Nā huahana

Illumina a me BGI

ʻO ka ʻenehana Sequencing Illumina, i hoʻokumu ʻia ma Sequencing by Synthesis (SBS), he hana hou NGS i kākoʻo ʻia i ka honua, kuleana no ka hoʻomohala ʻana ma luna o 90% o ka ʻikepili sequencing o ka honua.ʻO ke kumumanaʻo o SBS e pili ana i ke kiʻi ʻana i ka fluorescently labeled reversible terminator i ka hoʻohui ʻia ʻana o kēlā me kēia dNTP, a ma hope i hoʻokaʻawale ʻia e ʻae i ka hoʻohui ʻana i ka waihona aʻe.Me nā dNTP i hoʻopaʻa ʻia e hoʻohuli hou ʻia i kēlā me kēia pōʻaiapili kaʻina, hoʻemi ka hoʻokūkū kūlohelohe i ka hoʻohui ʻana.Kākoʻo kēia ʻenehana loea i nā hale waihona puke heluhelu hoʻokahi a me nā hale waihona puke hoʻokahi, e mālama ana i nā ʻano hana genomic.Hiki i ka Illumina sequencing ke kiʻekiʻe a me ka pololei ke hoʻonoho iā ia ma ke ʻano he pōhaku kihi i ka noiʻi genomics, e hāʻawi i ka mana i nā ʻepekema e wehe i nā mea paʻakikī o nā genomes me nā kikoʻī like ʻole a me ka maikaʻi.

ʻO DNBSEQ, i hoʻomohala ʻia e BGI, ʻo ia kekahi ʻenehana NGS hou i hoʻokō i ka hoʻohaʻahaʻa ʻana i nā kumukūʻai sequencing a hoʻonui i ka throughput.ʻO ka hoʻomākaukau ʻana i nā hale waihona puke DNBSEQ e pili ana i ka ʻāpana DNA, ka hoʻomākaukau ʻana i ka ssDNA a me ka hoʻonui ʻia ʻana o ka pōʻai no ka loaʻa ʻana o nā DNA nanoballs (DNB).Hoʻouka ʻia kēia mau mea ma luna o kahi ʻili paʻa a hoʻopaʻa ʻia e ka hui ʻana o Probe-Anchor Synthesis (cPAS).

Hoʻomaʻamaʻa kā mākou lawelawe hoʻonohonoho hale waihona puke i nā mea kūʻai aku i ka hoʻomākaukau ʻana i kā lākou sequencing hale waihona puke mai nā kumu like ʻole (mRNA, whole genome, amplicon, a me nā mea ʻē aʻe).A laila, hiki ke hoʻouna ʻia kēia mau hale waihona puke i kā mākou mau kikowaena sequencing no ka hoʻokele maikaʻi ʻana a me ka hoʻonohonoho ʻana ma Illumina a i ʻole BGI platform.

Kāhea iā mā˚ou

Nā kikoʻī lawelawe

Ka hopena Demo

Nā hiʻohiʻona

●Nā papahana:Illumina NovaSeq 6000, NovaSeq , HiSeq X Ten a me BGI-DNB-T7

●Ke kaʻina hana:PE50, PE100, PE150, PE250

●Ka hoʻomalu maikaʻi ʻana o nā hale waihona puke ma mua o ka hoʻonohonoho ʻana

●Ke kaʻina nei i ka hāʻawi ʻana i ka ʻikepili a me QC:ka hāʻawi ʻana i ka hōʻike QC a me ka ʻikepili maka ma ka format fastq ma hope o ka demultiplexing a me ka kānana ʻana i nā heluhelu Q30.

Pono lawelawe

●Ka nui o nā lawelawe Sequencing:hiki i ka mea kūʻai ke koho i ke kaʻina ma ke ala, kahe kelepona a i ʻole ka nui o ka ʻikepili.

●Versatility o nā paepae:Hiki ke hoʻoili ʻia nā hale waihona puke DNB i nā paepae Illumina

●ʻO ka ʻike nui ma ke kahua hoʻonohonoho Illumina:me nā tausani o nā papahana pani me nā ʻano ʻano like ʻole.

●Ka hoʻouna ʻana i ka hōʻike QC:me nā anana maikaʻi, ka pololei o ka ʻikepili a me ka hana holoʻokoʻa o ka papahana sequencing.

●Kaʻina kaʻina hana oʻo:me ka manawa hoohuli pokole.

●Hoʻomalu maikaʻi loa: hoʻokō mākou i nā koi QC koʻikoʻi e hōʻoia i ka hāʻawi ʻana i nā hopena kiʻekiʻe mau.

Nā Koina Laʻana*

Hoʻokaʻina ʻĀpana Alanui

Ka nui o ka ʻikepili (X)	Hoʻopaʻa ʻia (qPCR/nM)	Volume
X ≤ 50 Gb	≥ 2 nM	≥ 20 μl
50 Gb ≤ X < 100 Gb	≥ 3 nM	≥ 20 μl
X ≥ 100 Gb	≥ 4 nM	≥ 20 μl

Alanui Hoʻokahi (Illumina)

Papahana	Hoʻopaʻa ʻia (qPCR/nM)	Volume
HiSeq X Umi	≥ 2 nM	≥ 20 μl
NovaSeq 6000 SP	≥ 1 nM	≥ 25 μl
NovaSeq 6000 S4	≥ 1.5 nM	≥ 25 μl
ʻO NovaSeq X	≥ 1.5 nM	≥ 25 μl
BGI-DNBSEQ-T7	≥ 1.5 nM	≥ 25 μl

Ma waho aʻe o ka noʻonoʻo ʻana a me ka nui o ka nui, pono pū kekahi kumu kiʻekiʻe kūpono.

E ʻoluʻolu e kelepona mai iā mākou inā ʻaʻole kūpono kāu mau laʻana i nā koi mea hoʻomaka.

Kāhea iā mā˚ou

Hana Hana Hana

Ka hoʻomalu ʻana i ka maikaʻi o ka waihona

Ke kaʻina ʻana

Ka hoʻomalu maikaʻi ʻikepili

Hāʻawi papahana

Mua: Ka hoʻokaʻina DNA/RNA -PacBio Sequencer

Aʻe: DNA/RNA Sequencing - Nanopore Sequencer

Hōʻike QC waihona

Hāʻawi ʻia kahi hōʻike e pili ana i ka maikaʻi o ka waihona ma mua o ka hoʻonohonoho ʻana, ka helu ʻana i ka nui o ka waihona, a me ka ʻāpana.

Hōʻike hōʻike QC

Papa 1. Heluhelu no ka hoʻokaʻina ʻikepili.

Laʻana ID	BMKID	Heluhelu maka	ʻIkepili Raw (bp)	Heluhelu maʻemaʻe (%)	Q20(%)	Q30(%)	GC(%)
C_01	BMK_01	22,870,120	6,861,036,000	96.48	99.14	94.85	36.67
C_02	BMK_02	14,717,867	4,415,360,100	96.00	98.95	93.89	37.08