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GENOME EVOLUTION

Genome De novo
Ahahui|Hoʻoholo i ka wahine Ua hāʻawi ʻia nā hana hoʻonohonoho holoʻokoʻa a me nā lawelawe bioinformatic ʻāpana e Biomarker Techonologies.

ʻĀpana

ʻO ka hiʻohiʻona hiʻohiʻona o nā seadragons e pili ana i nā mea hoʻopili e like me ka lau, ka waha tubular ʻole, a me ka hāpai kāne kāne e pili ana i ka hoʻoulu ʻana o nā hua manu ma kahi "brood patch."We de novo-sequenced genomes kāne a wahine o ke kai maʻamau (Phyllopteryx taeniolatus) a me kona ʻano pili pili, ka alligator pipefish (Syngnathoides biaculeatus).Hōʻike ʻia nā moʻolelo moʻolelo mai kahi mea hou evolutionary, nā mea hoʻohui like me ka lau, ua hoʻopili ʻia kahi pūʻulu o nā genes i komo i ka hoʻomohala ʻana a me ka hoʻonui ʻana i nā palapala kope no ka hoʻoponopono ʻana i ka ʻiʻo a me nā genes pale pale.ʻO nā mutants zebrafish no scpp5, ka mea i nalowale ma nā syngnathids a pau, ua ʻike ʻia ka nele a i ʻole ka deformed o nā niho pharyngeal, e kākoʻo ana i ke kuhiakau ua kōkua ka nalowale o ka scpp5 i ka nalowale o nā niho i nā syngnathids.Ua ʻike ʻia kahi locus e hoʻoholo ana i ka wahine e hoʻopili ana i kahi gene amhr2y kikoʻī kāne i kaʻana like ʻia e ka seadragon maʻamau a me ka alligator pipefish. 

Nā mea a me nā ʻano hana:

Nā mea waiwai

Fʻo kā mākou puaʻa kai maʻamau (P. taeniolatus) a me ʻelua iʻa paipu alligator (S. biaculeatus) nā mea hoʻohālike.Ua hoʻohana ʻia ʻelua mau iʻa kai maʻamau (hoʻokahi kāne a hoʻokahi wahine) a me ʻelua iʻa alligator pipefish (hoʻokahi kāne a me hoʻokahi wahine) no ka hoʻonohonoho genome holoʻokoʻa, a ua hoʻohana ʻia ʻelua mau kāne kai maʻamau no ka resequencing genome.

Hoʻolālā Genome Sequencing

270/350 bp hale waihona puke (Hiseq 2500) + Pacbio 20Kb (Sequel) a i ʻole Nanopore 30 Kb (MinION) + Hi-C (NovaSeq 6000) .sequencing, a me ʻelua mau kāne maʻamau seadragon kanaka i hoʻohana ʻia no ka genome resequencing.

Hoʻokaʻina Kakau

Fa i ʻole, ʻo ka lolo, ka maka, ka gill, ke akepaʻa, ka puʻuwai, ka puʻupaʻa, ka ʻōpū, ka ʻōpū, ka ʻiʻo, ka ʻili, ka ʻili, nā mea e like me ka lau, ka ʻiʻo, a me ka ovary.

ʻO ka papahana hui Genome de novo

Canu (Hoʻoponopono) + WTDBG2 (Ahui) + Pilon (Polani) + Lachesis (Hi-C).

Nā Hualoaʻa Nui

Fig.-1.-Nā hiʻohiʻona-o-ka-seadragon-maʻamau-P.-taeniolatus-a-ka-alligator-pipefish-S

Fig. 1. Nā hiʻohiʻona nui o ka iʻa kai maʻamau (P. taeniolatus) a me ka iʻa paipa alligator (S. biaculeatus) a me ko lākou kūlana phylogenetic.

Fig.-2.-Morphology-and-genetic-makeup-o-ka-lau-like-appendages-i-ka-seadragon-maʻamau-P

Fig. 2. Morphology and genetic makeup of the lau-like appendages in the common seadragon (P. taeniolatus).

Fig.-3.-Putative-sex-determination-gene-in-the-common-seadragon-P.-taeniolatus-and-the-alligator-pipefish-S.-biaculeatus

Fig. 3. Putative gene determination gene in the common seadragon (P. taeniolatus) and the alligator pipefish (S. biaculeatus).

Fig.-4

Fig. 4. Pharyngeal niho phenotypes in zebrafish scpp5 homozygous mutants.


Ka manawa hoʻouna: Jan-08-2022

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