ʻO ka genotyping kiʻekiʻe, ʻoi aku ka nui o ka heluna kanaka, he hana nui ia i ka noiʻi ʻana i ka hui genetic, e hāʻawi ana i ke kumu genetic no ka ʻike ʻana i nā ʻano hana, ka loiloi evolutionary, etc. ) ua hoʻokomo ʻia e hōʻemi i ke kumukūʻai kaʻina no kēlā me kēia laʻana, ʻoiai e mālama pono i ka hana kūpono ma ka ʻike ʻana i ka hōʻailona genetic.Loaʻa ʻia kēia ma ka unuhi ʻana i ka ʻāpana palena i loko o ka laulā nui i hāʻawi ʻia, ka mea i kapa ʻia ka hale waihona hōʻike hōʻemi (RRL).ʻO ka kikoʻī-locus amplified fragment sequencing (SLAF-Seq) kahi hoʻolālā i hoʻomohala ponoʻī no ka ʻike de novo SNP a me ka genotyping SNP o nā heluna nui.
Kaʻina hana ʻenehana
SLAF a me nā ʻano RRL e kū nei
Nā pōmaikaʻi o SLAF
ʻOi aku ka maikaʻi o ka loaʻa ʻana o ka hoailona genetic- Hoʻohui pū ʻia me ka ʻenehana sequencing kiʻekiʻe, hiki iā SLAF-Seq ke hoʻokō i nā haneli haneli o nā hōʻailona i ʻike ʻia i loko o ka genome holoʻokoʻa e hoʻokō ai i ke noi o nā papahana noiʻi like ʻole, me ka ʻole a i ʻole ka genome kuhikuhi.
Hoʻolālā hoʻokolohua maʻamau a maʻalahi- No nā pahuhopu noiʻi ʻokoʻa a i ʻole nā ʻano ʻano like ʻole, loaʻa nā hoʻolālā hoʻoheheʻe enzymatic ʻokoʻa me ka hoʻoheheʻe hoʻokahi-enzyme, pālua-enzyme a me ka multi-enzyme digestion.E loiloi mua ʻia ka hoʻolālā digestion ma ka silico e hōʻoia i kahi hoʻolālā enzyme maikaʻi loa.
ʻO ka hana kiʻekiʻe i ka digestion enzymatic- Hāʻawi ka hoʻoheheʻe enzymatic i hoʻolālā mua ʻia i nā SLAF i hoʻohele like ʻia ma ka chromosome.Hiki ke loaʻa ma luna o 95%.
Hōʻalo i ke kaʻina hana hou- Hoʻemi ʻia ka pākēneka o ke kaʻina hana hou i ka ʻikepili SLAF-Seq ma lalo o 5%, ʻoi aku hoʻi i nā ʻano me nā mea kiʻekiʻe o ka repetitive, e like me ka palaoa, ka maile, etc.
ʻO ke kaʻina hana bioinformatic hoʻomohala ponoʻī- Ua hoʻomohala ʻo BMK i kahi kaila hana bioinformatic i pili i ka ʻenehana SLAF-Seq e hōʻoia i ka hilinaʻi a me ka pololei o ka hopena hope.
Noi o SLAF
Palapala ʻāina pili genetic
Ke kūkulu ʻia ʻana o ka palapala ʻāina ʻano kiʻekiʻe a me ka ʻike ʻana i nā loci e hoʻomalu ana i nā ʻano pua ʻano pua ma Chrysanthemum(Chrysanthemum x morifolium Ramat.)
Nūpepa: Horticulture Research Paʻi ʻia: 2020.7
GWAS
ʻO ka ʻike ʻana i kahi moho moho e pili ana me ka ʻike isofavone i loko o nā kumulāʻau soya me ka hoʻohana ʻana i ka hui genome-ākea a me ka palapala ʻāina pili.
Nūpepa: ka Plant Journal i hoʻopuka ʻia: 2020.08
Nā Genetics Evolutionary
Hōʻike ka helu helu genomic a me ka hui de novo i ke kumu o ka laiki weedy ma ke ʻano he pāʻani evolutionary
Nūpepa: Molecular Plant Published: 2019.5
ʻIkepili hoʻokaʻawale nui (BSA)
ʻO GmST1, ka mea e hoʻopili ai i kahi sulfotransferase, hāʻawi i ke kū'ē i ka soybean mosaic virus strains G2 a me G3
Nūpepa: Mea kanu, Cell & Kaiapuni i Paʻi ʻia: 2021.04
Kuhikuhi
ʻO Sun X, Liu D, Zhang X, et al.SLAF-Seq: he ala kūpono o ka ʻike nui de novo SNP a me ka genotyping me ka hoʻohana ʻana i ka sequencing kiʻekiʻe-throughput [J].Plos one, 2013, 8(3):e58700
Mele X, Xu Y, Gao K, et al.Ke kūkulu ʻia ʻana o ka palapala ʻāina kiʻekiʻe kiʻekiʻe a me ka ʻike ʻana i nā loci e hoʻomalu ana i nā ʻano pua ʻano pua ma Chrysanthemum (Chrysanthemum × morifolium Ramat.).ʻO Hortic Res.2020;7:108.
Wu D, Li D, Zhao X, et al.Ka ʻike ʻana o kahi moho moho e pili ana me ka isoflavone ma loko o nā kumulāʻau soya me ka hoʻohana ʻana i ka hui genome-ākea a me ka palapala ʻāina pili.Mea kanu J. 2020;104(4): 950-963.
Sun J, Ma D, Tang L, et al.Hōʻike ka heluna kanaka genomic a me ka hui ʻo De Novo i ke kumu o ka weedy raiki ma ke ʻano he pāʻani evolutionary.Mea kanu Mol.2019;12(5):632-647.Mea kanu Mol.2018;11(11):1360-1376.
Zhao X, Jing Y, Luo Z, a me al.ʻO GmST1, ka mea e hoʻopili ai i ka sulfotransferase, hāʻawi i ke kū'ē i ka soybean mosaic virus strains G2 a me G3.Ka puni o na mea kanu.2021;10.1111/pce.14066
Ka manawa hoʻouna: Jan-04-2022