Nā huahana

Fungal Genome

Hāʻawi ʻo Biomarker Technologies i ka loiloi genome, genome maikaʻi a me ka genome pene-complete o ka fungal ma muli o ka pahuhopu noiʻi kikoʻī.Hiki ke hoʻokō ʻia ke kaʻina o Genome, ka hui ʻana a me ka hōʻike hana ma o ka hoʻohui ʻana i ke kaʻina o ka hanauna aʻe + ʻO ke kaʻina o ka hanauna ʻekolu e hoʻokō i ka hui genome kiʻekiʻe.Hiki ke hoʻohana ʻia ka ʻenehana Hi-C e hoʻomaʻamaʻa i ka hui genome ma ka pae chromosome.

Papahana:PacBio Sequel II

Nanopore PromethION P48

ʻO Illumina NovaSeq Platform

Kāhea iā mā˚ou

Nā kikoʻī lawelawe

Nā hopena hōʻike

Pono lawelawe

● Loaʻa nā hoʻolālā hoʻonohonoho hoʻonohonoho lehulehu no nā pahuhopu noiʻi like ʻole

● Loaʻa i ka ʻike i ka hui genome fungi me ka ʻoi aku o 10,000 pene-complete genome i hui ʻia.

● 'Oihana ma hope o ke kū'ai 'ana i ke kāko'o 'oihana hui e ho'okō ana i nā pono noi'i kiko'ī.

Nā kikoʻī lawelawe

lawelawe	Hoʻolālā Sequencing	Paʻa ka maikaʻi	Kuhi ʻia ka manawa huli
Palapala ʻāina maikaʻi fungal	Illumina 50X+Nanopore 100X	Contig N50≥2 Mb	35 mau lā hana
Palapala ʻāina maikaʻi fungal	PacBio HiFi 30X	Contig N50≥2 Mb	35 mau lā hana
ʻO ka palapala ʻāina paʻa pene fungal	Illumina 50X+Nanopore 100X(Pacbio HiFi 30X)+Hi-C 100X	Lakiō hoʻopaʻa Chromosome >90%	45 mau lā hana

Nā loiloi bioinformatics

● Ka hoʻomalu maikaʻi ʻikepili maka

● Hui Genome

● Ke kālailai ʻāpana genome

● Hōʻike hana Gene

● Hoʻohālikelike genomic analysis

Nā Koina Laʻana a me ka Hoʻouna ʻana

Nā Koina Laʻana:

No ka meaNā unuhi DNA:

ʻAno Laʻana	Ka nui	Hoʻopaʻa ʻana	Maemae
Nā unuhi DNA	＞ 1.2 μg	＞ 20 ng/μl	OD260/280= 1.6-2.5

No nā laʻana kiko:

ʻAno laʻana

Manaʻo laʻana lapaʻau

Laʻana mālama a hoʻouna

ʻAhaʻo Unicellular

E nānā i ka hū ma lalo o ka microscope a hōʻiliʻili iā lākou i ko lākou manawa exponential

E hoʻololi i ka moʻomeheu (loaʻa ma kahi o 3-4.5e9 mau keena) i loko o kahi eppendorf 1.5 a i ʻole 2 ml.(E hoomau i ka hau)

Centrifuge i ka paipu no 1 min ma 14000 g e hōʻiliʻili i ka hua bacteria a wehe pono i ka supernatant

E hoʻopaʻa i ka paipu a hoʻokuʻu i ka hua bacteria i loko o ka hau hau no ka liʻiliʻi o 1-3 mau hola.E mālama i ka paipu i loko o -80 ℃ friji.

E hoʻokuʻu i nā laʻana i loko o ka hau hau no 3-4 mau hola a e mālama i loko o ka wai nitrogen a i ʻole -80 degere a hiki i ka mālama lōʻihi.Pono ka hoʻouna laʻana me ka hau maloʻo.

ʻAiʻo Macro

Paipai ʻia ka ʻili i ka wā e ulu ikaika ana.

Holoi i ka ʻiʻo me ka wai ʻole endotoxin, a laila 70% ethonal.

E mālama i ka hāpana i loko o nā pahu cryo.