i hoʻohana ʻia no ka genome resequencing.
Hoʻokaʻina Kakau
Fa i ʻole, ʻo ka lolo, ka maka, ka gill, ke akepaʻa, ka puʻuwai, ka puʻupaʻa, ka ʻōpū, ka ʻōpū, ka ʻiʻo, ka ʻili, ka ʻili, nā mea e like me ka lau, ka ʻiʻo, a me ka ovary.
ʻO ka papahana hui Genome de novo
Canu (Hoʻoponopono) + WTDBG2 (Ahui) + Pilon (Polani) + Lachesis (Hi-C).
Nā Hualoaʻa Nui
Fig. 1. Nā hiʻohiʻona nui o ka iʻa kai maʻamau (P. taeniolatus) a me ka iʻa paipa alligator (S. biaculeatus) a me ko lākou kūlana phylogenetic.
Fig. 2. Morphology and genetic makeup of the lau-like appendages in the common seadragon (P. taeniolatus).
Fig. 3. Putative gene determination gene in the common seadragon (P. taeniolatus) and the alligator pipefish (S. biaculeatus).
Fig. 4. Pharyngeal niho phenotypes in zebrafish scpp5 homozygous mutants.
Ka manawa hoʻouna: Jan-08-2022