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T2T GENOME ASSEMBLY, GAP FREE GENOME

1stʻElua Raiki Genomes1

Title: Hui a me ka hōʻoia ʻana o ʻelua Gap-free Reference Genomes no Xian/indica Rice e hōʻike ana i nā ʻike i ka Plant Centromere Architecture

Doi:https://doi.org/10.1101/2020.12.24.424073

Ka manawa i kau ʻia: Ianuali 01, 2021.

Institute: Huazhong Agricultural University, Kina

Nā mea waiwai

O. sativa xian/indicaʻano laiki 'Zhenshan 97 (ZS97)' a me 'Minghui 63 (MH63)

Hoʻolālā kaʻina

Heluhelu NGS + Helu HiFi + Heluhelu CLR + BioNano + Hi-C

ʻIkepili:

ZS97: 8.34 Gb(~23x)HiFi heluhelu + 48.39 Gb (~131x) CLR heluhelu + 25 Gb(~69x) NGS + 2 BioNano Irys pūnaewele

MH63: 37.88 Gb (~103x) HiFi heluhelu + 48.97 Gb (~132x) CLR heluhelu + 28 Gb(~76x) NGS + 2 BioNano Irys pūnaewele

Kiʻi-1

Kiʻi 1 ʻElua mau ʻano lāʻau lapaʻau ʻole o ka laiki (MH63 a me ZS97)

2ndMaiʻa Genome2

Title: Telomere-to-telomere gapless chromosomes o ka maiʻa me ka hoʻohana ʻana i ke kaʻina nanopore

Doi:https://doi.org/10.1101/2021.04.16.440017

Ka manawa i kau ʻia: ʻApelila 17, 2021.

Ke kula: Université Paris-Saclay, Palani

Nā mea waiwai

haploid pāluaʻO Musa acuminatasppmalaccensis(DH-Pahang)

Hoʻolālā kaʻina a me ka ʻikepili:

ʻO ke ʻano HiSeq2500 PE250 + Minion/PromethION (93Gb,~200X)+ palapala ʻāina Optical (DLE-1+BspQ1)

Papa 1 Hoʻohālikelike o nā hui genome Musa acuminata (DH-Pahang).

Papa1-Hoʻohālikelike-o-GRCh38-a-T2T-CHM13-huihui-genome-kanaka
Kiʻi-Musa-genomes-architecture-hoʻohālikelike

Kiʻi 2 Musa genomes hoʻohālikelike hoʻohālikelike

3rdPhaeodactylum tricornutum genome3

Title: Telomere-to-telomere genome hui oP
haeodactylum tricornutum

Doi:https://doi.org/10.1101/2021.05.04.442596

Ka manawa i kau ʻia: Mei 04, 2021

Institute: Ke Kulanui o Western, Kanada

Nā mea waiwai

Phaeodactylum tricornutum(Culture Collection of Algae and Protozoa CCAP 1055/1)

Hoʻolālā kaʻina a me ka ʻikepili:

1 ʻO Oxford Nanopore minION pūnaewele kahe + he 2×75 paired-end mid-output NextSeq 550 holo

Kiʻi-Kaila-hana-no-telomere-a-telomere-genome-hui-1-1024x740

Kiʻi 3 Kaʻina hana no ka hui genome telomere-a-telomere

4thKanaka CHM13 genome4

Title: ʻO ke kaʻina piha o kahi genome kanaka

Doi:https://doi.org/10.1101/2021.05.26.445798

Ka manawa i kau ʻia: Mei 27, 2021

Institute: National Institutes of Health (NIH), USA

Mea lako: laina kelepona CHM13

Hoʻolālā kaʻina a me ka ʻikepili:

30× PacBio circular consensus sequencing (HiFi) , 120× Oxford Nanopore ultra-long read sequencing , 100× Illumina PCR-Free sequencing (ILMN) , 70× Illumina / Arima Genomics Hi-C (Hi-C), BioNano maps optical, a me Strand-seq

Papa 2 Hoʻohālikelike o GRCh38 a me T2T-CHM13 hui genome kanaka

Papa-Hoʻohālikelike-o-Musa-acuminata-DH-Pahang-genome-assemblies

Kuhikuhi

1.Sergey Nurk et al.ʻO ke kaʻina piha o kahi genome kanaka.bioRxiv 2021.05.26.445798;doi:https://doi.org/10.1101/2021.05.26.445798

2.Caroline Belser et al.Telomere-a-telomere gapless chromosomes o ka maiʻa me ka hoʻohana ʻana i ke kaʻina nanopore.bioRxiv 2021.04.16.440017;doi:https://doi.org/10.1101/2021.04.16.440017

3. Daniel J. Giguere et al.Telomere-to-telomere genome hui o Phaeodactylum tricornutum.bioRxiv 2021.05.04.442596;doi:https://doi.org/10.1101/2021.05.04.442596

4.Jia-Ming Song et al.ʻO ka hui ʻana a me ka hōʻoia ʻana o ʻelua Gap-free Reference Genomes no Xian/indica Rice e hōʻike ana i nā ʻike i ka Plant Centromere Architecture.bioRxiv 2020.12.24.424073;doi:https://doi.org/10.1101/2020.12.24.424073


Ka manawa hoʻouna: Jan-06-2022

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