He mea koʻikoʻi ka ʻimi ʻana i nā hiʻohiʻona hōʻike gene ma kahi kumu o kahi mea ola no ka hoʻomaopopo ʻana i nā ʻano a me nā hana o kāna mau sela.Eia nō naʻe, loaʻa i nā ʻano loiloi transcriptome spatial i kēia manawa nā palena e like me ka haʻahaʻa haʻahaʻa a i ʻole ka lawa ʻole o ka hoʻonā.Hiki i ka BMKMANU S1000 Spatial Chip, i hoʻomohala ʻia e BMKMANU, hiki ke ʻike i ka ʻike hōʻike gene in-situ ma nā ʻāpana kiko piha i ka hoʻonā subcellular, e hiki ai i kahi wehewehe nuanced o ka hoʻolālā kiko.
E mākaukau e ʻike i kā mākou huahana hou loa!No ka hoʻolauleʻa ʻana i kona hoʻokuʻu ʻana, hāʻawi mākou i kahi kūikawāKūʻai ʻEhā Loaʻa Hoʻokahi manuahihoʻolaha no ka manawa palena wale.
Nā Mea Huahana
ʻO BMKMANU S1000 chip spatial chip a me ka pahu reagent like me:
1) ʻO ka Tissue Optimization Kit, i loaʻa nā reagents no ka hoʻomaʻamaʻa ʻana i ke kino (ka ʻimi ʻana i ka manawa kūpono no ka permeabilization kiko).
2) Gene Expression Kit, loaʻa nā reagents no ka hopu ʻana i ka mRNA i loko o nā ʻāpana kiko no ka hoʻomākaukau a me ka hoʻonohonoho ʻana o ka waihona.
3) Startup Kit: Loaʻa i kahi adapter thermostatic a me kahi transcriptome magnetic separator.
Kuʻuna ʻenehana
Hoʻokumu ʻia ka chip i nā micropores a me nā microbeads, a hiki ke hopu ʻia ka mRNA ma ke kahua ma o Oligo ma nā microbeads.He ʻehā ʻāpana ka Oligo: Read1, Spatial Barcode, UMI, a me Poly(dT)VN.Ma hope o ka hoʻopili ʻana o ka ʻiʻo i ka chip, hoʻokuʻu ʻia ka mRNA mai ka ʻiʻo e kahi enzyme permeabilization.No ka mea he huelo Poly-A ka hapa nui o ka mRNA 3', ua hopu ʻia lākou e Oligo me Poly(dT)VN.Ma hope o ka hoʻonui ʻana i ka RT-PCR a me ka cDNA, ka hoʻomākaukau ʻana o ka hale waihona puke a me ka hoʻonohonoho ʻana, loaʻa ka huli ʻana i ke kūlana spatial ma o Spatial Barcode.Hāʻawi kēia i ka nānā ʻana i ka hōʻike gene ma nā wahi kikoʻī a me ka heterogeneity o nā kiko.
Nā Pono Huahana
Nā noi
Hiki ke hoʻohana ʻia i ka hapa nui o ka noiʻi biological a me ka noiʻi lapaʻau, me ka ʻōpū, ka maʻi, ka immunology, a me ka biology developmental, e kōkua ana i ka hoʻokō ʻana i nā holomua hou i kēia mau kahua me ka nānā ʻana i ka hōʻike spatial ma ka hoʻonā subcellular.
●Tumor a me ka maʻi:
ʻO ka heterogeneity spatial a me ka microenvironment o nā maʻi maʻi a me nā maʻi
ʻO ka hoʻomaka a me ka uluʻana o nā maʻi maʻi
ʻO ka pane lapaʻau o nā ʻōpū a me nā maʻi
●Kōola hoʻomohala
Spatiotemporal atlas o nā kino
Nā hana hoʻoponopono Gene i ka wā o ka hoʻomohala ʻana
●Pane Stress
Pane pilikia biotic
Pane pilikia abiotic
●Immunology
Ka pane ʻana o ke kino i ka hoʻololi ʻana o ke kino
ʻO nā mīkini pale o nā maʻi a me nā maʻi
Pathogenesis o nā maʻi autoimmune
●Ka nānā 'ana i ka lā'au kū'ē
Mechanisms of drug resistance
Ka noiʻi a me ka hoʻomohala ʻana i nā lāʻau lapaʻau hou
Nā hihia a me ka ʻikepili Demo
Ua hōʻoia ʻia ʻo BMKMANU S1000 i nā haneli o nā hihia ma nā ʻano kikoʻī like ʻole.
