I ka makahiki 2021, ua ʻike ʻo BMKGENE i ka 31 De novo genome noiʻi i paʻi maikaʻi ʻia ma nā puke moʻolelo kiʻekiʻe me ka nui o nā mea hopena ma luna o 320. Ua kākau pū ʻia 15 o nā ʻatikala 4 o lākou i kākau pū ʻia ma ke ʻano he mea kākau mua e BMKGENE
Ma hope o ka hoʻomaka ʻana o ka makahiki 2022, ua paʻi ʻia nā ʻatikala noiʻi ʻelua ma ka puke pai "Natural Genetics" a me "Molecular Plant".ʻO lākou, "ʻElua mau haplotypes ʻokoʻa mai kahi genome lychee heterozygous nui e hōʻike ana i nā hanana home kūʻokoʻa no nā cultivars mua a me ka hope o ke oʻo ʻana" (Lychee genome noiʻi, alakaʻi ʻia e College of Horticulture, South China Agricultural university, a me nā hoa hana ʻepekema, i paʻi ʻia ma Natural Genetics. ), a me "Genome sequences o nā ʻano Sitopsis ʻelima o Aegilops a me ke kumu o ka palaoa polyploid B-subgenome" (Five Sitopsis species genome, i alakaʻi ʻia e Profesor Bao Liu ka hui noiʻi o Northeast Normal University.).E nānā pū mākou i kēia mau ʻatikala ʻelua a kaʻana like me kā mākou poʻe heluhelu.
I kēia manawa, e nānā kākou i nā ʻatikala noiʻi maikaʻi loa i paʻi ʻia ma 2021 i kākau pū ʻia e BMK a me kā mākou mau mea hana.
Mea kanu Genome — Nā mea hou ma luna o nā ʻano like ʻole.
1. ʻO kahi hui genome kūlana kiʻekiʻe e hōʻike ana i nā ʻano genomic rye a me nā genes koʻikoʻi agronomically
Mea Hana Hui: Henan Agricultural University
Nūpepa: Natural Genetics
Ka hopena: 38.31
Ma kēia papahana, ua hoʻonohonoho ʻia ka genome o Weining rye, kahi ʻano rai Kina elite.ʻO nā contigs i hui ʻia (7.74 Gb) he 98.47% o ka nui genome i manaʻo ʻia (7.86 Gb), me 93.67% o nā contigs (7.25 Gb) i hāʻawi ʻia i ʻehiku chromosomes.ʻO 90.31% o ka genome i hui pū ʻia.ʻO nā kānana hou aʻe o ka hui ʻo Weining e hoʻomālamalama hou i ka hoʻopili ʻana o ka genome-ākea a me ko lākou hopena i nā genes biosynthesis starch, hoʻonohonoho kino o ka prolamin loci paʻakikī, nā hiʻohiʻona hōʻike gene i lalo o ke ʻano poʻomanaʻo mua a me nā wahi chromosomal pili i ka home a me ka loci i ka rai.Hoʻohiki kēia kaʻina genome e hoʻolōʻihi i nā haʻawina genomic a me ka hānau ʻana i ka rai a me nā mea kanu cereal pili.
2. ʻO Rose me ka ʻole o ka prickle: nā ʻike genomic i pili i ka hoʻololi ʻana i ka wai
Mea Hana Hana: Kunming Institute of Botany, Chinese Academy of Sciences
Nūpepa: National Science Review
Ka hopena: 17.273
Ma kēia pāhana, ua hōʻiliʻili ʻia nā laʻana o 'Basye's Thornless' (BT, he prickle-free cultivar of Rosa wichuraiana), 'Old Blush' (OB, he genotype founder in rose domestication), kā lākou F1 hybrids a me BCF1 i hōʻiliʻili ʻia.A ua hoʻokumu ʻia kahi hui genome reference kiʻekiʻe e ʻike i nā mea genetic e pili ana i ka hoʻomohala ʻana i ka stem prickle.ʻO ka nui genome ma kahi o 530.6 Mb.No ka hōʻoia ʻana i ka maikaʻi o ka genome i hui ʻia, ua mālama ʻia ka nānā ʻana e like me ka hoʻohālikelike palapala genetic, BUSCO, NGS i ka reassembly, ka hoʻohālikelike ʻana me ka haplotype OB, ka hoʻopaʻa ʻana i ka helu kuhi hewa kumu a me ka loiloi waiwai LTR Assembly Index genome-wide (LAI=20.03).Hōʻike kēia noiʻi ʻana i ke ʻano hoʻoilina paʻakikī a me nā ʻano hoʻoponopono o nā stem prickles a hāʻawi iā mākou i kahi kumu a me nā kumuwaiwai hou e aʻo ai i ka biology rose a me kaʻu mau māka molekala e pili ana i nā ʻano koʻikoʻi.
3. Hōʻike ʻo Whole-Genome Sequence o nā Allopolyploid Synthesized i Cucumis i nā ʻike i ka Genome Evolution o Allopolyploidization
Mea Hana Hui: Nanjing Agricultural University
Nūpepa: ʻEpekema Kiʻekiʻe
Ka hopena: 16.801
Ua hōʻike ʻia kēia haʻawina i ka genome kiʻekiʻe o kahi allotetraploid synthetic i loaʻa me ka hoʻohana ʻana i ka interspecific hybridization ma waena o ka kukumba (C. sativus, 2n = 14) a me kāna ʻano pili holoholona hihiu (C. hystrix, 2n = 24) a me ka hoʻopili hou ʻana o ka chromosome, ʻo ia ka mua. allopolyploid synthetic i hoʻopili piha ʻia.Ua hoʻohana ka hui o ka genome i ke kaʻina hana o "PacBio + BioNano + Hi-C + Illumina" sequencing, i hopena i ka nui genome o 530.8Mb a me ka contig N50 = 6.5Mb.Ua hāʻawi ʻia nā heluhelu i 19 pseudochromosomes a me nā subgenomes.Ua hōʻike nā hopena i ka hybridization, ma mua o ka hoʻololi ʻana i ka genome, ke kumu o ka hapa nui o nā loli genomic i nā genomes nuklea a me cp.Ua manaʻo ʻia e hāʻawi ka heterozygosity paʻa iā C. × hytivus me ka hoʻonui ʻana i ke koʻikoʻi.Hāʻawi nā hualoaʻa i nā ʻike hou i ka ulu ʻana o ka polyploidy mea kanu a hāʻawi i kahi hoʻolālā hoʻohua kūpono no nā mea kanu e hiki mai ana.
4. Hoʻohālikelike Genome Analyzes Highlight Transposon Mediated Genome Expansion and the Evolutionary Architecture of 3D Genomic Folding in Cotton
Mea Hana Hui: Huazhong Agricultural University
Nūpepa: Molecular Biology and Evolution
Ka hopena: 16.242
Ua hoʻohana kēia papahana iā Nanopore Sequencing e hōʻuluʻulu i ka genome o ʻekolu ʻano pulupulu ʻo ia hoʻi: Gossypium rotundifolium (K2, nui genome = 2.44Gb, contigN50 = 5.33Mb), G. arboreum (A2, nui genome = 1.62Gb, contigN50 = 11.69Mb), a G. raimondii (D5, nui genome = 0.75Gb, contigN50 = 17.04 Gb).Ma luna o 99% o nā genome ʻekolu i hōʻuluʻulu ʻia ma o Hi-C.ʻO nā hopena o ka nānā ʻana o BUSCO he 92.5%, 93.9% a me 95.4%.ʻO kēia mau helu āpau i hōʻike ʻia ʻo nā genomes hui ʻekolu he kuhikuhi -grade.Hoʻopaʻa ʻia nā kānana genome hoʻohālikelike i nā kikoʻī o ka hoʻonui ʻana i ka TE moʻokūʻauhau e hāʻawi ana i nā ʻokoʻa nui o ka genome.Hōʻike kēia haʻawina i ka hana o ka transposon-mediated genome expansion i ka hoʻomohala ʻana o ka hoʻolālā chromatin kiʻekiʻe i nā mea kanu.
5. ʻO ka hui ʻana o ka Chromosome-scale a me ka nānā ʻana o ka mea kanu biomass Miscanthus lutarioriparius genome
Mea Hana Hui: CAS Center for Excellence in molecular Plant Sciences
Nūpepa: Nature Communications
Ka hopena: 14.912
Ua hōʻike kēia papahana i kahi hui chromosome-scale o Miscanthus lutarioriparius genome ma o ka hoʻohui ʻana i nā ʻenehana Oxford Nanopore a me nā ʻenehana Hi-C.Hoʻopili ka hui 2.07Gb i ka 96.64% o ka genome, me ka contig N50 o 1.71 Mb.Ma kahi o 94.30% o ka huina pau i hoʻopaʻa ʻia i 19 pseudochromosomes.Ma o ka hoʻohālikelikeʻana me ka BAC sequence, LAI evaluation, BUSCO evaluation, re-assembly with NGS data, re-assembly of transcriptome data, the genome is evaluated as high quality and continuity.Hoʻopaʻa ʻia ke kumu Allotetraploid o ka M. lutarioriparius me ka hoʻohana ʻana i ka satellite centromeric repeats.ʻO Tandem duplicate genes o M. lutarioriparius ua hoʻonui ʻia ka hana ʻaʻole wale i nā huaʻōlelo e pili ana i ka pane ʻana i ke koʻikoʻi, akā ʻo ka biosynthesis o ka paia cell.Hiki paha i nā gene duplicate ke hana i ka C4 photosynthesis a hāʻawi i ka Miscanthus C4 photosynthesis ma ka wela haʻahaʻa.Ua hāʻawi ka noiʻi i mea nui no ke aʻo ʻana i nā mea kanu perennial.
6. Hāʻawi ʻia kahi hui genome Camptotheca acuminata pae chromosome i nā ʻike i ke kumu evolutionary o ka biosynthesis camptothecin
Mea Hana Hana: Ke Kulanui ʻo Sichuan
Nūpepa: Nature Communications
Ka hopena: 14.912
Ua hōʻike kēia papahana i kahi hui genome C. acuminata kūlana kiʻekiʻe, chromosome-level, me ka nui genome o 414.95Mb a me contingN50 1.47Mb.Ua ʻike mākou ua ʻike ʻo C. acuminata i kahi hoʻopiʻi kūʻokoʻa holoʻokoʻa-genome a he nui nā genes i loaʻa mai ia mea e pili ana i ka biosynthesis camptothecin.ʻO ka ʻokoʻa hana o ka gene LAMT a me ka hoʻololi maikaʻi ʻana o nā genes SLAS ʻelua, no laila, ua kōkua nui lāua ʻelua i ka biosynthesis camptothecin ma C. acuminata.Ua hōʻike ʻia nā hopena i ke koʻikoʻi o ka hui genome kiʻekiʻe i ka ʻike ʻana i nā loli genetic i ke kumu evolutionary o kahi metabolite lua.
7. Hōʻike ʻia ka genome i wehewehe ʻia e ka Allele i ka ʻokoʻa biallelic i ka wā o ka ulu ʻana o ka cassava
Mea Hana i hui pū ʻia: Ke Kula Nui ʻo Kina o ʻEpekema Mahiʻai Tropical
Nūpepa: Molecular Plant
Ka hopena: 13.162
Ua hōʻuluʻulu kēia papahana i kahi genome kuhikuhi no ka cassava me ka contigN50 1.1Mb me ka hoʻohana ʻana i ka paepae hoʻohālikelike ʻo Pacific Biosciences (PacBio).Ma hope o ka loiloi ʻana e BUSCO, LAI index, a me ka palapala moʻo kiʻekiʻe kiʻekiʻe, ua hoʻopaʻa ʻia ka genome i hui ʻia ma ke ʻano he refence-grade.Ua ʻike ʻia nā ʻāpana ʻē aʻe a ua kau ʻia nā contigs ma 18 pseudochromosomes me ka hoʻohana ʻana i nā loulou Hi-C.He mea waiwai kēia genome kiʻekiʻe a me ka allele-defined reference no ka cassava i ka ʻike ʻana i nā bi-allele ʻokoʻa ma nā chromosomes homologous, e ʻae ai e ʻimi i ka ʻokoʻa a me ka mana o ka bi-alleles a me kā lākou kumu hoʻokele evolutionary.Ua maʻalahi ia i ka hoʻomohala ʻana i nā hoʻolālā hoʻohua i ka cassava a me nā mea kanu heterozygous loa.
8. Nā ʻike Genomic i ka ulu wikiwiki ʻana o nā paulownias a me ka hoʻokumu ʻana o ka pulupulu kupua Paulownia
Mea Hana Hui: Henan Agricultural University
Nūpepa: Molecular Plant
Ka hopena: 13.162
Ua hōʻuluʻulu kēia papahana i kahi genome nuklea kiʻekiʻe o Paulownia fortunei, 511.6 Mb ka nui, me 93.2% o nā kaʻina i hoʻopaʻa ʻia i 20 pseudochromosomes.Loaʻa ka hana kiʻi kiʻi kiʻi kiʻekiʻe ma o ka hoʻohui ʻana i ka photosynthesis C3 a me ka crassulacean acid metabolism pathway, i kōkua paha i ka ulu wikiwiki loa o nā kumu lāʻau paulownia.ʻO ka hoʻopili genome hou o PaWB phytoplasma, i hui pū ʻia me nā loiloi hana, hōʻike ʻia e pili pono ana ka hopena PaWB-SAP54 me Paulownia PfSPLa, ka mea e hoʻohaʻahaʻa ai i ka PfSPLa e ke ala ubiquitin-mediated a alakaʻi i ka hoʻokumu ʻana i ka broom 'witch.Hāʻawi ka ʻikepili i nā ʻike koʻikoʻi i ka biology o paulownias a me ke ʻano hoʻoponopono no ka hoʻokumu ʻana o PaWB.
Genome holoholona - Nā ʻike hohonu o ka ulu ʻana o nā ʻano
1. ʻO ka genome o Nautilus pompilius e hoʻomālamalama i ka ulu ʻana o ka maka a me ka biomineralization
Mea Hana Hui: South China Sea Institute of Oceanology, CAS
Nūpepa: Natural Ecology & evolution
Ka hopena: 15.462
Hāʻawi kēia papahana i kahi genome piha no Nautilus pompilius.Loaʻa iā ia ka genome minimalist ma waena o nā cephalopods sequenced, ʻo ia ka 730.58Mb me contigN50 = 1.1Mb.ʻO ka hopena loiloi BUSCO he 91.31%.Hoʻohui pū ʻia me ka transcriptome, proteome, gene family a me phylogenetic analysis, ua hāʻawi kēia genome i kahi kuhikuhi kumu no nā hana hou cephalopod, e like me ka pinhole eye a me ka biomineralization.Ua hōʻike ʻia ka noiʻi e pili ana ka pōʻino i ka hoʻopiha piha ʻana o ka pūʻulu gene Hox i ka nalo ʻana o ka pūpū o Mollusks.ʻO ka mea nui, ʻo nā hana hou genomic he nui e pili ana i ka nalowale ʻana o ke ʻano, ka hoʻokaʻawale kūʻokoʻa a me ka hoʻonui ʻana o nā ʻohana gene kikoʻī a me kā lākou ʻoihana hoʻoponopono e pili ana i hoʻopololei i ka ulu ʻana o ka maka nautilus pinhole.He kumu waiwai ka nautilus genome no ke kūkulu hou ʻana i nā hiʻohiʻona evolutionary a me nā hana hou genomic e hoʻohālikelike ai i nā cephalopods e ola nei.
2. Hāʻawi ʻo Seadragon genome analysis i nā ʻike i kāna phenotype a me ka wahi hoʻoholo wahine.
Mea Hana Hui: South China Sea Institute of Oceanology, CAS
Nūpepa: Nā holomua ʻepekema
Ka hopena: 14.132
ʻO kēia papahana de novo-sequenced genomes kāne a wahine o ke kai maʻamau (Phyllopteryx taeniolatus) a me kona ʻano pili pili, ka alligator pipefish (Syngnathoides biaculeatus).ʻO ka nui genome no Phyllopteryx taeniolatus he ~659 Mb（♂） a me ~663 Mb（♀）, me ka contigN50 o 10.0Mb a me 12.1mb.ʻO ka nui genome no Phyllopteryx taeniolatus he 637 Mb（♂） a me ~648 Mb（♀）, me contigN50 o 18.0Mb a me 21.0Mb.Ma o ka phylogenetic analysis, ʻo ka seadragon maʻamau a me ka alligator pipefish he kaikuahine ʻauhau ʻo Syngnathinae, a ua hoʻokaʻawale ʻia ma kahi o 27.3 Ma mua.Hōʻike nā moʻolelo kope mai kahi mea hou evolutionary, nā ʻāpana like me ka lau, ua hoʻopili ʻia kahi pūʻulu o nā genes i komo maʻamau i ka hoʻomohala ʻana a me ka hoʻonui ʻana i nā kope no ka hoʻoponopono ʻana i ka ʻiʻo a me nā genes pale pale.Ua ʻike ʻia kahi locus e hoʻoholo ana i ka wahine e hoʻopili ana i kahi gene amhr2y kikoʻī kāne i kaʻana like ʻia e ka seadragon maʻamau a me ka alligator pipefish.Hāʻawi kēia papahana i nā hōʻike koʻikoʻi no ka noiʻi ʻana i ka evolution adaptive.
Ka manawa hoʻouna: Sep-19-2022