Nā huahana

DNA/RNA Sequencing - Nanopore Sequencer

ʻO ke kaʻina o ONT kahi mole hoʻokahi ʻenehana hōʻailona uila i ka manawa maoli e pili ana i nā nanopores, ʻo ke kumu hoʻohālikelike o kēlā me kēia paepae.E hoʻopaʻa ʻia ka DNA/RNA i kau ʻelua i ka protein nanoporous i hoʻokomo ʻia i loko o ka biofilm a wehe ʻia ma lalo o ke alakaʻi o ka protein motor, ma lalo o ka hana o ka ʻokoʻa uila mai nā ʻaoʻao ʻelua o ka biofilm, hele nā kaula DNA/RNA i ka protein channel nanopore i kekahi. uku.Ma muli o ka ʻokoʻa o nā waiwai kemika o nā kumu like ʻole ma ke kaula DNA/RNA, i ka wā e hele ai kahi base a i ʻole mole DNA ma ke kahawai nanopore, e hoʻololi ia i nā hōʻailona uila like ʻole.Ma kaʻikeʻana a me ka piliʻana i kēia mau hōʻailona, hiki ke heluʻia nāʻano kumu kūpono, a hiki ke hoʻopau i kaʻike manawa maoli o ke kaʻina.

Kāhea iā mā˚ou

Nā kikoʻī lawelawe

Ka hopena Demo

Nā hiʻohiʻona kikoʻī lawelawe

Papahana	Nui o ka waihona	HuaʻIkeʻIke Manaʻo (Per Cell)	Hoʻokahi-kumu pololei	Nā noi
Nanopore	8Kb, 10kb, 20kb, Ultralong, cDNA-PCR	70-90Gb/Celewele	85-92%	Kāhea SV, De novo, Ke kaʻina holoʻokoʻa holoʻokoʻa, Iso-Seq, Hōʻike Gene, ʻike i ka methylation DNA

Pono lawelawe

● ʻOi aku ma mua o 5 mau makahiki o ka ʻike ma PacBio sequencing platform me nā tausani o nā papahana i pani ʻia me nā ʻano ʻano like ʻole.
● ʻO BMKGENE kekahi hoa pili o Oxford Nanopore, me ka palapala hōʻoia RNA/DNA papalua.
● Loaʻa nā hiʻohiʻona nui o nā sequencers me nā lako piha a me ka lawa o ka sequencing throughput.
● Ma muli o ka Nanopore platform, ʻoi aku ma mua o 10 mau noiʻi holoholona a me nā mea kanu Denovo i paʻi ʻia i loko o nā nūpepa kaulana honua.

Nā Koina Laʻana

ʻAno Laʻana

Ka nui

ʻO ka manaʻo (Qubit ®)

Volume

Maemae

ʻO nā mea ʻē aʻe

DNA Genomic

E hilinaʻi i ka ʻikepili koi

≥20ng/μl

≥15μl

OD260/280=1.7-2.2;

OD260/230≥1.5；

Maʻemaʻe ka piko ma 260 nm， ʻaʻohe haumia

Pono e ana ʻia ka manaʻo e Qubit a me Qubit/Nanopore ≤ 2

Huina RNA

≥1.2μg

≥100μg/μl

≥15μl

OD260/280=1.7-2.5;

OD260/230=0.5-2.5; ʻaʻohe haumia

Waiwai RIN ≥7.5

Hana Hana Hana

Hoʻomākaukau laʻana

Kūkulu hale waihona puke

Ke kaʻina ʻana

ʻIkepili ʻikepili

Hāʻawi papahana

Mua: DNA/RNA Sequencing - Illumina Sequencer

Aʻe: ʻO ke kaʻina o nā ʻāpana ʻāpana kikoʻī i hoʻonui ʻia (SLAF-Seq)

ʻIke ʻIke maikaʻi o ka hōʻailona DNA

Papa 1. Heluhelu ma ka ikepili maemae.

BMKID	rawSeqNum	rawSumBase	cleanSeqNum	cleanSumBase	cleanN50Len	cleanN90Len	cleanMeanLen	cleanMaxLen	cleanMeanQual
DNA_BMK01	1,218,239	26.37	1,121,736	25.90	28,014	15,764	23,090	143,181	9