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Mea kanu/holoholona De novo Genome Sequencing
De novoʻO ke kaʻina hana e pili ana i ke kūkulu ʻana i ka genome holoʻokoʻa o kekahi ʻano me ka hoʻohana ʻana i nā ʻenehana hoʻohālikelike, e laʻa me PacBio, Nanopore, NGS, etc., me ka loaʻa ʻole o kahi genome kuhikuhi.ʻO ka hoʻomaikaʻi maikaʻi ʻana i ka lōʻihi heluhelu o nā ʻenehana sequencing ʻekolu ua lawe mai i nā manawa hou i ka hui ʻana i nā genomes paʻakikī, e like me nā mea me ka heterozygosity kiʻekiʻe, ka ratio kiʻekiʻe o nā ʻāpana repetitive, polyploids, etc. ka hoʻoholo ʻana i nā mea hana hou, nā ʻāpana me nā mea ʻokoʻa GC a me nā wahi paʻakikī ʻē aʻe.
Papahana: PacBio Sequel II / Nanopore PromethION P48/ Illumina NovaSeq6000
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ʻO ka Genome Assembly ma muli o Hi-C
He ʻano hana ʻo Hi-C no ka hopu ʻana i ka hoʻonohonoho chromosome ma o ka hoʻohui ʻana i nā pilina pili pili kokoke a me ke kaʻina hana kiʻekiʻe.ʻO ka ikaika o kēia mau pilina i manaʻo ʻia ua pili ʻole ʻia me ka mamao kino ma nā chromosomes.No laila, hiki i ka ʻikepili Hi-C ke alakaʻi i ka hui ʻana, ka hoʻonohonoho ʻana a me ka hoʻonohonoho ʻana o nā kaʻina i hui ʻia i loko o kahi genome kikoʻī a hoʻopaʻa i kēlā mau mea ma kekahi helu o nā chromosomes.Hāʻawi kēia ʻenehana i kahi hui genome pae chromosome me ka loaʻa ʻole o ka palapala ʻāina moʻo.Pono kēlā me kēia genome i kahi Hi-C.
Papahana: Illumina NovaSeq6000 / DNBSEQ
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Nā Genetics Evolutionary
ʻO ka Evolutionary genetics kahi lawelawe hoʻonohonoho i hoʻopaʻa ʻia no ka hāʻawi ʻana i ka wehewehe piha ʻana i ka ʻike evolutionary o nā mea i hāʻawi ʻia e pili ana i nā ʻano like ʻole, me nā SNP, InDels, SV a me CNV.Hāʻawi ia i nā loiloi kumu āpau e pono ai no ka wehewehe ʻana i nā loli evolutionary a me nā hiʻohiʻona genetic o nā heluna kanaka, e like me ke ʻano o ka heluna kanaka, ka ʻokoʻa genetic, pili phylogeny, a me nā mea ʻē aʻe.
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Hoʻohālikelike Genomics
ʻO ke ʻano o ka hoʻohālikelike ʻana i ka genome ka hoʻohālikelike ʻana i nā kaʻina genome piha a me nā hale o nā ʻano ʻano like ʻole.Ke manaʻo nei kēia aʻo ʻana i ka hōʻike ʻana i ka ulu ʻana o nā ʻano mea, ka hana gene, ka ʻōnaehana hoʻoponopono gene ma ka pae genome ma ka ʻike ʻana i nā ʻano hana a me nā mea i mālama ʻia a hoʻokaʻawale ʻia ma nā ʻano like ʻole.ʻO ka haʻawina genomics maʻamau e pili ana i nā loiloi i ka ʻohana gene, ka hoʻomohala ʻana i ka evolutionary, ka duplication genome holoʻokoʻa, ke kaomi koho, etc.
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Ka nānā 'ana i ka Segregant Bulked
ʻO ka Bulked segregant analysis (BSA) kahi ʻenehana i hoʻohana ʻia e ʻike koke i nā hōʻailona genetic pili phenotype.Loaʻa i ke kaʻina hana nui o BSA ke koho ʻana i ʻelua pūʻulu kanaka me nā phenotypes kūʻē loa, e hoʻohui i ka DNA o kēlā me kēia kanaka e hana i ʻelua pūʻulu DNA, e ʻike ana i nā kaʻina ʻokoʻa ma waena o nā loko ʻelua.Ua hoʻohana nui ʻia kēia ʻano hana i ka ʻike ʻana i nā hōʻailona genetic i pili ikaika ʻia e nā ʻano genes i manaʻo ʻia i loko o nā genomes mea kanu/holoholona.
