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proteomics
Hoʻopili ʻo Proteomics i nā noi o nā ʻenehana no ka helu ʻana i nā protein holoʻokoʻa i loaʻa i ka ʻike o kahi cell, ʻiʻo a i ʻole ke kino.Hoʻohana ʻia nā ʻenehana e pili ana i ka Proteomics i nā mana like ʻole no nā ʻano noiʻi like ʻole e like me ka ʻike ʻana i nā hōʻailona diagnostic like ʻole, nā moho no ka hana kano, ka hoʻomaopopo ʻana i nā ʻano pathogenicity, ka hoʻololi ʻana o nā hiʻohiʻona i ka pane ʻana i nā hōʻailona like ʻole a me ka wehewehe ʻana i nā ala protein hana i nā maʻi like ʻole.I kēia manawa, hoʻokaʻawale nui ʻia nā ʻenehana proteomic quantitative i TMT, Label Free a me DIA quantitative hoʻolālā.
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Metabolomics
ʻO ka metabolome ka huahana hope loa o ka genome a loaʻa iā ia ka hoʻopiha piha o nā molekala kaumaha haʻahaʻa haʻahaʻa (metabolites) i loko o kahi cell, tissue, a i ʻole ke kino.Manaʻo ka Metabolomics e ana i ka laulā ākea o nā molekole liʻiliʻi i loko o ka pōʻaiapili o ka hoʻoulu ʻana i ke kino a i ʻole nā kūlana maʻi.Hāʻule nā ʻano hana metabolomics i ʻelua pūʻulu ʻokoʻa: metabolomics ʻaʻole i hoʻopaʻa ʻia, kahi loiloi piha i manaʻo ʻia o nā analytes hiki ke ana i loko o kahi laʻana me nā mea ʻike ʻole kemika me ka hoʻohana ʻana i ka GC-MS/LC-MS, a me nā metabolomics i kuhikuhi ʻia, ke ana o nā pūʻulu i wehewehe ʻia o ke ʻano kemika a nā metabolites i hōʻike ʻia me ka biochemically.
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Ka nānā 'ana i ka Segregant Bulked
ʻO ka loiloi hoʻokaʻawale nui (BSA) kahi ʻenehana i hoʻohana ʻia e ʻike koke i nā hōʻailona genetic pili phenotype.ʻO ke kaʻina hana nui o BSA ke koho ʻana i ʻelua pūʻulu o nā kānaka me nā phenotypes kūʻē loa, e hui pū ana i ka DNA o nā kānaka a pau e hana i ʻelua pūʻulu DNA, e ʻike ana i nā kaʻina ʻokoʻa ma waena o nā loko ʻelua.Ua hoʻohana nui ʻia kēia ʻano hana i ka ʻike ʻana i nā hōʻailona genetic i pili ikaika ʻia e nā genes i manaʻo ʻia i loko o nā genome mea kanu/holoholona.
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DNA/RNA Sequencing - Nanopore Sequencer
ʻO ke kaʻina o ONT kahi mole hoʻokahi ʻenehana hōʻailona uila i ka manawa maoli e pili ana i nā nanopores, ʻo ke kumu hoʻohālikelike o kēlā me kēia paepae.E hoʻopaʻa ʻia ka DNA/RNA i kau ʻelua i ka protein nanoporous i hoʻokomo ʻia i loko o ka biofilm a wehe ʻia ma lalo o ke alakaʻi o ka protein motor, ma lalo o ka hana o ka ʻokoʻa uila mai nā ʻaoʻao ʻelua o ka biofilm, hele nā kaula DNA/RNA i ka protein channel nanopore i kekahi. uku.Ma muli o ka ʻokoʻa o nā waiwai kemika o nā kumu like ʻole ma ke kaula DNA/RNA, i ka wā e hele ai kahi base a i ʻole mole DNA ma ke kahawai nanopore, e hoʻololi ia i nā hōʻailona uila like ʻole.Ma kaʻikeʻana a me ka piliʻana i kēia mau hōʻailona, hiki ke heluʻia nāʻano kumu kūpono, a hiki ke hoʻopau i kaʻike manawa maoli o ke kaʻina.
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Ka hoʻokaʻina DNA/RNA -PacBio Sequencer
ʻO PacBio sequencing platform he papa kuhikuhi lōʻihi heluhelu ʻia, ʻike ʻia ʻo ia kekahi o nā ʻenehana Sequencing Third-Generation Sequencing (TGS).ʻO ka ʻenehana koʻikoʻi, hoʻokahi-molele manawa maoli (SMRT), hoʻoikaika i ka hana o nā heluhelu me nā ʻumi kilo-base ka lōʻihi.Ma ke kumu o "Sequencing-by-Synthesis", ua hoʻokō ʻia ka hoʻonā nucleotide hoʻokahi e Zero-mode waveguide (ZMW), kahi i hoʻomālamalama ʻia ai ka leo liʻiliʻi ma lalo (kahi o ka mole synthesis).Eia hou, pale ka SMRT sequencing nui i ka sequence-specific bias in NGS system, no ka mea, ʻaʻole koi ʻia ka hapa nui o nā ʻanuʻu PCR amplification ma ke kaʻina hana hale waihona puke.
Papahana: Sequel II, Revio
